The change of the state of cell membrane can enhance the synthesis of menaquinone in Escherichia coli

doi:10.1007/s11274-017-2222-9

	The change of the state of cell membrane can enhance the synthesis of menaquinone in Escherichia coli
	Liu, Yan 1,2,3; Ding, Xiu-min 1; Xue, Zheng-lian 1; Hu, Liu-xiu 1; Zhang, Ning-juan 1; Wang, Zhou 1; Yang, Jian-wei 1; Cheng, Qian 1; Chen, Ming-hong 1; Zhang, Zhuang-zhuang 3; Zheng, Zhi-ming2
	2017-03-01
发表期刊	WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
摘要	Menaquinone (MK) was an attractive membrane-bound intracellular chemical. To enhance its production, we tried to find the relationship between its synthesis and the state of cell membrane in producing strain. Due to non-ionic surfactant-polyoxyethylene oleyl ether (POE) and plant oil-cedar wood oil (CWO) can typically increase extracellular secretion and intracellular synthesis of MK respectively, the effect of these two substances on cell morphology, physical properties of cell membrane was investigated. Finally, two engineering strains were constructed to verify whether the state of cell membrane can enhance MK synthesis. The result showed that the edge of cells was broken when POE added in the medium. Other physical properties such as total fatty acid content decreased by 40.7% and the ratio of saturated fatty acids to unsaturated fatty acids decreased from 1.58 +/- 0.05 to 1.31 +/- 0.04. Mean-while, cell membrane leakage was enhanced from 7.14 to 64.31%. Different from POE group, cell membrane was intact in CWO group. Moreover, the ratio of saturated fatty acids to unsaturated fatty acids increased from 1.58 +/- 0.05 to 1.78 +/- 0.04 and the average lipid length decreased from 16.05 +/- 0.08 to 15.99 +/- 0.10. Two constructed strains, especially Escherichia coli DH5 alpha FatB, exhibited strong MK secretion ability and the extracellular MK reached 10.71 +/- 0.19 mg/L. An understanding of these functionary mechanisms could not only provide a new idea for the synthesis of MK, but also provide a reference to increase the yield of intracellular membrane-bound metabolites.
文章类型	Article
关键词	Menaquinone Cell Membranes Membrane Fluidity Leakage
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1007/s11274-017-2222-9
关键词[WOS]	FREE FATTY-ACIDS ; VITAMIN-K-2 ; SUPPLEMENTATION ; THIOESTERASE ; PATHWAY ; MODEL
收录类别	SCI
语种	英语
项目资助者	National Nature Science Foundation of China(31501465 ; National Nature Science Foundation of China(31501465 ; National Nature Science Foundation of China(31501465 ; National Nature Science Foundation of China(31501465 ; National High-tech RD Program(2014AA021704) ; National High-tech RD Program(2014AA021704) ; National High-tech RD Program(2014AA021704) ; National High-tech RD Program(2014AA021704) ; Anhui Provincial Nature Science Foundation(1608085QC54) ; Anhui Provincial Nature Science Foundation(1608085QC54) ; Anhui Provincial Nature Science Foundation(1608085QC54) ; Anhui Provincial Nature Science Foundation(1608085QC54) ; Anhui Higher Education Exchange Program for Young Talents(gxfxZD2016109) ; Anhui Higher Education Exchange Program for Young Talents(gxfxZD2016109) ; Anhui Higher Education Exchange Program for Young Talents(gxfxZD2016109) ; Anhui Higher Education Exchange Program for Young Talents(gxfxZD2016109) ; Anhui Provincial Undergraduate Innovation and Entrepreneurship Program(201510363130) ; Anhui Provincial Undergraduate Innovation and Entrepreneurship Program(201510363130) ; Anhui Provincial Undergraduate Innovation and Entrepreneurship Program(201510363130) ; Anhui Provincial Undergraduate Innovation and Entrepreneurship Program(201510363130) ; National Undergraduate Innovation and Entrepreneurship Program(2016103630055) ; National Undergraduate Innovation and Entrepreneurship Program(2016103630055) ; National Undergraduate Innovation and Entrepreneurship Program(2016103630055) ; National Undergraduate Innovation and Entrepreneurship Program(2016103630055) ; 31471615) ; 31471615) ; 31471615) ; 31471615)
WOS研究方向	Biotechnology & Applied Microbiology
WOS类目	Biotechnology & Applied Microbiology
WOS记录号	WOS:000398100300011
引用统计	被引频次：14[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.hfcas.ac.cn:8080/handle/334002/32899
专题	技术生物与农业工程研究所
作者单位	1.Anhui Polytech Univ, Coll Biochem Engn, Wuhu 241000, Peoples R China 2.Chinese Acad Sci, Hefei Inst Phys Sci, Key Lab Ion Beam Bioengn, Hefei 230031, Peoples R China 3.Virginia Commonwealth Univ, Sch Med, VCU Massey Canc Ctr, VCU Inst Mol Genet, Med Coll Virginia Campus, Richmond, VA 23298 USA
推荐引用方式 GB/T 7714	Liu, Yan,Ding, Xiu-min,Xue, Zheng-lian,et al. The change of the state of cell membrane can enhance the synthesis of menaquinone in Escherichia coli[J]. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,2017,33(3):1-11.
APA	Liu, Yan.,Ding, Xiu-min.,Xue, Zheng-lian.,Hu, Liu-xiu.,Zhang, Ning-juan.,...&Zheng, Zhi-ming.(2017).The change of the state of cell membrane can enhance the synthesis of menaquinone in Escherichia coli.WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,33(3),1-11.
MLA	Liu, Yan,et al."The change of the state of cell membrane can enhance the synthesis of menaquinone in Escherichia coli".WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 33.3(2017):1-11.

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