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Colorimetric and SERS dual-readout for assaying alkaline phosphatase activity by ascorbic acid induced aggregation of Ag coated Au nanoparticles
Zhang, Jian1; He, Lifang1; Zhang, Xin1; Wang, Jianping2; Yang, Liang2; Liu, Bianhua2; Jiang, Changlong2; Zhang, Zhongping3
2017-12-01
发表期刊SENSORS AND ACTUATORS B-CHEMICAL
卷号253期号:页码:839-845
摘要As an essential enzyme, alkaline phosphatase (ALP) has attracted considerable attention for its regulating effect to the dephosphorylation process in living organisms. Here, we present a colorimetric and surface enhanced Raman scattering (SERS) dual-readout approach for assaying ALP activity. The major advantage of dual-readout assay is the perfect combination of the merit of colorimetric and SERS, which not only allow rapid preliminary discrimination of ALP activity by the naked eye but also greatly improved the detection sensitivity by SERS. Specifically, 4-mercaptophenylboronic acid modified Ag coated gold nanoparticles (4-MPBA-Au@Ag NPs) are employed as the colorimetric and SERS bifunctional reporting nanoprobes. Upon the presence of ALP, the phosphate group in the ascorbic acid 2-phosphate (AAP) is cleaved to produce ascorbic acid (AA), which acted as boronic acid moieties receptors to control the aggregation of 4-MPBA-Au@Ag NPs. The color of 4-MPBA-Au@Ag NPs solutions had changed from bright orange to light brown to dark gray, simultaneously accompanied by a substantial enhancement of SERS-readouts for the strong Raman hot-spots between the aggregation of 4-MPBA-Au@Ag NPs. A distinguishable change in the color was observed at an ALP activity of 5.0 U/L, meanwhile, SERS-readout sensing method showed a good linear relationship from 0.50 to 10.0 U/L (R = 0.997) with an exciting detection limit of 0.10 U/L (signal-to-noise ratio of 3). In addition, the dual-readout approach developed here was applied for ALP inhibitor evaluation. With the simple, rapid/direct readout yet outstanding sensitivity, we anticipate that this method would greatly promote practical application in ALP-related early-stage diseases diagnosis. (C) 2017 Elsevier B.V. All rights reserved.
文章类型Article
关键词Alp Activity Dephosphorylation Dual-readout Colorimetric Surface Enhanced Raman Scattering Ag Coated Gold Nanoparticles
WOS标题词Science & Technology ; Physical Sciences ; Technology
资助者Science and National Natural Science Foundation of China(21507134 ; National Basic Research Program of China(2015CB932002) ; China-Singapore Joint Project(2015DFG92510) ; Natural Science Foundation of Anhui Province(1408085QB29 ; Scientific Research Foundation of Anhui Agricultural University(yj2016-06) ; Youth Research Foundation of Anhui Agricultural University(2015zr007) ; 21371174 ; 1508085QB29) ; 21605144 ; 61603001) ; Science and National Natural Science Foundation of China(21507134 ; National Basic Research Program of China(2015CB932002) ; China-Singapore Joint Project(2015DFG92510) ; Natural Science Foundation of Anhui Province(1408085QB29 ; Scientific Research Foundation of Anhui Agricultural University(yj2016-06) ; Youth Research Foundation of Anhui Agricultural University(2015zr007) ; 21371174 ; 1508085QB29) ; 21605144 ; 61603001)
DOI10.1016/j.snb.2017.06.186
关键词[WOS]GOLD NANOPARTICLES ; LABEL-FREE ; ULTRASENSITIVE DETECTION ; FLUORESCENT ; STRATEGY ; SENSOR ; IMMUNOASSAY ; ELECTRODE ; RESIDUES
收录类别SCI
语种英语
资助者Science and National Natural Science Foundation of China(21507134 ; National Basic Research Program of China(2015CB932002) ; China-Singapore Joint Project(2015DFG92510) ; Natural Science Foundation of Anhui Province(1408085QB29 ; Scientific Research Foundation of Anhui Agricultural University(yj2016-06) ; Youth Research Foundation of Anhui Agricultural University(2015zr007) ; 21371174 ; 1508085QB29) ; 21605144 ; 61603001) ; Science and National Natural Science Foundation of China(21507134 ; National Basic Research Program of China(2015CB932002) ; China-Singapore Joint Project(2015DFG92510) ; Natural Science Foundation of Anhui Province(1408085QB29 ; Scientific Research Foundation of Anhui Agricultural University(yj2016-06) ; Youth Research Foundation of Anhui Agricultural University(2015zr007) ; 21371174 ; 1508085QB29) ; 21605144 ; 61603001)
WOS研究方向Chemistry ; Electrochemistry ; Instruments & Instrumentation
WOS类目Chemistry, Analytical ; Electrochemistry ; Instruments & Instrumentation
WOS记录号WOS:000411124800097
引用统计
被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.hfcas.ac.cn:8080/handle/334002/33823
专题中科院合肥智能机械研究所
作者单位1.Anhui Agr Univ, Dept Appl Chem, Hefei 230036, Anhui, Peoples R China
2.Chinese Acad Sci, Inst Intelligent Machines, CAS Ctr Excellence Nanosci, Hefei 230031, Anhui, Peoples R China
3.Anhui Univ, Sch Chem & Chem Engn, Hefei 230601, Anhui, Peoples R China
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Zhang, Jian,He, Lifang,Zhang, Xin,et al. Colorimetric and SERS dual-readout for assaying alkaline phosphatase activity by ascorbic acid induced aggregation of Ag coated Au nanoparticles[J]. SENSORS AND ACTUATORS B-CHEMICAL,2017,253(无):839-845.
APA Zhang, Jian.,He, Lifang.,Zhang, Xin.,Wang, Jianping.,Yang, Liang.,...&Zhang, Zhongping.(2017).Colorimetric and SERS dual-readout for assaying alkaline phosphatase activity by ascorbic acid induced aggregation of Ag coated Au nanoparticles.SENSORS AND ACTUATORS B-CHEMICAL,253(无),839-845.
MLA Zhang, Jian,et al."Colorimetric and SERS dual-readout for assaying alkaline phosphatase activity by ascorbic acid induced aggregation of Ag coated Au nanoparticles".SENSORS AND ACTUATORS B-CHEMICAL 253.无(2017):839-845.
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