Institutional Repository of Chinese Acad Sci, Hefei Inst Phys Sci, Inst Appl Technol,Hefei 230031, Anhui, Peoples R China
M-kit: A cell migration assay based on microfluidic device and smartphone | |
Yang, Ke1,2; Wu, Jiandong2; Peretz-Soroka, Hagit2; Zhu, Ling1; Li, Zhigang1; Sang, Yaoshuo1; Hipolito, Jolly2; Zhang, Michael3; Santos, Susy4,5; Hillier, Craig3; de Faria, Ricardo Lobato3; Liu, Yong1; Lin, Francis2,6,7,8 | |
2018-01-15 | |
发表期刊 | BIOSENSORS & BIOELECTRONICS |
摘要 | Mobile sensing based on the integration of microfluidic device and smartphone, so-called MS2 technology, has enabled many applications over recent years, and continues to stimulate growing interest in both research communities and industries. In particular, it has been envisioned that MS2 technology can be developed for various cell functional assays to enable basic research and clinical applications. Toward this direction, in this paper, we describe the development of a MS2-based cell functional assay for testing cell migration (the Midt). The system is constructed as an integrated test kit, which includes microfluidic chips, a smartphone-based imaging platform, the phone apps for image capturing and data analysis, and a set of reagent and accessories for performing the cell migration assay. We demonstrated that the Mkit can effectively measure purified neutrophil and cancer cell chemotaxis. Furthermore, neutrophil chemotaxis can be tested from a drop of whole blood using the Midt with red blood cell (RBC) lysis. The effects of chemoattractant dose and gradient profile on neutrophil chemotaxis were also tested using the Midt. In addition to research applications, we demonstrated the effective use of the Mkit for on-site test at the hospital and for testing clinical samples from chronic obstructive pulmonary disease patient. Thus, this developed Mkit provides an easy and integrated experimental platform for cell migration related research and potential medical diagnostic applications. |
文章类型 | Article |
关键词 | Microfluidic Device Smartphone Cell Functional Assay Cell Migration Chemotaxis |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
DOI | 10.1016/j.bios.2017.07.064 |
关键词[WOS] | POINT-OF-CARE ; NEUTROPHIL CHEMOTAXIS ; BIOMEDICAL-RESEARCH ; MINI-MICROSCOPE ; MOBILE PHONE ; BLOOD ; PLATFORM ; SYSTEM ; INFLAMMATION ; TUMOR |
收录类别 | SCI |
语种 | 英语 |
项目资助者 | Natural Sciences and Engineering Research Council of Canada (NSERC)(RGPIN-2014-04789) ; Natural Sciences and Engineering Research Council of Canada (NSERC)(RGPIN-2014-04789) ; Natural Sciences and Engineering Research Council of Canada (NSERC)(RGPIN-2014-04789) ; Natural Sciences and Engineering Research Council of Canada (NSERC)(RGPIN-2014-04789) ; Canadian Institutes of Health Research (CIHR)(CPG-134755) ; Canadian Institutes of Health Research (CIHR)(CPG-134755) ; Canadian Institutes of Health Research (CIHR)(CPG-134755) ; Canadian Institutes of Health Research (CIHR)(CPG-134755) ; CMC Microsystems(3699) ; CMC Microsystems(3699) ; CMC Microsystems(3699) ; CMC Microsystems(3699) ; Winnipeg Rh Institute Foundation ; Winnipeg Rh Institute Foundation ; Winnipeg Rh Institute Foundation ; Winnipeg Rh Institute Foundation ; University of Manitoba ; University of Manitoba ; University of Manitoba ; University of Manitoba ; Mitacs ; Mitacs ; Mitacs ; Mitacs ; NSERC ; NSERC ; NSERC ; NSERC |
WOS研究方向 | Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics |
WOS类目 | Biophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology |
WOS记录号 | WOS:000413284200036 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.hfcas.ac.cn:8080/handle/334002/33829 |
专题 | 应用技术研究所 |
作者单位 | 1.Chinese Acad Sci, Hefei Inst Phys Sci, Inst Appl Technol, Hefei, Anhui, Peoples R China 2.Univ Manitoba, Dept Phys & Astron, Winnipeg, MB, Canada 3.Seven Oaks Gen Hosp, Winnipeg, MB, Canada 4.Victoria Gen Hosp, Winnipeg, MB, Canada 5.River Hts Ft Garry Community Areas, Winnipeg, MB, Canada 6.Univ Manitoba, Dept Biosyst Engn, Winnipeg, MB, Canada 7.Univ Manitoba, Dept Immunol, Winnipeg, MB, Canada 8.Univ Manitoba, Dept Biol Sci, Winnipeg, MB, Canada |
推荐引用方式 GB/T 7714 | Yang, Ke,Wu, Jiandong,Peretz-Soroka, Hagit,et al. M-kit: A cell migration assay based on microfluidic device and smartphone[J]. BIOSENSORS & BIOELECTRONICS,2018,99(无):259-267. |
APA | Yang, Ke.,Wu, Jiandong.,Peretz-Soroka, Hagit.,Zhu, Ling.,Li, Zhigang.,...&Lin, Francis.(2018).M-kit: A cell migration assay based on microfluidic device and smartphone.BIOSENSORS & BIOELECTRONICS,99(无),259-267. |
MLA | Yang, Ke,et al."M-kit: A cell migration assay based on microfluidic device and smartphone".BIOSENSORS & BIOELECTRONICS 99.无(2018):259-267. |
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