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M-kit: A cell migration assay based on microfluidic device and smartphone
Yang, Ke1,2; Wu, Jiandong2; Peretz-Soroka, Hagit2; Zhu, Ling1; Li, Zhigang1; Sang, Yaoshuo1; Hipolito, Jolly2; Zhang, Michael3; Santos, Susy4,5; Hillier, Craig3; de Faria, Ricardo Lobato3; Liu, Yong1; Lin, Francis2,6,7,8
2018-01-15
发表期刊BIOSENSORS & BIOELECTRONICS
卷号99期号:页码:259-267
摘要Mobile sensing based on the integration of microfluidic device and smartphone, so-called MS2 technology, has enabled many applications over recent years, and continues to stimulate growing interest in both research communities and industries. In particular, it has been envisioned that MS2 technology can be developed for various cell functional assays to enable basic research and clinical applications. Toward this direction, in this paper, we describe the development of a MS2-based cell functional assay for testing cell migration (the Midt). The system is constructed as an integrated test kit, which includes microfluidic chips, a smartphone-based imaging platform, the phone apps for image capturing and data analysis, and a set of reagent and accessories for performing the cell migration assay. We demonstrated that the Mkit can effectively measure purified neutrophil and cancer cell chemotaxis. Furthermore, neutrophil chemotaxis can be tested from a drop of whole blood using the Midt with red blood cell (RBC) lysis. The effects of chemoattractant dose and gradient profile on neutrophil chemotaxis were also tested using the Midt. In addition to research applications, we demonstrated the effective use of the Mkit for on-site test at the hospital and for testing clinical samples from chronic obstructive pulmonary disease patient. Thus, this developed Mkit provides an easy and integrated experimental platform for cell migration related research and potential medical diagnostic applications.
文章类型Article
关键词Microfluidic Device Smartphone Cell Functional Assay Cell Migration Chemotaxis
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
资助者Natural Sciences and Engineering Research Council of Canada (NSERC)(RGPIN-2014-04789) ; Canadian Institutes of Health Research (CIHR)(CPG-134755) ; CMC Microsystems(3699) ; Winnipeg Rh Institute Foundation ; University of Manitoba ; Mitacs ; NSERC ; Natural Sciences and Engineering Research Council of Canada (NSERC)(RGPIN-2014-04789) ; Canadian Institutes of Health Research (CIHR)(CPG-134755) ; CMC Microsystems(3699) ; Winnipeg Rh Institute Foundation ; University of Manitoba ; Mitacs ; NSERC
DOI10.1016/j.bios.2017.07.064
关键词[WOS]POINT-OF-CARE ; NEUTROPHIL CHEMOTAXIS ; BIOMEDICAL-RESEARCH ; MINI-MICROSCOPE ; MOBILE PHONE ; BLOOD ; PLATFORM ; SYSTEM ; INFLAMMATION ; TUMOR
收录类别SCI
语种英语
资助者Natural Sciences and Engineering Research Council of Canada (NSERC)(RGPIN-2014-04789) ; Canadian Institutes of Health Research (CIHR)(CPG-134755) ; CMC Microsystems(3699) ; Winnipeg Rh Institute Foundation ; University of Manitoba ; Mitacs ; NSERC ; Natural Sciences and Engineering Research Council of Canada (NSERC)(RGPIN-2014-04789) ; Canadian Institutes of Health Research (CIHR)(CPG-134755) ; CMC Microsystems(3699) ; Winnipeg Rh Institute Foundation ; University of Manitoba ; Mitacs ; NSERC
WOS研究方向Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
WOS类目Biophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology
WOS记录号WOS:000413284200036
引用统计
被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.hfcas.ac.cn:8080/handle/334002/33829
专题应用技术研究所
作者单位1.Chinese Acad Sci, Hefei Inst Phys Sci, Inst Appl Technol, Hefei, Anhui, Peoples R China
2.Univ Manitoba, Dept Phys & Astron, Winnipeg, MB, Canada
3.Seven Oaks Gen Hosp, Winnipeg, MB, Canada
4.Victoria Gen Hosp, Winnipeg, MB, Canada
5.River Hts Ft Garry Community Areas, Winnipeg, MB, Canada
6.Univ Manitoba, Dept Biosyst Engn, Winnipeg, MB, Canada
7.Univ Manitoba, Dept Immunol, Winnipeg, MB, Canada
8.Univ Manitoba, Dept Biol Sci, Winnipeg, MB, Canada
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Yang, Ke,Wu, Jiandong,Peretz-Soroka, Hagit,et al. M-kit: A cell migration assay based on microfluidic device and smartphone[J]. BIOSENSORS & BIOELECTRONICS,2018,99(无):259-267.
APA Yang, Ke.,Wu, Jiandong.,Peretz-Soroka, Hagit.,Zhu, Ling.,Li, Zhigang.,...&Lin, Francis.(2018).M-kit: A cell migration assay based on microfluidic device and smartphone.BIOSENSORS & BIOELECTRONICS,99(无),259-267.
MLA Yang, Ke,et al."M-kit: A cell migration assay based on microfluidic device and smartphone".BIOSENSORS & BIOELECTRONICS 99.无(2018):259-267.
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