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Non-canonical regulation of SPL transcription factors by a human OTUB1-like deubiquitinase defines a new plant type rice associated with higher grain yield
Wang, Shuansuo1; Wu, Kun2,3; Qian, Qian4; Liu, Qian1; Li, Qi1; Pan, Yajun1; Ye, Yafeng1; Liu, Xueying1; Wang, Jing1; Zhang, Jianqing1; Li, Shan1; Wu, Yuejin2; Fu, Xiangdong1,5
2017-09-01
Source PublicationCELL RESEARCH
Volume27Issue:9Pages:1142-1156
AbstractAchieving increased grain productivity has long been the overriding focus of cereal breeding programs. The ideotype approach has been used to improve rice yield potential at the International Rice Research Institute and in China. However, the genetic basis of yield-related traits in rice remains unclear. Here, we show that a major quantitative trait locus, qNPT1, acts through the determination of a 'new plant type' (NPT) architecture characterized by fewer tillers, sturdier culms and larger panicles, and it encodes a deubiquitinating enzyme with homology to human OTUB1. Downregulation of OsOTUB1 enhances meristematic activity, resulting in reduced tiller number, increased grain number, enhanced grain weight and a consequent increase in grain yield in rice. Unlike human OTUB1, OsOTUB1 can cleave both K48- and K63-linked polyubiquitin. OsOTUB1 interacts with the E2 ubiquitin-conjugating protein OsUBC13 and the squamosa promoter-binding protein-like transcription factor OsSPL14. OsOTUB1 and OsSPL14 share common target genes, and their physical interaction limits K63-linked ubiquitination (K63Ub) of OsSPL14, which in turn promotes K48Ub-dependent proteasomal degradation of OsSPL14. Conversely, loss-of-function of OsOTUB1 is correlated with the accumulation of high levels of OsSPL14, resulting in the NPT architecture. We also demonstrated that pyramiding of high-yielding npt1 and dep1-1 alleles provides a new strategy for increasing rice yield potential above what is currently achievable.
SubtypeArticle
KeywordNew Plant Type Rice Osotub1 Deubiquitination Osspl14 Grain Yield
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Key Research and Development Program of China(2016YFD0100401) ; National Key Research and Development Program of China(2016YFD0100401) ; Chinese Academy of Sciences(XDA08010101) ; Chinese Academy of Sciences(XDA08010101) ; National Natural Science Foundation of China(91635302) ; National Natural Science Foundation of China(91635302) ; National Key Research and Development Program of China(2016YFD0100401) ; National Key Research and Development Program of China(2016YFD0100401) ; Chinese Academy of Sciences(XDA08010101) ; Chinese Academy of Sciences(XDA08010101) ; National Natural Science Foundation of China(91635302) ; National Natural Science Foundation of China(91635302)
DOI10.1038/cr.2017.98
WOS KeywordNATURAL VARIATION ; GREEN-REVOLUTION ; INCREASES YIELD ; USE EFFICIENCY ; ARABIDOPSIS ; PROTEIN ; UBIQUITINATION ; ARCHITECTURE ; GENE ; INHIBITION
Indexed BySCI
Language英语
Funding OrganizationNational Key Research and Development Program of China(2016YFD0100401) ; National Key Research and Development Program of China(2016YFD0100401) ; Chinese Academy of Sciences(XDA08010101) ; Chinese Academy of Sciences(XDA08010101) ; National Natural Science Foundation of China(91635302) ; National Natural Science Foundation of China(91635302) ; National Key Research and Development Program of China(2016YFD0100401) ; National Key Research and Development Program of China(2016YFD0100401) ; Chinese Academy of Sciences(XDA08010101) ; Chinese Academy of Sciences(XDA08010101) ; National Natural Science Foundation of China(91635302) ; National Natural Science Foundation of China(91635302)
WOS Research AreaCell Biology
WOS SubjectCell Biology
WOS IDWOS:000408916300009
Citation statistics
Cited Times:15[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.hfcas.ac.cn:8080/handle/334002/33620
Collection中科院强磁场科学中心
Affiliation1.Chinese Acad Sci, Natl Ctr Plant Gene Res, Inst Genet & Dev Biol, State Key Lab Plant Cell & Chromosome Engn, Beijing 100101, Peoples R China
2.Chinese Acad Sci, Hefei Inst Phys Sci, Key Lab High Magnet Field & Ion Beam Phys Biol, Hefei 230031, Anhui, Peoples R China
3.Univ Sci & Technol China, Sch Life Sci, Hefei 230000, Anhui, Peoples R China
4.China Natl Rice Res Inst, Key Lab Rice Biol, Hangzhou 310006, Zhejiang, Peoples R China
5.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Wang, Shuansuo,Wu, Kun,Qian, Qian,et al. Non-canonical regulation of SPL transcription factors by a human OTUB1-like deubiquitinase defines a new plant type rice associated with higher grain yield[J]. CELL RESEARCH,2017,27(9):1142-1156.
APA Wang, Shuansuo.,Wu, Kun.,Qian, Qian.,Liu, Qian.,Li, Qi.,...&Fu, Xiangdong.(2017).Non-canonical regulation of SPL transcription factors by a human OTUB1-like deubiquitinase defines a new plant type rice associated with higher grain yield.CELL RESEARCH,27(9),1142-1156.
MLA Wang, Shuansuo,et al."Non-canonical regulation of SPL transcription factors by a human OTUB1-like deubiquitinase defines a new plant type rice associated with higher grain yield".CELL RESEARCH 27.9(2017):1142-1156.
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