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Uptake of silver nanoparticles by DHA-treated cancer cells examined by surface-enhanced Raman spectroscopy in a microfluidic chip
Zhai, Zhimin1,2; Zhang, Fengqiu1,2,3; Chen, Xiangyu2; Zhong, Jie1,2; Liu, Gang2; Tian, Yangchao2; Huang, Qing1,2
2017-04-07
Source PublicationLAB ON A CHIP
Volume17Issue:7Pages:1306-1313
AbstractThis paper reports on the synthesis and application of biocompatible and sensitive SERS nanoparticles for the study of uptake of nanoparticles into living cells in a microfluidic chip through surface-enhanced Raman spectroscopy (SERS). The nanoparticles were fabricated as beta-cyclodextrin-coated silver nanoparticles (Ag@CD NPs) modified with para-aminothiophenol (p-ATP) and folic acid (FA) on the surface. The p-ATP molecules act as the Raman reporter while the FA tags have high affinity for folate receptors (FR) that are over-expressed on the surface cancerous cells, so that the nanoparticles can enter the cells and be monitored by the Raman reporter. Therefore, the nanoparticles could be utilized not only as cell invaders due to endocytosis but also as a SERS sensitive probe to monitor the effect of FR-targeted drugs such as dihydroartemisinin (DHA) that induce the population change of FR on the membrane of living cells. As a result, we have successfully demonstrated that we are able to employ the Ag@CD@p-ATP@FA NPs to evaluate the number of NPs entering living cells quantitatively and correspondingly the drug effect on cancer cells in a well-controlled way.
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
Funding OrganizationNational Natural Science Foundation of China(11635013) ; National Natural Science Foundation of China(11635013) ; National Natural Science Foundation of China(11635013) ; National Natural Science Foundation of China(11635013) ; National Natural Science Foundation of China(11635013) ; National Natural Science Foundation of China(11635013) ; National Natural Science Foundation of China(11635013) ; National Natural Science Foundation of China(11635013) ; National Basic Research Program of China(2013CB934304 ; National Basic Research Program of China(2013CB934304 ; National Basic Research Program of China(2013CB934304 ; National Basic Research Program of China(2013CB934304 ; National Basic Research Program of China(2013CB934304 ; National Basic Research Program of China(2013CB934304 ; National Basic Research Program of China(2013CB934304 ; National Basic Research Program of China(2013CB934304 ; 2014CB932002) ; 2014CB932002) ; 2014CB932002) ; 2014CB932002) ; 2014CB932002) ; 2014CB932002) ; 2014CB932002) ; 2014CB932002) ; National Natural Science Foundation of China(11635013) ; National Natural Science Foundation of China(11635013) ; National Natural Science Foundation of China(11635013) ; National Natural Science Foundation of China(11635013) ; National Natural Science Foundation of China(11635013) ; National Natural Science Foundation of China(11635013) ; National Natural Science Foundation of China(11635013) ; National Natural Science Foundation of China(11635013) ; National Basic Research Program of China(2013CB934304 ; National Basic Research Program of China(2013CB934304 ; National Basic Research Program of China(2013CB934304 ; National Basic Research Program of China(2013CB934304 ; National Basic Research Program of China(2013CB934304 ; National Basic Research Program of China(2013CB934304 ; National Basic Research Program of China(2013CB934304 ; National Basic Research Program of China(2013CB934304 ; 2014CB932002) ; 2014CB932002) ; 2014CB932002) ; 2014CB932002) ; 2014CB932002) ; 2014CB932002) ; 2014CB932002) ; 2014CB932002)
DOI10.1039/c7lc00053g
WOS KeywordLABEL-FREE DETECTION ; FOLATE RECEPTOR EXPRESSION ; GOLD NANOPARTICLES ; FOLIC-ACID ; SCATTERING SPECTROSCOPY ; MOLECULAR RECOGNITION ; SERS NANOPARTICLES ; OVARIAN-CANCER ; SINGLE-CELL ; IN-VIVO
Indexed BySCI
Language英语
Funding OrganizationNational Natural Science Foundation of China(11635013) ; National Natural Science Foundation of China(11635013) ; National Natural Science Foundation of China(11635013) ; National Natural Science Foundation of China(11635013) ; National Natural Science Foundation of China(11635013) ; National Natural Science Foundation of China(11635013) ; National Natural Science Foundation of China(11635013) ; National Natural Science Foundation of China(11635013) ; National Basic Research Program of China(2013CB934304 ; National Basic Research Program of China(2013CB934304 ; National Basic Research Program of China(2013CB934304 ; National Basic Research Program of China(2013CB934304 ; National Basic Research Program of China(2013CB934304 ; National Basic Research Program of China(2013CB934304 ; National Basic Research Program of China(2013CB934304 ; National Basic Research Program of China(2013CB934304 ; 2014CB932002) ; 2014CB932002) ; 2014CB932002) ; 2014CB932002) ; 2014CB932002) ; 2014CB932002) ; 2014CB932002) ; 2014CB932002) ; National Natural Science Foundation of China(11635013) ; National Natural Science Foundation of China(11635013) ; National Natural Science Foundation of China(11635013) ; National Natural Science Foundation of China(11635013) ; National Natural Science Foundation of China(11635013) ; National Natural Science Foundation of China(11635013) ; National Natural Science Foundation of China(11635013) ; National Natural Science Foundation of China(11635013) ; National Basic Research Program of China(2013CB934304 ; National Basic Research Program of China(2013CB934304 ; National Basic Research Program of China(2013CB934304 ; National Basic Research Program of China(2013CB934304 ; National Basic Research Program of China(2013CB934304 ; National Basic Research Program of China(2013CB934304 ; National Basic Research Program of China(2013CB934304 ; National Basic Research Program of China(2013CB934304 ; 2014CB932002) ; 2014CB932002) ; 2014CB932002) ; 2014CB932002) ; 2014CB932002) ; 2014CB932002) ; 2014CB932002) ; 2014CB932002)
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry ; Science & Technology - Other Topics
WOS SubjectBiochemical Research Methods ; Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology
WOS IDWOS:000398955800010
Citation statistics
Cited Times:11[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.hfcas.ac.cn:8080/handle/334002/33385
Collection技术生物与农业工程研究所
Affiliation1.Chinese Acad Sci, Hefei Inst Phys Sci, Inst Tech Biol & Agr Engn, Hefei 230031, Peoples R China
2.Univ Sci & Technol China, Hefei 230026, Peoples R China
3.Zhengzhou Univ, Sch Phys Engn, Zhengzhou, Peoples R China
Recommended Citation
GB/T 7714
Zhai, Zhimin,Zhang, Fengqiu,Chen, Xiangyu,et al. Uptake of silver nanoparticles by DHA-treated cancer cells examined by surface-enhanced Raman spectroscopy in a microfluidic chip[J]. LAB ON A CHIP,2017,17(7):1306-1313.
APA Zhai, Zhimin.,Zhang, Fengqiu.,Chen, Xiangyu.,Zhong, Jie.,Liu, Gang.,...&Huang, Qing.(2017).Uptake of silver nanoparticles by DHA-treated cancer cells examined by surface-enhanced Raman spectroscopy in a microfluidic chip.LAB ON A CHIP,17(7),1306-1313.
MLA Zhai, Zhimin,et al."Uptake of silver nanoparticles by DHA-treated cancer cells examined by surface-enhanced Raman spectroscopy in a microfluidic chip".LAB ON A CHIP 17.7(2017):1306-1313.
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