HFCAS OpenIR  > 应用技术研究所
M-kit: A cell migration assay based on microfluidic device and smartphone
Yang, Ke1,2; Wu, Jiandong2; Peretz-Soroka, Hagit2; Zhu, Ling1; Li, Zhigang1; Sang, Yaoshuo1; Hipolito, Jolly2; Zhang, Michael3; Santos, Susy4,5; Hillier, Craig3; de Faria, Ricardo Lobato3; Liu, Yong1; Lin, Francis2,6,7,8
2018-01-15
Source PublicationBIOSENSORS & BIOELECTRONICS
Volume99Issue:Pages:259-267
AbstractMobile sensing based on the integration of microfluidic device and smartphone, so-called MS2 technology, has enabled many applications over recent years, and continues to stimulate growing interest in both research communities and industries. In particular, it has been envisioned that MS2 technology can be developed for various cell functional assays to enable basic research and clinical applications. Toward this direction, in this paper, we describe the development of a MS2-based cell functional assay for testing cell migration (the Midt). The system is constructed as an integrated test kit, which includes microfluidic chips, a smartphone-based imaging platform, the phone apps for image capturing and data analysis, and a set of reagent and accessories for performing the cell migration assay. We demonstrated that the Mkit can effectively measure purified neutrophil and cancer cell chemotaxis. Furthermore, neutrophil chemotaxis can be tested from a drop of whole blood using the Midt with red blood cell (RBC) lysis. The effects of chemoattractant dose and gradient profile on neutrophil chemotaxis were also tested using the Midt. In addition to research applications, we demonstrated the effective use of the Mkit for on-site test at the hospital and for testing clinical samples from chronic obstructive pulmonary disease patient. Thus, this developed Mkit provides an easy and integrated experimental platform for cell migration related research and potential medical diagnostic applications.
SubtypeArticle
KeywordMicrofluidic Device Smartphone Cell Functional Assay Cell Migration Chemotaxis
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
Funding OrganizationNatural Sciences and Engineering Research Council of Canada (NSERC)(RGPIN-2014-04789) ; Canadian Institutes of Health Research (CIHR)(CPG-134755) ; CMC Microsystems(3699) ; Winnipeg Rh Institute Foundation ; University of Manitoba ; Mitacs ; NSERC ; Natural Sciences and Engineering Research Council of Canada (NSERC)(RGPIN-2014-04789) ; Canadian Institutes of Health Research (CIHR)(CPG-134755) ; CMC Microsystems(3699) ; Winnipeg Rh Institute Foundation ; University of Manitoba ; Mitacs ; NSERC
DOI10.1016/j.bios.2017.07.064
WOS KeywordPOINT-OF-CARE ; NEUTROPHIL CHEMOTAXIS ; BIOMEDICAL-RESEARCH ; MINI-MICROSCOPE ; MOBILE PHONE ; BLOOD ; PLATFORM ; SYSTEM ; INFLAMMATION ; TUMOR
Indexed BySCI
Language英语
Funding OrganizationNatural Sciences and Engineering Research Council of Canada (NSERC)(RGPIN-2014-04789) ; Canadian Institutes of Health Research (CIHR)(CPG-134755) ; CMC Microsystems(3699) ; Winnipeg Rh Institute Foundation ; University of Manitoba ; Mitacs ; NSERC ; Natural Sciences and Engineering Research Council of Canada (NSERC)(RGPIN-2014-04789) ; Canadian Institutes of Health Research (CIHR)(CPG-134755) ; CMC Microsystems(3699) ; Winnipeg Rh Institute Foundation ; University of Manitoba ; Mitacs ; NSERC
WOS Research AreaBiophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
WOS SubjectBiophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology
WOS IDWOS:000413284200036
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.hfcas.ac.cn:8080/handle/334002/33829
Collection应用技术研究所
Affiliation1.Chinese Acad Sci, Hefei Inst Phys Sci, Inst Appl Technol, Hefei, Anhui, Peoples R China
2.Univ Manitoba, Dept Phys & Astron, Winnipeg, MB, Canada
3.Seven Oaks Gen Hosp, Winnipeg, MB, Canada
4.Victoria Gen Hosp, Winnipeg, MB, Canada
5.River Hts Ft Garry Community Areas, Winnipeg, MB, Canada
6.Univ Manitoba, Dept Biosyst Engn, Winnipeg, MB, Canada
7.Univ Manitoba, Dept Immunol, Winnipeg, MB, Canada
8.Univ Manitoba, Dept Biol Sci, Winnipeg, MB, Canada
Recommended Citation
GB/T 7714
Yang, Ke,Wu, Jiandong,Peretz-Soroka, Hagit,et al. M-kit: A cell migration assay based on microfluidic device and smartphone[J]. BIOSENSORS & BIOELECTRONICS,2018,99(无):259-267.
APA Yang, Ke.,Wu, Jiandong.,Peretz-Soroka, Hagit.,Zhu, Ling.,Li, Zhigang.,...&Lin, Francis.(2018).M-kit: A cell migration assay based on microfluidic device and smartphone.BIOSENSORS & BIOELECTRONICS,99(无),259-267.
MLA Yang, Ke,et al."M-kit: A cell migration assay based on microfluidic device and smartphone".BIOSENSORS & BIOELECTRONICS 99.无(2018):259-267.
Files in This Item: Download All
File Name/Size DocType Version Access License
M-kit_ A cell migrat(1011KB)期刊论文作者接受稿开放获取CC BY-NC-SAView Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Yang, Ke]'s Articles
[Wu, Jiandong]'s Articles
[Peretz-Soroka, Hagit]'s Articles
Baidu academic
Similar articles in Baidu academic
[Yang, Ke]'s Articles
[Wu, Jiandong]'s Articles
[Peretz-Soroka, Hagit]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Yang, Ke]'s Articles
[Wu, Jiandong]'s Articles
[Peretz-Soroka, Hagit]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: M-kit_ A cell migration assay based on microfluidic device and smartphone.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.