Mitochondria and MAPK cascades modulate endosulfan-induced germline apoptosis in Caenorhabditis elegans

doi:10.1039/c7tx00046d

	Mitochondria and MAPK cascades modulate endosulfan-induced germline apoptosis in Caenorhabditis elegans
	Wang, Jingjing1,2 ; Du, Hua2 ; Nie, Yaguang 2; Wang, Yun3 ; Dai, Hui 2; Wang, Mudi 2; Wang, Dayan 1,2; Xu, An2
	2017-07-01
发表期刊	TOXICOLOGY RESEARCH
摘要	Endosulfan as a new member of persistent organic pollutants has been shown to induce apoptosis in various animal models. However, the mechanism underlying endosulfan-induced apoptosis has not been well elucidated thus far. Caenorhabditis elegans N2 wild type and mutant strains were used in the present study to clarify the roles of the mitochondria, the insulin/insulin-like growth factor-1 (IGF-1) signaling pathway, and mitogen-activated protein kinase (MAPK) cascades in alpha-endosulfan-induced apoptosis. Our results demonstrated a dose- and time-dependent increase of apoptosis in the meiotic zone of the gonad of C. elegans exposed to graded concentrations of endosulfan. The expression levels of sod-3, localized in the mitochondrial matrix, increased greatly after endosulfan exposure. A significant increase in germ cell apoptosis was observed in abnormal methyl viologen sensitivity-1 (mev-1(kn-1)) mutants (with abnormal mitochondrial respiratory chain complex II and higher ROS levels) compared to that in N2 at equal endosulfan concentrations. We found that the insulin/IGF-1 signaling pathway and its downstream Ras/ERK/MAPK did not participate in the endosulfan-induced apoptosis. However, the apoptosis in the loss-of-function strains of JNK and p38 MAPK signaling pathways was completely or mildly suppressed under endosulfan stress. The apoptotic effects of endosulfan were blocked in the mutants of jnk-1/JNK-MAPK, sek-1/MAP2K, and pmk-1/p38-MAPK, suggesting that these downstream genes play an essential role in endosulfan-induced germ cell apoptosis. In contrast, the mkk-4/MAP2K and nsy-1/MAP3K were only partially involved in the apoptosis induction. Our data provide evidence that endosulfan increases germ cell apoptosis, which is regulated by mitochondrial function, JNK and p38 MAPK cascades. These findings contribute to the understanding of the signal transduction pathways involved in endosulfan-induced apoptosis.
文章类型	Article
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1039/c7tx00046d
关键词[WOS]	GENOTOXIC RESPONSE GENES ; GRAPHENE OXIDE TOXICITY ; SIGNALING PATHWAYS ; CELL-DEATH ; REPRODUCTIVE TOXICITY ; OXIDATIVE STRESS ; REACTIVE OXYGEN ; INSULIN ACTION ; ACTIVATION ; ERK
收录类别	SCI
语种	英语
项目资助者	Major National Scientific Research Projects(2014CB932002) ; Major National Scientific Research Projects(2014CB932002) ; Major National Scientific Research Projects(2014CB932002) ; Major National Scientific Research Projects(2014CB932002) ; Major National Scientific Research Projects(2014CB932002) ; Major National Scientific Research Projects(2014CB932002) ; Major National Scientific Research Projects(2014CB932002) ; Major National Scientific Research Projects(2014CB932002) ; Strategic Leading Science & Technology Program (B)(XDB14030502) ; Strategic Leading Science & Technology Program (B)(XDB14030502) ; Strategic Leading Science & Technology Program (B)(XDB14030502) ; Strategic Leading Science & Technology Program (B)(XDB14030502) ; Strategic Leading Science & Technology Program (B)(XDB14030502) ; Strategic Leading Science & Technology Program (B)(XDB14030502) ; Strategic Leading Science & Technology Program (B)(XDB14030502) ; Strategic Leading Science & Technology Program (B)(XDB14030502) ; National Natural Science Foundation of China(21677147 ; National Natural Science Foundation of China(21677147 ; National Natural Science Foundation of China(21677147 ; National Natural Science Foundation of China(21677147 ; National Natural Science Foundation of China(21677147 ; National Natural Science Foundation of China(21677147 ; National Natural Science Foundation of China(21677147 ; National Natural Science Foundation of China(21677147 ; China Postdoctoral Science Foundation(2015 M82023 ; China Postdoctoral Science Foundation(2015 M82023 ; China Postdoctoral Science Foundation(2015 M82023 ; China Postdoctoral Science Foundation(2015 M82023 ; China Postdoctoral Science Foundation(2015 M82023 ; China Postdoctoral Science Foundation(2015 M82023 ; China Postdoctoral Science Foundation(2015 M82023 ; China Postdoctoral Science Foundation(2015 M82023 ; CPSF-CAS Joint Foundation(2015LH0017) ; CPSF-CAS Joint Foundation(2015LH0017) ; CPSF-CAS Joint Foundation(2015LH0017) ; CPSF-CAS Joint Foundation(2015LH0017) ; CPSF-CAS Joint Foundation(2015LH0017) ; CPSF-CAS Joint Foundation(2015LH0017) ; CPSF-CAS Joint Foundation(2015LH0017) ; CPSF-CAS Joint Foundation(2015LH0017) ; CASHIPS director's fund(YZJJ201501) ; CASHIPS director's fund(YZJJ201501) ; CASHIPS director's fund(YZJJ201501) ; CASHIPS director's fund(YZJJ201501) ; CASHIPS director's fund(YZJJ201501) ; CASHIPS director's fund(YZJJ201501) ; CASHIPS director's fund(YZJJ201501) ; CASHIPS director's fund(YZJJ201501) ; 21507136) ; 21507136) ; 21507136) ; 21507136) ; 21507136) ; 21507136) ; 21507136) ; 21507136) ; 2015 M582006) ; 2015 M582006) ; 2015 M582006) ; 2015 M582006) ; 2015 M582006) ; 2015 M582006) ; 2015 M582006) ; 2015 M582006)
WOS研究方向	Toxicology
WOS类目	Toxicology
WOS记录号	WOS:000404873500003
引用统计	被引频次：15[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.hfcas.ac.cn:8080/handle/334002/33467
专题	技术生物与农业工程研究所
作者单位	1.Univ Sci & Technol China, Sch Environm Sci & Optoelect Technol, Hefei 230026, Anhui, Peoples R China 2.Chinese Acad Sci & Anhui Prov, Hefei Inst Phys Sci, Key Lab Ion Beam Bioengn, Hefei 230031, Anhui, Peoples R China 3.Univ Sci & Technol China, Sch Life Sci, Hefei 230027, Anhui, Peoples R China
推荐引用方式 GB/T 7714	Wang, Jingjing,Du, Hua,Nie, Yaguang,et al. Mitochondria and MAPK cascades modulate endosulfan-induced germline apoptosis in Caenorhabditis elegans[J]. TOXICOLOGY RESEARCH,2017,6(4):412-419.
APA	Wang, Jingjing.,Du, Hua.,Nie, Yaguang.,Wang, Yun.,Dai, Hui.,...&Xu, An.(2017).Mitochondria and MAPK cascades modulate endosulfan-induced germline apoptosis in Caenorhabditis elegans.TOXICOLOGY RESEARCH,6(4),412-419.
MLA	Wang, Jingjing,et al."Mitochondria and MAPK cascades modulate endosulfan-induced germline apoptosis in Caenorhabditis elegans".TOXICOLOGY RESEARCH 6.4(2017):412-419.