SNX16 Regulates the Recycling of E-Cadherin through a Unique Mechanism of Coordinated Membrane and Cargo Binding

doi:10.1016/j.str.2017.06.015

HFCAS OpenIR > 中科院强磁场科学中心

	SNX16 Regulates the Recycling of E-Cadherin through a Unique Mechanism of Coordinated Membrane and Cargo Binding
	Xu, Jinxin 1; Zhang, Leilei 2; Ye, Yinghua 2; Shan, Yongli 2; Wan, Chanjuan 3; Wang, Junfeng 3; Pei, Duanqing 2; Shu, Xiaodong 2; Liu, Jinsong 1,4
	2017-08-01
发表期刊	STRUCTURE
摘要	E-Cadherin is a major component of adherens junctions on cell surfaces. SNX16 is a unique member of sorting nexins that contains a coiled-coil (CC) domain downstream of the PX domain. We report here that SNX16 regulates the recycling trafficking of E-cadherin. We solved the crystal structure of PX-CC unit of SNX16 and revealed a unique shear shaped homodimer. We identified a novel PI3P binding pocket in SNX16 that consists of both the PX and the CC domains. Surprisingly, we showed that the PPII/alpha 2 loop, which is generally regarded as a membrane insertion loop in PX family proteins, is involved in the E-cadherin binding with SNX16. We then proposed a multivalent membrane binding model for SNX16. Our study postulates a new mechanism for coordinated membrane binding and cargo binding for SNX family proteins in general, and provide novel insights into recycling trafficking of E-cadherin.
文章类型	Article
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1016/j.str.2017.06.015
关键词[WOS]	PHOX-HOMOLOGY DOMAIN ; CELL-CELL ADHESION ; PX DOMAIN ; PHOSPHOINOSITIDE-BINDING ; MOLECULAR REPLACEMENT ; TRAFFICKING ; ENDOSOME ; PROTEINS ; RECEPTOR ; METASTASIS
收录类别	SCI
语种	英语
项目资助者	National Basic Research Program of China (973 Program)(2012CB917200) ; National Basic Research Program of China (973 Program)(2012CB917200) ; National Basic Research Program of China (973 Program)(2012CB917200) ; National Basic Research Program of China (973 Program)(2012CB917200) ; National Basic Research Program of China (973 Program)(2012CB917200) ; National Basic Research Program of China (973 Program)(2012CB917200) ; National Basic Research Program of China (973 Program)(2012CB917200) ; National Basic Research Program of China (973 Program)(2012CB917200) ; National Natural Science Foundation of China(31421004 ; National Natural Science Foundation of China(31421004 ; National Natural Science Foundation of China(31421004 ; National Natural Science Foundation of China(31421004 ; National Natural Science Foundation of China(31421004 ; National Natural Science Foundation of China(31421004 ; National Natural Science Foundation of China(31421004 ; National Natural Science Foundation of China(31421004 ; Science and Technology Planning Project of Guangdong Province(2014B030301058) ; Science and Technology Planning Project of Guangdong Province(2014B030301058) ; Science and Technology Planning Project of Guangdong Province(2014B030301058) ; Science and Technology Planning Project of Guangdong Province(2014B030301058) ; Science and Technology Planning Project of Guangdong Province(2014B030301058) ; Science and Technology Planning Project of Guangdong Province(2014B030301058) ; Science and Technology Planning Project of Guangdong Province(2014B030301058) ; Science and Technology Planning Project of Guangdong Province(2014B030301058) ; Natural Science Foundation of Guangdong Province, China(2014A030310356) ; Natural Science Foundation of Guangdong Province, China(2014A030310356) ; Natural Science Foundation of Guangdong Province, China(2014A030310356) ; Natural Science Foundation of Guangdong Province, China(2014A030310356) ; Natural Science Foundation of Guangdong Province, China(2014A030310356) ; Natural Science Foundation of Guangdong Province, China(2014A030310356) ; Natural Science Foundation of Guangdong Province, China(2014A030310356) ; Natural Science Foundation of Guangdong Province, China(2014A030310356) ; Guangzhou Healthcare Collaborative Innovation Programs(201508020255) ; Guangzhou Healthcare Collaborative Innovation Programs(201508020255) ; Guangzhou Healthcare Collaborative Innovation Programs(201508020255) ; Guangzhou Healthcare Collaborative Innovation Programs(201508020255) ; Guangzhou Healthcare Collaborative Innovation Programs(201508020255) ; Guangzhou Healthcare Collaborative Innovation Programs(201508020255) ; Guangzhou Healthcare Collaborative Innovation Programs(201508020255) ; Guangzhou Healthcare Collaborative Innovation Programs(201508020255) ; Guangzhou Branch of the Supercomputing Center of CAS ; Guangzhou Branch of the Supercomputing Center of CAS ; Guangzhou Branch of the Supercomputing Center of CAS ; Guangzhou Branch of the Supercomputing Center of CAS ; Guangzhou Branch of the Supercomputing Center of CAS ; Guangzhou Branch of the Supercomputing Center of CAS ; Guangzhou Branch of the Supercomputing Center of CAS ; Guangzhou Branch of the Supercomputing Center of CAS ; 31271502 ; 31271502 ; 31271502 ; 31271502 ; 31271502 ; 31271502 ; 31271502 ; 31271502 ; 31570759 ; 31570759 ; 31570759 ; 31570759 ; 31570759 ; 31570759 ; 31570759 ; 31570759 ; 30870496) ; 30870496) ; 30870496) ; 30870496) ; 30870496) ; 30870496) ; 30870496) ; 30870496)
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics ; Cell Biology
WOS类目	Biochemistry & Molecular Biology ; Biophysics ; Cell Biology
WOS记录号	WOS:000406744600009
引用统计	被引频次：15[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.hfcas.ac.cn:8080/handle/334002/33562
专题	中科院强磁场科学中心
作者单位	1.Chinese Acad Sci, Guangzhou Inst Biomed & Hlth, State Key Lab Resp Dis, Guangzhou 510530, Guangdong, Peoples R China 2.Chinese Acad Sci, Guangzhou Inst Biomed & Hlth, Guangzhou Med Univ Joint Sch Biol Sci,South China, CAS Key Lab Regenerat Biol,Guangdong Key Lab Stem, Guangzhou 510530, Guangdong, Peoples R China 3.Chinese Acad Sci, High Magnet Field Lab, Hefei Inst Phys Sci, Hefei 230031, Peoples R China 4.Chinese Acad Sci, Guangzhou Inst Biomed & Hlth, Guangdong Prov Key Lab Biocomp, Guangzhou 510530, Guangdong, Peoples R China
推荐引用方式 GB/T 7714	Xu, Jinxin,Zhang, Leilei,Ye, Yinghua,et al. SNX16 Regulates the Recycling of E-Cadherin through a Unique Mechanism of Coordinated Membrane and Cargo Binding[J]. STRUCTURE,2017,25(8):1251-+.
APA	Xu, Jinxin.,Zhang, Leilei.,Ye, Yinghua.,Shan, Yongli.,Wan, Chanjuan.,...&Liu, Jinsong.(2017).SNX16 Regulates the Recycling of E-Cadherin through a Unique Mechanism of Coordinated Membrane and Cargo Binding.STRUCTURE,25(8),1251-+.
MLA	Xu, Jinxin,et al."SNX16 Regulates the Recycling of E-Cadherin through a Unique Mechanism of Coordinated Membrane and Cargo Binding".STRUCTURE 25.8(2017):1251-+.