Uptake of silver nanoparticles by DHA-treated cancer cells examined by surface-enhanced Raman spectroscopy in a microfluidic chip

doi:10.1039/c7lc00053g

	Uptake of silver nanoparticles by DHA-treated cancer cells examined by surface-enhanced Raman spectroscopy in a microfluidic chip
	Zhai, Zhimin 1,2; Zhang, Fengqiu 1,2,3; Chen, Xiangyu2 ; Zhong, Jie 1,2; Liu, Gang2 ; Tian, Yangchao 2; Huang, Qing1,2
	2017-04-07
发表期刊	LAB ON A CHIP
摘要	This paper reports on the synthesis and application of biocompatible and sensitive SERS nanoparticles for the study of uptake of nanoparticles into living cells in a microfluidic chip through surface-enhanced Raman spectroscopy (SERS). The nanoparticles were fabricated as beta-cyclodextrin-coated silver nanoparticles (Ag@CD NPs) modified with para-aminothiophenol (p-ATP) and folic acid (FA) on the surface. The p-ATP molecules act as the Raman reporter while the FA tags have high affinity for folate receptors (FR) that are over-expressed on the surface cancerous cells, so that the nanoparticles can enter the cells and be monitored by the Raman reporter. Therefore, the nanoparticles could be utilized not only as cell invaders due to endocytosis but also as a SERS sensitive probe to monitor the effect of FR-targeted drugs such as dihydroartemisinin (DHA) that induce the population change of FR on the membrane of living cells. As a result, we have successfully demonstrated that we are able to employ the Ag@CD@p-ATP@FA NPs to evaluate the number of NPs entering living cells quantitatively and correspondingly the drug effect on cancer cells in a well-controlled way.
文章类型	Article
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI	10.1039/c7lc00053g
关键词[WOS]	LABEL-FREE DETECTION ; FOLATE RECEPTOR EXPRESSION ; GOLD NANOPARTICLES ; FOLIC-ACID ; SCATTERING SPECTROSCOPY ; MOLECULAR RECOGNITION ; SERS NANOPARTICLES ; OVARIAN-CANCER ; SINGLE-CELL ; IN-VIVO
收录类别	SCI
语种	英语
项目资助者	National Natural Science Foundation of China(11635013) ; National Natural Science Foundation of China(11635013) ; National Natural Science Foundation of China(11635013) ; National Natural Science Foundation of China(11635013) ; National Natural Science Foundation of China(11635013) ; National Natural Science Foundation of China(11635013) ; National Natural Science Foundation of China(11635013) ; National Natural Science Foundation of China(11635013) ; National Basic Research Program of China(2013CB934304 ; National Basic Research Program of China(2013CB934304 ; National Basic Research Program of China(2013CB934304 ; National Basic Research Program of China(2013CB934304 ; National Basic Research Program of China(2013CB934304 ; National Basic Research Program of China(2013CB934304 ; National Basic Research Program of China(2013CB934304 ; National Basic Research Program of China(2013CB934304 ; 2014CB932002) ; 2014CB932002) ; 2014CB932002) ; 2014CB932002) ; 2014CB932002) ; 2014CB932002) ; 2014CB932002) ; 2014CB932002)
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry ; Science & Technology - Other Topics
WOS类目	Biochemical Research Methods ; Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology
WOS记录号	WOS:000398955800010
引用统计	被引频次：26[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.hfcas.ac.cn:8080/handle/334002/33385
专题	技术生物与农业工程研究所
作者单位	1.Chinese Acad Sci, Hefei Inst Phys Sci, Inst Tech Biol & Agr Engn, Hefei 230031, Peoples R China 2.Univ Sci & Technol China, Hefei 230026, Peoples R China 3.Zhengzhou Univ, Sch Phys Engn, Zhengzhou, Peoples R China
推荐引用方式 GB/T 7714	Zhai, Zhimin,Zhang, Fengqiu,Chen, Xiangyu,et al. Uptake of silver nanoparticles by DHA-treated cancer cells examined by surface-enhanced Raman spectroscopy in a microfluidic chip[J]. LAB ON A CHIP,2017,17(7):1306-1313.
APA	Zhai, Zhimin.,Zhang, Fengqiu.,Chen, Xiangyu.,Zhong, Jie.,Liu, Gang.,...&Huang, Qing.(2017).Uptake of silver nanoparticles by DHA-treated cancer cells examined by surface-enhanced Raman spectroscopy in a microfluidic chip.LAB ON A CHIP,17(7),1306-1313.
MLA	Zhai, Zhimin,et al."Uptake of silver nanoparticles by DHA-treated cancer cells examined by surface-enhanced Raman spectroscopy in a microfluidic chip".LAB ON A CHIP 17.7(2017):1306-1313.