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A diubiquitin-based photoaffinity probe for profiling K27-linkage targeting deubiquitinases
Tan, Xiao-Dan1,2; Pan, Man1; Gao, Shuai1; Zheng, Yong2,3,4; Shi, Jing3,4; Li, Yi-Ming2
2017-09-18
Source PublicationCHEMICAL COMMUNICATIONS
Volume53Issue:73Pages:10208-10211
AbstractK27-linkage poly-ubiquitination plays important roles in DNA damage repair and autoimmunity. Identification of K27-linkage targeting deubiquitinases (DUBs) is essential for understanding their regulatory mechanisms. Here we report an aryl-azide-based photo-affinity diubiquitin (diUb) probe for profiling K27-linkage targeting DUBs. This probe shows high selectivity and sensitivity towards K27-linkage DUBs in vitro. It can also be used in cell lysate for identifying and probing K27-linkage targeting DUBs in real proteomes. Our work suggests that the photoaffinity-based strategy may provide a new useful approach for the construction of other isopeptide linkage targeting DUB probes.
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
Funding OrganizationNational Natural Science Foundation of China(21372058 ; National Natural Science Foundation of China(21372058 ; 21572043 ; 21572043 ; 21572214) ; 21572214) ; National Natural Science Foundation of China(21372058 ; National Natural Science Foundation of China(21372058 ; 21572043 ; 21572043 ; 21572214) ; 21572214)
DOI10.1039/c7cc05504h
WOS KeywordPROTEIN CHEMICAL-SYNTHESIS ; PEPTIDE HYDRAZIDES ; UBIQUITIN CHAINS ; POSTTRANSLATIONAL MODIFICATIONS ; POLYUBIQUITIN CHAINS ; DNA-DAMAGE ; SPECIFICITY ; LIGATION ; ENZYMES ; LYSINE
Indexed BySCI
Language英语
Funding OrganizationNational Natural Science Foundation of China(21372058 ; National Natural Science Foundation of China(21372058 ; 21572043 ; 21572043 ; 21572214) ; 21572214) ; National Natural Science Foundation of China(21372058 ; National Natural Science Foundation of China(21372058 ; 21572043 ; 21572043 ; 21572214) ; 21572214)
WOS Research AreaChemistry
WOS SubjectChemistry, Multidisciplinary
WOS IDWOS:000410360700027
Citation statistics
Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.hfcas.ac.cn:8080/handle/334002/33657
Collection中科院强磁场科学中心
Affiliation1.Tsinghua Univ, Dept Chem, Key Lab Bioorgan Phosphorus Chem & Chem Biol, Tsinghua Peking Ctr Life Sci,Minist Educ, Beijing 100084, Peoples R China
2.Hefei Univ Technol, Sch Biol & Med Engn, Hefei 230009, Anhui, Peoples R China
3.Univ Sci & Technol China, Dept Chem, Hefei 230026, Anhui, Peoples R China
4.Chinese Acad Sci, High Field Magnet Lab, Hefei 230026, Anhui, Peoples R China
Recommended Citation
GB/T 7714
Tan, Xiao-Dan,Pan, Man,Gao, Shuai,et al. A diubiquitin-based photoaffinity probe for profiling K27-linkage targeting deubiquitinases[J]. CHEMICAL COMMUNICATIONS,2017,53(73):10208-10211.
APA Tan, Xiao-Dan,Pan, Man,Gao, Shuai,Zheng, Yong,Shi, Jing,&Li, Yi-Ming.(2017).A diubiquitin-based photoaffinity probe for profiling K27-linkage targeting deubiquitinases.CHEMICAL COMMUNICATIONS,53(73),10208-10211.
MLA Tan, Xiao-Dan,et al."A diubiquitin-based photoaffinity probe for profiling K27-linkage targeting deubiquitinases".CHEMICAL COMMUNICATIONS 53.73(2017):10208-10211.
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