A novel method for assessing the toxicity of silver nanoparticles in Caenorhabditis elegans

doi:10.1016/j.chemosphere.2016.11.011

	A novel method for assessing the toxicity of silver nanoparticles in Caenorhabditis elegans
	Luo, Xun 1,2,4; Xu, Shengmin 1,3; Yang, Yaning 1,3; Zhang, Yajun 1,3; Wang, Shunchang 4; Chen, Shaopeng1,3 ; Xu, An1,3 ; Wu, Lijun1,2,3
	2017-02-01
发表期刊	CHEMOSPHERE
摘要	At present, nanotechnology has been producing nanoscale materials with unprecedented speed. Nano materials could be inevitably released into the environment owing to their widespread use, and their potential toxicity has caused a great concern. With regard to assessment of nanomaterial toxicity, many studies probably don't truly reflect their toxicity, because the nanoparticles were not stable and uniformly dispersed in the medium. In the present study, the semi-fluid nematode growth gelrite medium (NGG) was used to achieve better distribution of silver nanoparticles (AgNPs). We aimed to evaluate the toxicity of AgNPs in three different culture methods, such as the NGG, nematode growth medium (NGM) and K-medium (KM). Our transmission electron microscopy, hydrodynamic diameter, and inductively coupled plasma-atomic emission spectrometry results demonstrated that AgNPs homogeneously and stably dispersed in NGG compared to that in liquid KM. Furthermore, the conventional toxicity end points, such as body length, fecundity, lifespan, population growth, germline cell apoptosis, reactive oxygen species, and mitochondrial membrane potential were used to assess the toxicity of AgNPs to Caenorhabditis elegans (C. elegans) in NGG, NGM and KM. Our results showed that the toxicity of AgNPs obtained in the NGG test medium was much higher than that in the standard NGM and KM. In addition to the improved dispersion of nanoparticles, NGG also offered advantages for long-term studies and likely provided a convenient nematode toxicity testing method. These results revealed that the NGG test medium was a suitable and sensitive culture method for the evaluation of AgNPs toxicity using C elegans. (C) 2016 Elsevier Ltd. All rights reserved.
文章类型	Article
关键词	Ngg Agnps Toxicity Germ Cell Apoptosis C. Elegans Nanotoxicity
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1016/j.chemosphere.2016.11.011
关键词[WOS]	OXIDATIVE STRESS ; GRAPHENE OXIDE ; DNA-DAMAGE ; NEMATODE ; GROWTH ; TOXICOLOGY ; APOPTOSIS ; EXPOSURE ; BEHAVIOR ; CELLS
收录类别	SCI
语种	英语
项目资助者	National Basic Research 973 Program(2014CB932002) ; National Basic Research 973 Program(2014CB932002) ; National Basic Research 973 Program(2014CB932002) ; National Basic Research 973 Program(2014CB932002) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDB14030502) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDB14030502) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDB14030502) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDB14030502) ; National Natural Science Foundation of China(81301182) ; National Natural Science Foundation of China(81301182) ; National Natural Science Foundation of China(81301182) ; National Natural Science Foundation of China(81301182)
WOS研究方向	Environmental Sciences & Ecology
WOS类目	Environmental Sciences
WOS记录号	WOS:000391897500078
引用统计	被引频次：25[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.hfcas.ac.cn:8080/handle/334002/32789
专题	技术生物与农业工程研究所
作者单位	1.Chinese Acad Sci, Key Lab Ion Beam Bioengn, Hefei Inst Phys Sci, Hefei 230031, Anhui, Peoples R China 2.Univ Sci & Technol China, Sch Life Sci, Hefei 230026, Anhui, Peoples R China 3.Key Lab Environm Toxicol & Pollut Control Technol, Hefei 230031, Anhui, Peoples R China 4.Huainan Normal Univ, Sch Bioengn, Huainan 232038, Anhui, Peoples R China
推荐引用方式 GB/T 7714	Luo, Xun,Xu, Shengmin,Yang, Yaning,et al. A novel method for assessing the toxicity of silver nanoparticles in Caenorhabditis elegans[J]. CHEMOSPHERE,2017,168(无):648-657.
APA	Luo, Xun.,Xu, Shengmin.,Yang, Yaning.,Zhang, Yajun.,Wang, Shunchang.,...&Wu, Lijun.(2017).A novel method for assessing the toxicity of silver nanoparticles in Caenorhabditis elegans.CHEMOSPHERE,168(无),648-657.
MLA	Luo, Xun,et al."A novel method for assessing the toxicity of silver nanoparticles in Caenorhabditis elegans".CHEMOSPHERE 168.无(2017):648-657.

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