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Improved chemical synthesis of o-nirtrobenzyl-tyrosine for concise site-specific N-15-tyrosine NMR analysis demonstrated by plant ABA receptor PYL10
Guo, Xiaoqi1,2,3; Zhang, Bo1,2; He, Yao1,2; Liu, Yangzhong3; Tian, Changlin1,2
2017-09-27
Source PublicationTETRAHEDRON LETTERS
Volume58Issue:39Pages:3764-3767
AbstractThe yield of unnatural amino acid o-nitrobenzyl-tyrosine (oNBTyr), which was able to recover to natural tyrosine after UV-photocleavage was greatly improved from 20% to 81% by using 2-nitrobenzyl bromide as the nucleophilic reagent. Through genetically introducing N-15-oNBY and consequent photo-cleavage, the site-specific N-15-Tyr NMR analysis of plant ABA (abscisic acid) receptor PYL10 was implemented without any residue variation. This isotope labelling of tyrosine onto protein backbone provides a convenient strategy for NMR analysis. (C) 2017 Published by Elsevier Ltd.
SubtypeArticle
KeywordUnnatural Amino Acid Tyrosine Protein Nmr Analysis Abscisic Acid Receptor Pyl-10
WOS HeadingsScience & Technology ; Physical Sciences
Funding OrganizationChinese Key Research Plan Protein Science(2015CB910100 ; Chinese Key Research Plan Protein Science(2015CB910100 ; Chinese Natural Science Foundation(U1332138 ; Chinese Natural Science Foundation(U1332138 ; 2016YFA0400900) ; 2016YFA0400900) ; U1432136) ; U1432136) ; Chinese Key Research Plan Protein Science(2015CB910100 ; Chinese Key Research Plan Protein Science(2015CB910100 ; Chinese Natural Science Foundation(U1332138 ; Chinese Natural Science Foundation(U1332138 ; 2016YFA0400900) ; 2016YFA0400900) ; U1432136) ; U1432136)
DOI10.1016/j.tetlet.2017.08.034
WOS KeywordABSCISIC-ACID ; PROTEIN ; DYNAMICS ; SPECTROSCOPY ; ARABIDOPSIS ; BACKBONE ; COMPLEX ; PROBE
Indexed BySCI
Language英语
Funding OrganizationChinese Key Research Plan Protein Science(2015CB910100 ; Chinese Key Research Plan Protein Science(2015CB910100 ; Chinese Natural Science Foundation(U1332138 ; Chinese Natural Science Foundation(U1332138 ; 2016YFA0400900) ; 2016YFA0400900) ; U1432136) ; U1432136) ; Chinese Key Research Plan Protein Science(2015CB910100 ; Chinese Key Research Plan Protein Science(2015CB910100 ; Chinese Natural Science Foundation(U1332138 ; Chinese Natural Science Foundation(U1332138 ; 2016YFA0400900) ; 2016YFA0400900) ; U1432136) ; U1432136)
WOS Research AreaChemistry
WOS SubjectChemistry, Organic
WOS IDWOS:000412041400003
Citation statistics
Document Type期刊论文
Identifierhttp://ir.hfcas.ac.cn:8080/handle/334002/33764
Collection中科院强磁场科学中心
Affiliation1.Chinese Acad Sci, High Magnet Field Lab, Hefei, Anhui, Peoples R China
2.Univ Sci & Technol China, Hefei Nat Lab Phys Sci Microscale, Sch Life Sci, Hefei 230026, Anhui, Peoples R China
3.Univ Sci & Technol China, Sch Chem & Mat Sci, Hefei 230026, Anhui, Peoples R China
Recommended Citation
GB/T 7714
Guo, Xiaoqi,Zhang, Bo,He, Yao,et al. Improved chemical synthesis of o-nirtrobenzyl-tyrosine for concise site-specific N-15-tyrosine NMR analysis demonstrated by plant ABA receptor PYL10[J]. TETRAHEDRON LETTERS,2017,58(39):3764-3767.
APA Guo, Xiaoqi,Zhang, Bo,He, Yao,Liu, Yangzhong,&Tian, Changlin.(2017).Improved chemical synthesis of o-nirtrobenzyl-tyrosine for concise site-specific N-15-tyrosine NMR analysis demonstrated by plant ABA receptor PYL10.TETRAHEDRON LETTERS,58(39),3764-3767.
MLA Guo, Xiaoqi,et al."Improved chemical synthesis of o-nirtrobenzyl-tyrosine for concise site-specific N-15-tyrosine NMR analysis demonstrated by plant ABA receptor PYL10".TETRAHEDRON LETTERS 58.39(2017):3764-3767.
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