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Microfluidic dielectrophoresis device for trapping, counting and detecting Shewanella oneidensis at the cell level
Chen, Xiangyu1,2; Liang, Zhiting1; Li, Daobo3; Xiong, Ying1; Xiong, Penghui1; Guan, Yong1; Hou, Shuangyue1; Hu, Yue1; Chen, Shan1; Liu, Gang1; Tian, Yangchao1
2018-01-15
发表期刊BIOSENSORS & BIOELECTRONICS
卷号99期号:页码:416-423
摘要Shewanella oneidensis, a model organism for electrochemical activity bacteria, has been widely studied at the biofilm level. However, to obtain more information regarding this species, it is essential to develop an approach to trap and detect S. oneidensis at the cell level. In this study, we report a rapid and label-free microfluidic platform for trapping, counting and detecting S. oneidensis cells. A microfluidic chip was integrated with a modified dielectrophoresis (DEP) trapping technique and hole arrays of different hole sizes. By numerical simulation and an elaborate electric field distribution design, S. oneidensis cells were successfully trapped and positioned in the hole arrays. Real time fluorescence imaging was also used to observe the trapping process. With the aid of a homemade image program, the trapped bacteria were accurately counted, and the results demonstrated that the amount of bacteria correlated with the hole sizes. As one of the significant applications of the device, Raman identification and detection of countable S. oneidensis cells was accomplished in two kinds of holes. The microfluidic platform provides a quantitative sample preparation and analysis method at the cell level that could be widely applied in the environmental and energy fields.
文章类型Article
关键词Microfluidic Chip Dielectrophoresis Shewanella Oneidensis Fluorescence Cell Level
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
资助者National Key Basic Research Program of China(2016YFA0400902) ; National Science Foundation of China(11275204 ; Dean Foundation of Hefei Institute of Physical Science, CAS(YZJJ201514) ; 11475175 ; 11405175 ; 21507123) ; National Key Basic Research Program of China(2016YFA0400902) ; National Science Foundation of China(11275204 ; Dean Foundation of Hefei Institute of Physical Science, CAS(YZJJ201514) ; 11475175 ; 11405175 ; 21507123)
DOI10.1016/j.bios.2017.08.017
关键词[WOS]LABEL-FREE DETECTION ; ESCHERICHIA-COLI ; TECHNOLOGY ; MR-1 ; BIODECOLORIZATION ; SEPARATION ; BACTERIA ; WATER ; NOV
收录类别SCI
语种英语
资助者National Key Basic Research Program of China(2016YFA0400902) ; National Science Foundation of China(11275204 ; Dean Foundation of Hefei Institute of Physical Science, CAS(YZJJ201514) ; 11475175 ; 11405175 ; 21507123) ; National Key Basic Research Program of China(2016YFA0400902) ; National Science Foundation of China(11275204 ; Dean Foundation of Hefei Institute of Physical Science, CAS(YZJJ201514) ; 11475175 ; 11405175 ; 21507123)
WOS研究方向Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
WOS类目Biophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology
WOS记录号WOS:000413284200056
引用统计
被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.hfcas.ac.cn:8080/handle/334002/33813
专题中科院合肥智能机械研究所
作者单位1.Univ Sci & Technol China, Natl Synchrotron Radiat Lab, Hefei 230029, Anhui, Peoples R China
2.Chinese Acad Sci, Inst Intelligent Machines, Hefei 230031, Anhui, Peoples R China
3.Univ Sci & Technol China, Dept Chem, Hefei 230026, Anhui, Peoples R China
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GB/T 7714
Chen, Xiangyu,Liang, Zhiting,Li, Daobo,et al. Microfluidic dielectrophoresis device for trapping, counting and detecting Shewanella oneidensis at the cell level[J]. BIOSENSORS & BIOELECTRONICS,2018,99(无):416-423.
APA Chen, Xiangyu.,Liang, Zhiting.,Li, Daobo.,Xiong, Ying.,Xiong, Penghui.,...&Tian, Yangchao.(2018).Microfluidic dielectrophoresis device for trapping, counting and detecting Shewanella oneidensis at the cell level.BIOSENSORS & BIOELECTRONICS,99(无),416-423.
MLA Chen, Xiangyu,et al."Microfluidic dielectrophoresis device for trapping, counting and detecting Shewanella oneidensis at the cell level".BIOSENSORS & BIOELECTRONICS 99.无(2018):416-423.
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