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Assessment of genotoxicity of four volatile pollutants from cigarette smoke based on the in vitro gamma H2AX assay using high content screening
Zhang, Sen1,2,3; Chen, Huan3; Wang, An1; Liu, Yong1; Hou, Hongwei3; Hu, Qingyuan3
2017-10-01
发表期刊ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
卷号55页码:30-36
摘要To evaluate the genotoxic effects of formaldehyde, acetaldehyde, acrolein and benzene on A549 cells, the in vitro gamma H2AX assay was used in combination with high content screening (HCS) technology. All aldehydes showed a significant genotoxicity in a dose/time-dependent effect on the induction of gamma H2AX. Benzene failed to show a significant genotoxicity based on the gamma H2AX assay. However, hydroquinone (one of metabolites of benzene) showed a significant genotoxicity in vitro. Based on the dose-response of gamma H2AX and Hill model, the ability to induce DNA double-strand break can be evaluated as acrolein > formaldehyde > acetaldehyde > benzene. The slow DNA damage/repair mechanism may be more important than the fast one for aldehydes based on time course of gamma H2AX and two-component model. Overall, all toxicants were genotoxic in a dose- or time-dependent manner based on the in vitro gamma H2AX HCS assay, and acrolein had a strong potential to induce DNA damage followed by formaldehyde, acetaldehyde and benzene in sequence.
文章类型Article
关键词Gamma H2ax High Content Screening Aldehyde Benzene Dose/time-response
WOS标题词Science & Technology ; Life Sciences & Biomedicine
资助者National Natural Science Foundation of China(21347002) ; National Natural Science Foundation of China(21347002) ; National Natural Science Foundation of China(21347002) ; National Natural Science Foundation of China(21347002)
DOI10.1016/j.etap.2017.07.005
关键词[WOS]INDUCED DNA-DAMAGE ; DOSE-RESPONSE
收录类别SCI
语种英语
资助者National Natural Science Foundation of China(21347002) ; National Natural Science Foundation of China(21347002) ; National Natural Science Foundation of China(21347002) ; National Natural Science Foundation of China(21347002)
WOS研究方向Environmental Sciences & Ecology ; Pharmacology & Pharmacy ; Toxicology
WOS类目Environmental Sciences ; Pharmacology & Pharmacy ; Toxicology
WOS记录号WOS:000413383100005
引用统计
文献类型期刊论文
条目标识符http://ir.hfcas.ac.cn:8080/handle/334002/33831
专题应用技术研究所
作者单位1.Chinese Acad Sci, Hefei Inst phys Sci, Inst Appl Technol, Hefei 230088, Anhui, Peoples R China
2.Univ Sci & Technol China, Hefei 230026, Anhui, Peoples R China
3.China Natl Tobacco Qual Supervis & Test Ctr, Zhengzhou 450001, Henan, Peoples R China
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GB/T 7714
Zhang, Sen,Chen, Huan,Wang, An,et al. Assessment of genotoxicity of four volatile pollutants from cigarette smoke based on the in vitro gamma H2AX assay using high content screening[J]. ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY,2017,55:30-36.
APA Zhang, Sen,Chen, Huan,Wang, An,Liu, Yong,Hou, Hongwei,&Hu, Qingyuan.(2017).Assessment of genotoxicity of four volatile pollutants from cigarette smoke based on the in vitro gamma H2AX assay using high content screening.ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY,55,30-36.
MLA Zhang, Sen,et al."Assessment of genotoxicity of four volatile pollutants from cigarette smoke based on the in vitro gamma H2AX assay using high content screening".ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY 55(2017):30-36.
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