Institutional Repository of Chinese Acad Sci, High Field Magnet Lab,Hefei 230031, Anhui, Peoples R China
| Temperature-dependent ESR and computational studies on antiferromagnetic electron transfer in the yeast NADH dehydrogenase Ndi1 | |
Wu, Kaiqi1; Li, Wenfei2; Yu, Lu3; Tong, Wei3 ; Feng, Yue2; Ling, Shenglong1; Zhang, Longhua1; Zheng, Xiao1; Yang, Maojun2; Tian, Changlin1,3
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| 2017-02-14 | |
| 发表期刊 | PHYSICAL CHEMISTRY CHEMICAL PHYSICS
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| 摘要 | Ndi1 is a special type-II complex I nicotinamide-adenine-dinucleotide (NADH): ubiquinone (UQ) oxidoreductase in the yeast respiratory chain, with two bound UQs (UQI and UQII) mediating electron transfer from flavin cofactors to ubiquinone, in the absence of Fe-S chains. Here, we elucidate the underlying mechanism of electron transfer in Ndi1 through temperature-dependent Electron Spin Resonance (ESR) experiments in conjunction with quantum chemical calculations. It is revealed that electron transfer is mediated by antiferromagnetic (AFM) interactions between flavin-adenosine-dinucleotide (FAD) and UQI and between UQI and UQII. The p-stacking interactions among the aromatic complexes also enhance the throughspace electron transfer. The FAD/UQI pair works as a rectifier converting double-electron co-transfer into sequential single-electron transfer events. The results not only expand our understanding on the observed AFM interactions among p-orbital aromatic mixed-stack in proteins, but also provide significant insights into the fabrication of materials with special magnetic properties using biological samples. |
| 文章类型 | Article |
| WOS标题词 | Science & Technology ; Physical Sciences |
| DOI | 10.1039/c6cp08107j |
| 关键词[WOS] | RESPIRATORY COMPLEX-I ; BACTERIAL REACTION CENTERS ; EXCHANGE ; DENSITY ; EPR ; OXIDOREDUCTASE ; PROTEOLIPOSOMES ; UBISEMIQUINONE ; STABILIZATION ; CLUSTERS |
| 收录类别 | SCI |
| 语种 | 英语 |
| 项目资助者 | Ministry of Science and Technology of the People's Republic of China(2011CB911104 ; Ministry of Science and Technology of the People's Republic of China(2011CB911104 ; Ministry of Science and Technology of the People's Republic of China(2011CB911104 ; Ministry of Science and Technology of the People's Republic of China(2011CB911104 ; Ministry of Science and Technology of the People's Republic of China(2011CB911104 ; Ministry of Science and Technology of the People's Republic of China(2011CB911104 ; Ministry of Science and Technology of the People's Republic of China(2011CB911104 ; Ministry of Science and Technology of the People's Republic of China(2011CB911104 ; National Natural Science Foundation of China(31500611) ; National Natural Science Foundation of China(31500611) ; National Natural Science Foundation of China(31500611) ; National Natural Science Foundation of China(31500611) ; National Natural Science Foundation of China(31500611) ; National Natural Science Foundation of China(31500611) ; National Natural Science Foundation of China(31500611) ; National Natural Science Foundation of China(31500611) ; 2015CB910103 ; 2015CB910103 ; 2015CB910103 ; 2015CB910103 ; 2015CB910103 ; 2015CB910103 ; 2015CB910103 ; 2015CB910103 ; 2011CB910502 ; 2011CB910502 ; 2011CB910502 ; 2011CB910502 ; 2011CB910502 ; 2011CB910502 ; 2011CB910502 ; 2011CB910502 ; 2012CB911101) ; 2012CB911101) ; 2012CB911101) ; 2012CB911101) ; 2012CB911101) ; 2012CB911101) ; 2012CB911101) ; 2012CB911101) |
| WOS研究方向 | Chemistry ; Physics |
| WOS类目 | Chemistry, Physical ; Physics, Atomic, Molecular & Chemical |
| WOS记录号 | WOS:000395526600077 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.hfcas.ac.cn:8080/handle/334002/32920 |
| 专题 | 中科院强磁场科学中心 |
| 作者单位 | 1.Univ Sci & Technol China, Hefei Natl Lab Phys Sci Microscale, Sch Life Sci, Hefei 230026, Peoples R China 2.Tsinghua Univ, State Key Lab Biomembrane & Membrane Biotechnol, Tsinghua Peking Ctr Life Sci, Sch Life Sci, Beijing 100084, Peoples R China 3.Chinese Acad Sci, High Field Magnet Lab, Hefei 230031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, Kaiqi,Li, Wenfei,Yu, Lu,et al. Temperature-dependent ESR and computational studies on antiferromagnetic electron transfer in the yeast NADH dehydrogenase Ndi1[J]. PHYSICAL CHEMISTRY CHEMICAL PHYSICS,2017,19(6):4849-4854. |
| APA | Wu, Kaiqi.,Li, Wenfei.,Yu, Lu.,Tong, Wei.,Feng, Yue.,...&Tian, Changlin.(2017).Temperature-dependent ESR and computational studies on antiferromagnetic electron transfer in the yeast NADH dehydrogenase Ndi1.PHYSICAL CHEMISTRY CHEMICAL PHYSICS,19(6),4849-4854. |
| MLA | Wu, Kaiqi,et al."Temperature-dependent ESR and computational studies on antiferromagnetic electron transfer in the yeast NADH dehydrogenase Ndi1".PHYSICAL CHEMISTRY CHEMICAL PHYSICS 19.6(2017):4849-4854. |
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