Institutional Repository of Key Laboratory of Ion Beam Bioengineering, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, Anhui 230031, People's Republic of China
Uptake of silver nanoparticles by DHA-treated cancer cells examined by surface-enhanced Raman spectroscopy in a microfluidic chip | |
Zhai, Zhimin1,2; Zhang, Fengqiu1,2,3; Chen, Xiangyu2; Zhong, Jie1,2; Liu, Gang2; Tian, Yangchao2; Huang, Qing1,2 | |
2017-04-07 | |
发表期刊 | LAB ON A CHIP |
摘要 | This paper reports on the synthesis and application of biocompatible and sensitive SERS nanoparticles for the study of uptake of nanoparticles into living cells in a microfluidic chip through surface-enhanced Raman spectroscopy (SERS). The nanoparticles were fabricated as beta-cyclodextrin-coated silver nanoparticles (Ag@CD NPs) modified with para-aminothiophenol (p-ATP) and folic acid (FA) on the surface. The p-ATP molecules act as the Raman reporter while the FA tags have high affinity for folate receptors (FR) that are over-expressed on the surface cancerous cells, so that the nanoparticles can enter the cells and be monitored by the Raman reporter. Therefore, the nanoparticles could be utilized not only as cell invaders due to endocytosis but also as a SERS sensitive probe to monitor the effect of FR-targeted drugs such as dihydroartemisinin (DHA) that induce the population change of FR on the membrane of living cells. As a result, we have successfully demonstrated that we are able to employ the Ag@CD@p-ATP@FA NPs to evaluate the number of NPs entering living cells quantitatively and correspondingly the drug effect on cancer cells in a well-controlled way. |
文章类型 | Article |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
DOI | 10.1039/c7lc00053g |
关键词[WOS] | LABEL-FREE DETECTION ; FOLATE RECEPTOR EXPRESSION ; GOLD NANOPARTICLES ; FOLIC-ACID ; SCATTERING SPECTROSCOPY ; MOLECULAR RECOGNITION ; SERS NANOPARTICLES ; OVARIAN-CANCER ; SINGLE-CELL ; IN-VIVO |
收录类别 | SCI |
语种 | 英语 |
项目资助者 | National Natural Science Foundation of China(11635013) ; National Natural Science Foundation of China(11635013) ; National Natural Science Foundation of China(11635013) ; National Natural Science Foundation of China(11635013) ; National Natural Science Foundation of China(11635013) ; National Natural Science Foundation of China(11635013) ; National Natural Science Foundation of China(11635013) ; National Natural Science Foundation of China(11635013) ; National Basic Research Program of China(2013CB934304 ; National Basic Research Program of China(2013CB934304 ; National Basic Research Program of China(2013CB934304 ; National Basic Research Program of China(2013CB934304 ; National Basic Research Program of China(2013CB934304 ; National Basic Research Program of China(2013CB934304 ; National Basic Research Program of China(2013CB934304 ; National Basic Research Program of China(2013CB934304 ; 2014CB932002) ; 2014CB932002) ; 2014CB932002) ; 2014CB932002) ; 2014CB932002) ; 2014CB932002) ; 2014CB932002) ; 2014CB932002) |
WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry ; Science & Technology - Other Topics |
WOS类目 | Biochemical Research Methods ; Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology |
WOS记录号 | WOS:000398955800010 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.hfcas.ac.cn:8080/handle/334002/33315 |
专题 | 技术生物与农业工程研究所 |
作者单位 | 1.Chinese Acad Sci, Hefei Inst Phys Sci, Inst Tech Biol & Agr Engn, Hefei 230031, Peoples R China 2.Univ Sci & Technol China, Hefei 230026, Peoples R China 3.Zhengzhou Univ, Sch Phys Engn, Zhengzhou, Peoples R China |
推荐引用方式 GB/T 7714 | Zhai, Zhimin,Zhang, Fengqiu,Chen, Xiangyu,et al. Uptake of silver nanoparticles by DHA-treated cancer cells examined by surface-enhanced Raman spectroscopy in a microfluidic chip[J]. LAB ON A CHIP,2017,17(7):1306-1313. |
APA | Zhai, Zhimin.,Zhang, Fengqiu.,Chen, Xiangyu.,Zhong, Jie.,Liu, Gang.,...&Huang, Qing.(2017).Uptake of silver nanoparticles by DHA-treated cancer cells examined by surface-enhanced Raman spectroscopy in a microfluidic chip.LAB ON A CHIP,17(7),1306-1313. |
MLA | Zhai, Zhimin,et al."Uptake of silver nanoparticles by DHA-treated cancer cells examined by surface-enhanced Raman spectroscopy in a microfluidic chip".LAB ON A CHIP 17.7(2017):1306-1313. |
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