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Selection of Suitable Reference Genes for Quantitative Real-time PCR in Sapium sebiferum
Chen, Xue1,2,3; Mao, Yingji1,2,3; Huang, Shengwei1,2; Ni, Jun1,2; Lu, Weili1,2,3,4; Hou, Jinyan1,2; Wang, Muting1,2,3,5; Zhao, Weiwei1,2; Li, Minghao1,2; Wang, Qiaojian6; Wu, Lifang1,2
2017-05-04
发表期刊FRONTIERS IN PLANT SCIENCE
卷号8
摘要Chinese tallow (Sapium sebiferum L.) is a promising landscape and bioenergy plant. Measuring gene expression by quantitative real-time polymerase chain reaction (qRT-PCR) can provide valuable information on gene function. Stably expressed reference genes for normalization are a prerequisite for ensuring the accuracy of the target gene expression level among different samples. However, the reference genes in Chinese tallow have not been systematically validated. In this study, 12 candidate reference genes (185, GAPDH, UBQ, RPS15, SAND, TIP41, 60S, ACT7, PDF2, APT, TBP and TUB) were investigated with qRT-PCR in 18 samples, including those from different tissues, from plants treated with sucrose and cold stresses. The data were calculated with four common algorithms, geNorm, BestKeeper, NormFinder, and the delta cycle threshold (Delta Ct). TIP41 and GAPDH were the most stable for the tissue-specific experiment, GAPDH and 60S for cold treatment, and GAPDH and UBQ for sucrose stresses, while the least stable genes were 60S, TIP41, and 18S respectively. The comprehensive results showed APT, GAPDH, and UBQ to be the top-ranked stable genes across all the samples. The stability of 60S was the lowest during all experiments. These selected reference genes were further validated by comparing the expression profiles of the chalcone synthase gene in Chinese tallow in different samples. The results will help to improve the accuracy of gene expression studies in Chinese tallow.
文章类型Article
关键词Chinese Tallow (Sapium Sebiferum) Qrt-pcr Reference Genes Gene Expression Sucrose And Cold Stress
WOS标题词Science & Technology ; Life Sciences & Biomedicine
资助者National Natural Science Foundation of China(11375232) ; National Natural Science Foundation of China(11375232) ; Special Fund for Forest Scientific Research in the Public Welfare(201404110) ; Special Fund for Forest Scientific Research in the Public Welfare(201404110) ; Science and Technology Service program of Chinese Academy of Sciences(KFI-EW-STS-083) ; Science and Technology Service program of Chinese Academy of Sciences(KFI-EW-STS-083) ; President Foundation of Hefei Institutes of Physical Science of Chinese Academy of Sciences(YZJJ201502) ; President Foundation of Hefei Institutes of Physical Science of Chinese Academy of Sciences(YZJJ201502) ; Key deployment project of Chinese Academy of Sciences(KFZD-SW-1.05) ; Key deployment project of Chinese Academy of Sciences(KFZD-SW-1.05) ; National Natural Science Foundation of China(11375232) ; National Natural Science Foundation of China(11375232) ; Special Fund for Forest Scientific Research in the Public Welfare(201404110) ; Special Fund for Forest Scientific Research in the Public Welfare(201404110) ; Science and Technology Service program of Chinese Academy of Sciences(KFI-EW-STS-083) ; Science and Technology Service program of Chinese Academy of Sciences(KFI-EW-STS-083) ; President Foundation of Hefei Institutes of Physical Science of Chinese Academy of Sciences(YZJJ201502) ; President Foundation of Hefei Institutes of Physical Science of Chinese Academy of Sciences(YZJJ201502) ; Key deployment project of Chinese Academy of Sciences(KFZD-SW-1.05) ; Key deployment project of Chinese Academy of Sciences(KFZD-SW-1.05)
DOI10.3389/fpls.2017.00637
关键词[WOS]RT-PCR ; ANTHOCYANIN BIOSYNTHESIS ; HOUSEKEEPING GENES ; LOW-TEMPERATURE ; ARABIDOPSIS-THALIANA ; EXPRESSION ANALYSIS ; NORMALIZATION ; ACCUMULATION ; RED ; IDENTIFICATION
收录类别SCI
语种英语
资助者National Natural Science Foundation of China(11375232) ; National Natural Science Foundation of China(11375232) ; Special Fund for Forest Scientific Research in the Public Welfare(201404110) ; Special Fund for Forest Scientific Research in the Public Welfare(201404110) ; Science and Technology Service program of Chinese Academy of Sciences(KFI-EW-STS-083) ; Science and Technology Service program of Chinese Academy of Sciences(KFI-EW-STS-083) ; President Foundation of Hefei Institutes of Physical Science of Chinese Academy of Sciences(YZJJ201502) ; President Foundation of Hefei Institutes of Physical Science of Chinese Academy of Sciences(YZJJ201502) ; Key deployment project of Chinese Academy of Sciences(KFZD-SW-1.05) ; Key deployment project of Chinese Academy of Sciences(KFZD-SW-1.05) ; National Natural Science Foundation of China(11375232) ; National Natural Science Foundation of China(11375232) ; Special Fund for Forest Scientific Research in the Public Welfare(201404110) ; Special Fund for Forest Scientific Research in the Public Welfare(201404110) ; Science and Technology Service program of Chinese Academy of Sciences(KFI-EW-STS-083) ; Science and Technology Service program of Chinese Academy of Sciences(KFI-EW-STS-083) ; President Foundation of Hefei Institutes of Physical Science of Chinese Academy of Sciences(YZJJ201502) ; President Foundation of Hefei Institutes of Physical Science of Chinese Academy of Sciences(YZJJ201502) ; Key deployment project of Chinese Academy of Sciences(KFZD-SW-1.05) ; Key deployment project of Chinese Academy of Sciences(KFZD-SW-1.05)
WOS研究方向Plant Sciences
WOS类目Plant Sciences
WOS记录号WOS:000400611400001
引用统计
文献类型期刊论文
条目标识符http://ir.hfcas.ac.cn:8080/handle/334002/33517
专题技术生物与农业工程研究所
作者单位1.Chinese Acad Sci, Hefei Inst Phys Sci, Key Lab Ion Beam Bioengn, Hefei, Peoples R China
2.Chinese Acad Sci, Hefei Inst Phys Sci, Bioenergy Forest Res Ctr, State Forestry Adm, Hefei, Peoples R China
3.Univ Sci & Technol China, Sci Isl Branch, Grad Sch, Inst Tech Biol & Agr Engn, Hefei, Peoples R China
4.Anhui Med Univ, Sch Pharm, Hefei, Peoples R China
5.Anhui Acad Agr Sci, Sericultural Res Inst, Hefei, Peoples R China
6.Anhui Agr Univ, Sch Forestry & Landscape Architecture, Hefei, Peoples R China
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Chen, Xue,Mao, Yingji,Huang, Shengwei,et al. Selection of Suitable Reference Genes for Quantitative Real-time PCR in Sapium sebiferum[J]. FRONTIERS IN PLANT SCIENCE,2017,8.
APA Chen, Xue.,Mao, Yingji.,Huang, Shengwei.,Ni, Jun.,Lu, Weili.,...&Wu, Lifang.(2017).Selection of Suitable Reference Genes for Quantitative Real-time PCR in Sapium sebiferum.FRONTIERS IN PLANT SCIENCE,8.
MLA Chen, Xue,et al."Selection of Suitable Reference Genes for Quantitative Real-time PCR in Sapium sebiferum".FRONTIERS IN PLANT SCIENCE 8(2017).
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