Research on Fluorescence Spectral Characteristics of Phycocyanin under Different Habitat Conditions

doi:10.3964/j.issn.1000-0593(2017)04-1145-07

	Research on Fluorescence Spectral Characteristics of Phycocyanin under Different Habitat Conditions
	Zhang Xiao-Ling 1,2,3; Yin Gao-Fang 1,2,3; Zhao Nan-Jing1,2,3 ; Qin Zhi-Song 1,2,3; Xiao Xuel 1,2,3; Duan Jing-bo 1,2,3; Yang Rui-Fang 1,2,3; Liu Jian-Guo1,2,3 ; Liu Wen-Qing1,2,3
	2017-04-01
发表期刊	SPECTROSCOPY AND SPECTRAL ANALYSIS
摘要	The development of cyanobacterial biomass detection technology is an important part to deal with the frequent occurrence of water bloom events. Phycocyanin, to some extent, as a special protein of cyanobacteria, is more accurate reaction in natural water cyanobacterial biomassthan chlorophyll, thus become an important index in cyanobacterial biomass detection. This paper, with different light intensity and different growth stages as the research objects. compares the reliability of the two methods of the envelope method and the single point method, exploring the fluorescence spectral characteristics of phycocyanin in vivo Microcystis Anabaena by using three-dimensional fluorescence spectroscopy. The results show that: (1) The fluorescence intensity increases with the growth of long-term; (2) It is more reliable to use envelope method to analyze the characteristic fluorescence spectra of the fluorescent spectrum than the single point method. (3) The fluorescence spectra of Phycocyanin in vivo Microcystis were basically unchanged in EX=614 nm/ EM=654 nm. The excitation wavelength of the phycocyanin fluorescence in vivo Microcystis decreases with the growth with 610 and 620 nm in the long term, and the emission wavelength varied between 650 and 660 nm, which is related to the sample particle size and spectral scanning mode. This study provided the experimental basis for the development of the fluorescent detection technology of cyanobacteria.
文章类型	Article
关键词	Phycocyanin In Vivo Fluorescence Cyanobacteria Phytoplankton
WOS标题词	Science & Technology ; Technology
DOI	10.3964/j.issn.1000-0593(2017)04-1145-07
收录类别	SCI
语种	英语
WOS研究方向	Spectroscopy
WOS类目	Spectroscopy
WOS记录号	WOS:000400041300029
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.hfcas.ac.cn:8080/handle/334002/33505
专题	中科院安徽光学精密机械研究所
作者单位	1.Chinese Acad Sci, Anhui Inst Opt & Fine Mech, Key Lab Environm Opt & Technol, Hefei 230031, Peoples R China 2.Univ Sci & Technol China, Hefei 230036, Peoples R China 3.Key Lab Opt Monitoring Technol Environm, Hefei 230031, Anhui, Peoples R China
第一作者单位	中科院安徽光学精密机械研究所
推荐引用方式 GB/T 7714	Zhang Xiao-Ling,Yin Gao-Fang,Zhao Nan-Jing,et al. Research on Fluorescence Spectral Characteristics of Phycocyanin under Different Habitat Conditions[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS,2017,37(4):1145-1151.
APA	Zhang Xiao-Ling.,Yin Gao-Fang.,Zhao Nan-Jing.,Qin Zhi-Song.,Xiao Xuel.,...&Liu Wen-Qing.(2017).Research on Fluorescence Spectral Characteristics of Phycocyanin under Different Habitat Conditions.SPECTROSCOPY AND SPECTRAL ANALYSIS,37(4),1145-1151.
MLA	Zhang Xiao-Ling,et al."Research on Fluorescence Spectral Characteristics of Phycocyanin under Different Habitat Conditions".SPECTROSCOPY AND SPECTRAL ANALYSIS 37.4(2017):1145-1151.