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Enhanced DNA double-strand break repair of microbeam targeted A549 lung carcinoma cells by adjacent WI38 normal lung fibroblast cells via bidirectional signaling
Kobayashi, Alisa1,2,3; Farizal Tengku Ahmad, Tengku Ahbrizal1,4; Autsavapromporn, Narongchai1,5; Oikawa, Masakazu1,2; Homma-Takeda, Shino6; Furusawa, Yoshiya1,6; Wang, Jun1,7; Konishi, Teruaki1,6
2017-10-01
Source PublicationMUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS
Volume803Issue:Pages:1-8
AbstractUnderstanding the mechanisms underlying the radiation-induced bystander effect (RIBE) and bi-directional signaling between irradiated carcinoma cells and their surrounding non-irradiated normal cells is relevant to cancer radiotherapy. The present study investigated propagation of RIBE signals between human lung carcinoma A549 cells and normal lung fibroblast WI38 cells in bystander cells, either directly or indirectly contacting irradiated A549 cells. We prepared A549-GFP/WI38 co-cultures and A549-GFP/A549 co-cultures, in which A549-GFP cells stably expressing H2BGFP were co-cultured with either A549 cells or WI38 cells, respectively. Using the SPICE-NIRS microbeam, only the A549-GFP cells were irradiated with 500 protons per cell. The level of gamma-H2AX, a marker for DNA double-strand breaks (DSB), was subsequently measured for up to 24 h postirradiation in three categories of cells: (1) "targeted"/irradiated A549-GFP cells; (2) "neighboring"/non-irradiated cells directly contacting the "targeted" cells; and (3) "distant"/non-irradiated cells, which were not in direct contact with the "targeted" cells. We found that DSB repair in targeted A549-GFP cells was enhanced by co-cultured WI38 cells. The bystander response in A549-GFP/A549 cell co-cultures, as marked by gamma-H2AX levels at 8 h post-irradiation, showed a decrease to non-irradiated control level when approaching 24 h, while the neighboring/distant bystander WI38 cells in A549-GFP/WI38 co-cultures was maintained at a similar level until 24 h post-irradiation. Surprisingly, distant A549-GFP cells in A549-GFP/WI38 co-cultures showed time dependency similar to bystander WI38 cells, but not to distant cells in A549-GFP/A549 co-cultures. These observations indicate that gamma-H2AX was induced in WI38 cells as a result of RIBE. WI38 cells were not only involved in rescue of targeted A549, but also in the modification of RIBE against distant A549-GFP cells. The present results demonstrate that radiation-induced bi-directional signaling had extended a profound influence on cellular sensitivity to radiation as well as the sensitivity to RIBE.
SubtypeArticle
KeywordProton Microbeam Lung Cells Gamma-h2ax Radiation Induced Bystander Effect Rescue Effect
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationJSPS KAKENHI(25861137) ; NIRS-Sharing of advanced research infrastructure(S13-TK01 ; S16-AK01) ; JSPS KAKENHI(25861137) ; NIRS-Sharing of advanced research infrastructure(S13-TK01 ; S16-AK01)
DOI10.1016/j.mrfmmm.2017.06.006
WOS KeywordCARBON ION RADIOTHERAPY ; ALPHA-PARTICLES ; CANCER-CELLS ; STEM-LIKE ; X-RAYS ; BYSTANDER ; RADIATION ; IRRADIATION ; COMMUNICATION ; MODULATION
Indexed BySCI
Language英语
Funding OrganizationJSPS KAKENHI(25861137) ; NIRS-Sharing of advanced research infrastructure(S13-TK01 ; S16-AK01) ; JSPS KAKENHI(25861137) ; NIRS-Sharing of advanced research infrastructure(S13-TK01 ; S16-AK01)
WOS Research AreaBiotechnology & Applied Microbiology ; Genetics & Heredity ; Toxicology
WOS SubjectBiotechnology & Applied Microbiology ; Genetics & Heredity ; Toxicology
WOS IDWOS:000413598800001
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.hfcas.ac.cn:8080/handle/334002/33809
Collection技术生物与农业工程研究所
Affiliation1.Natl Inst Quantum & Radiol Sci & Technol, Natl Inst Radiol Sci, NIRS Int Open Lab, SPICE BIO Res Core,Inage Ku, Anagawa 4-9-1, Chiba 2638555, Japan
2.Natl Inst Quantum & Radiol Sci & Technol, Natl Inst Radiol Sci, Dept Accelerator & Med Phys, Inage Ku, Anagawa 4-9-1, Chiba 2638555, Japan
3.Univ Tsukuba, Grad Sch Comprehens Human Sci, 1-1-1 Tennodai, Tsukuba, Ibaraki 3058575, Japan
4.Agensi Nuklear Malaysia, Div Agrotechnol & Biosci, Bangi 43000, Kajang, Malaysia
5.Chiang Mai Univ, Div Therapeut Radiol & Oncol, Dept Radiol, Fac Med, Chiang Mai, Thailand
6.Natl Inst Quantum & Radiol Sci & Technol, Natl Inst Radiol Sci, Dept Basic Med Sci Radiat Damages, Inage Ku, Anagawa 4-9-1, Chiba 2638555, Japan
7.Chinese Acad Sci & Anhui Prov, Hefei Inst Phys Sci, Key Lab Ion Beam Bioengn, 350 Shushanhu Rd, Hefei 230031, Anhui, Peoples R China
Recommended Citation
GB/T 7714
Kobayashi, Alisa,Farizal Tengku Ahmad, Tengku Ahbrizal,Autsavapromporn, Narongchai,et al. Enhanced DNA double-strand break repair of microbeam targeted A549 lung carcinoma cells by adjacent WI38 normal lung fibroblast cells via bidirectional signaling[J]. MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS,2017,803(无):1-8.
APA Kobayashi, Alisa.,Farizal Tengku Ahmad, Tengku Ahbrizal.,Autsavapromporn, Narongchai.,Oikawa, Masakazu.,Homma-Takeda, Shino.,...&Konishi, Teruaki.(2017).Enhanced DNA double-strand break repair of microbeam targeted A549 lung carcinoma cells by adjacent WI38 normal lung fibroblast cells via bidirectional signaling.MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS,803(无),1-8.
MLA Kobayashi, Alisa,et al."Enhanced DNA double-strand break repair of microbeam targeted A549 lung carcinoma cells by adjacent WI38 normal lung fibroblast cells via bidirectional signaling".MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS 803.无(2017):1-8.
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