Institutional Repository of Chinese Acad Sci, Inst Solid State Phys， Hefei 230031, Anhui, Peoples R China
| Magneto-plamonic nanoparticles enhanced surface plasmon resonance TB sensor based on recombinant gold binding antibody | |
| Zou, Fei1; Wang, Xingxin1,2; Qi, Fangjie1; Kohn, Kwangnak3; Lee, Jaebeom4,5; Zhou, Hongjian6; Chen, Hongxia1 | |
| 2017-10-01 | |
| 发表期刊 | SENSORS AND ACTUATORS B-CHEMICAL
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| 摘要 | The magneto-plamonic nanoparticles (MPNs) have been employed to enhance the signal of surface plasmon resonance (SPR) spectroscopy to gain new insight into MPN and gold film SPR interaction. Three different morphologies MPNs (sphere, short spiky and long spiky) of Fe3O4@Au nanoparticles with good dispersion were synthesized and characterized carefully. A sandwich SPR immunosensor was constructed by immobilizing gold binding anti-CFP-10 (Ab1) onto a gold chip surface via Au-S bond firstly. Gold binding anti-CFP-10 (Ab2) captured on MPN surface was utilized to amplify the SPR signals specifically. Compared with spiky MPNs, the structure of spherical MPN, which concentrates the electric charge density and immobilize more Abs on its surface coverage, could enhance the electronic coupling effect significantly. Attribute to the super paramagnetism of MPNs, a facile solution route was fabricated to capture and separate analyte from the real sample by outside extend magnetic field. Implementation of MPNs results in 30-fold enlargement of the SPR signal at the limit of detection. To this end, an immunoassay is carried out that couples the specificity of antibody-antigen interactions with the high sensitivity based on spherical MPNs signal enhancement SPR. (C) 2017 Elsevier B.V. All rights reserved. |
| 文章类型 | Article |
| 关键词 | Cfp-10 Spr Signal Amplification Magneto-photonic Nanoparticle |
| WOS标题词 | Science & Technology ; Physical Sciences ; Technology |
| DOI | 10.1016/j.snb.2017.04.162 |
| 关键词[WOS] | PROPYLIC ACID) FILM ; MYCOBACTERIUM-TUBERCULOSIS ; IN-SITU ; PROTEIN ; CFP-10 ; SPECTROSCOPY ; MICROSCOPY ; DIAGNOSIS ; RESPONSES ; ASSAY |
| 收录类别 | SCI |
| 语种 | 英语 |
| 项目资助者 | National Natural Science Foundation of China(61275085) ; National Natural Science Foundation of China(61275085) ; National Natural Science Foundation of China(61275085) ; National Natural Science Foundation of China(61275085) |
| WOS研究方向 | Chemistry ; Electrochemistry ; Instruments & Instrumentation |
| WOS类目 | Chemistry, Analytical ; Electrochemistry ; Instruments & Instrumentation |
| WOS记录号 | WOS:000404424100043 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.hfcas.ac.cn:8080/handle/334002/32003 |
| 专题 | 中科院固体物理研究所 |
| 作者单位 | 1.Shanghai Univ, Sch Life Sci, Lab Biosensing Technol, Shanghai 200444, Peoples R China 2.Shanghai Univ, Sch Life Sci, Shanghai Key Lab Bioenergy Crops, Shanghai 200444, Peoples R China 3.Pusan Natl Univ, Inst Gen Educ, Busan 609735, South Korea 4.Pusan Natl Univ, Dept Nanofus Engn, Busan 609735, South Korea 5.Pusan Natl Univ, Dept Cognomechatron Engn, Busan 609735, South Korea 6.Chinese Acad Sci, Inst Solid State Phys, Key Lab Mat Phys, Hefei 230031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zou, Fei,Wang, Xingxin,Qi, Fangjie,et al. Magneto-plamonic nanoparticles enhanced surface plasmon resonance TB sensor based on recombinant gold binding antibody[J]. SENSORS AND ACTUATORS B-CHEMICAL,2017,250(无):356-363. |
| APA | Zou, Fei.,Wang, Xingxin.,Qi, Fangjie.,Kohn, Kwangnak.,Lee, Jaebeom.,...&Chen, Hongxia.(2017).Magneto-plamonic nanoparticles enhanced surface plasmon resonance TB sensor based on recombinant gold binding antibody.SENSORS AND ACTUATORS B-CHEMICAL,250(无),356-363. |
| MLA | Zou, Fei,et al."Magneto-plamonic nanoparticles enhanced surface plasmon resonance TB sensor based on recombinant gold binding antibody".SENSORS AND ACTUATORS B-CHEMICAL 250.无(2017):356-363. |
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