Nrf-2-driven long noncoding RNA ODRUL contributes to modulating silver nanoparticle-induced effects on erythroid cells

doi:10.1016/j.biomaterials.2017.03.027

	Nrf-2-driven long noncoding RNA ODRUL contributes to modulating silver nanoparticle-induced effects on erythroid cells
	Gao, Ming 1; Zhao, Beibei 2; Chen, Minjun 3; Liu, Yun1,4 ; Xu, Ming1 ; Wang, Zhe 5; Liu, Sijin 1; Zhang, Chengdong 2
	2017-06-01
发表期刊	BIOMATERIALS
摘要	The biosafety and biological effects of silver nanoparticles (AgNPs) on human health attract increasing concern. Although considerable studies have been performed to reveal the molecular mechanisms responsible for AgNP-induced effects, the current understanding mainly focuses on oxidative stress-associated signaling pathways activated by Ag particles and/or Ag ions. However, the molecular bases underlying the activation of these stress signaling pathways have not been thoroughly elucidated yet. In the current study, we aimed to shed light on the molecular bases of AgNP-induced effects on erythroid cells from the perspective of long noncoding RNAs. We identified a long-noncoding RNA molecule, ODRUL, which was substantially enhanced in K562 erythroid cells responding to AgNPs, coupled to accelerated cell death. Further, we uncovered oxidative stress-driven Nrf2 transcriptionally promoted ODRUL expression in K562 cells. Downstream of Nrf2-ODRUL activation by AgNPs, ODRUL was recognized to interact with PI4Ka protein to modulate the activities of its targets AKT and JNK. As a result, the Bcl-2 level was negatively regulated by PI4K-AKT/JNK signaling under AgNP-induced stress, leading to enhanced cell death. Together, our findings unearthed that Nrf2-mediated lncRNA ODRUL was indispensable for AgNP-induced toxicity in erythroid cells through regulation of AKT/JNK-Bcl-2 signaling dependent on a physical interaction with PI4Ka. Thus, this study would open a new path to depict the molecular bases of AgNP-induced effects on erythroid cells. (C) 2017 Elsevier Ltd. All rights reserved.
文章类型	Article
关键词	Silver Nanoparticles Lncrna Odrul Cell Death Erythroid Cells
WOS标题词	Science & Technology ; Technology
DOI	10.1016/j.biomaterials.2017.03.027
关键词[WOS]	OXIDATIVE STRESS ; GENE-EXPRESSION ; LEUKEMIA-CELLS ; ENGINEERED NANOMATERIALS ; INDUCED-DIFFERENTIATION ; GRAPHENE OXIDE ; IN-VITRO ; APOPTOSIS ; CANCER ; TOXICITY
收录类别	SCI
语种	英语
项目资助者	National Natural Science Foundation of China(21507154 ; National Natural Science Foundation of China(21507154 ; National Natural Science Foundation of China(21507154 ; National Natural Science Foundation of China(21507154 ; National Natural Science Foundation of China(21507154 ; National Natural Science Foundation of China(21507154 ; National Natural Science Foundation of China(21507154 ; National Natural Science Foundation of China(21507154 ; national "973" program(2014CB932000) ; national "973" program(2014CB932000) ; national "973" program(2014CB932000) ; national "973" program(2014CB932000) ; national "973" program(2014CB932000) ; national "973" program(2014CB932000) ; national "973" program(2014CB932000) ; national "973" program(2014CB932000) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDB14000000) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDB14000000) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDB14000000) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDB14000000) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDB14000000) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDB14000000) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDB14000000) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDB14000000) ; 21407172 ; 21407172 ; 21407172 ; 21407172 ; 21407172 ; 21407172 ; 21407172 ; 21407172 ; 21425731 ; 21425731 ; 21425731 ; 21425731 ; 21425731 ; 21425731 ; 21425731 ; 21425731 ; 21637004 ; 21637004 ; 21637004 ; 21637004 ; 21637004 ; 21637004 ; 21637004 ; 21637004 ; 81373039) ; 81373039) ; 81373039) ; 81373039) ; 81373039) ; 81373039) ; 81373039) ; 81373039)
WOS研究方向	Engineering ; Materials Science
WOS类目	Engineering, Biomedical ; Materials Science, Biomaterials
WOS记录号	WOS:000399432300002
引用统计	被引频次：37[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.hfcas.ac.cn:8080/handle/334002/33312
专题	技术生物与农业工程研究所
作者单位	1.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China 2.Nankai Univ, Tianjin Key Lab Environm Remediat & Pollut Contro, Key Lab Pollut Proc & Environm Criteria, Minist Educ,Coll Environm Sci & Engn, Tianjin, Peoples R China 3.Shanxi Univ, Coll Environm & Resource, Res Ctr Environm & Hlth, Taiyuan 030006, Shanxi, Peoples R China 4.Chinese Acad Sci & Anhui Prov, Hefei Inst Phys Sci, Key Lab Ion Beam Bioengn, Hefei 230031, Anhui, Peoples R China 5.Xinxiang Med Univ, Sch Publ Hlth, Xinxiang 453003, Henan Province, Peoples R China
推荐引用方式 GB/T 7714	Gao, Ming,Zhao, Beibei,Chen, Minjun,et al. Nrf-2-driven long noncoding RNA ODRUL contributes to modulating silver nanoparticle-induced effects on erythroid cells[J]. BIOMATERIALS,2017,130:14-27.
APA	Gao, Ming.,Zhao, Beibei.,Chen, Minjun.,Liu, Yun.,Xu, Ming.,...&Zhang, Chengdong.(2017).Nrf-2-driven long noncoding RNA ODRUL contributes to modulating silver nanoparticle-induced effects on erythroid cells.BIOMATERIALS,130,14-27.
MLA	Gao, Ming,et al."Nrf-2-driven long noncoding RNA ODRUL contributes to modulating silver nanoparticle-induced effects on erythroid cells".BIOMATERIALS 130(2017):14-27.