Institutional Repository of Chinese Acad Sci, Hefei Inst Phys Sci, Inst Appl Technol，Hefei 230031, Anhui, Peoples R China
| Assessment of genotoxicity of four volatile pollutants from cigarette smoke based on the in vitro gamma H2AX assay using high content screening | |
Zhang, Sen1,2,3; Chen, Huan3; Wang, An1; Liu, Yong1 ; Hou, Hongwei3; Hu, Qingyuan3
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| 2017-10-01 | |
| 发表期刊 | ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
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| 摘要 | To evaluate the genotoxic effects of formaldehyde, acetaldehyde, acrolein and benzene on A549 cells, the in vitro gamma H2AX assay was used in combination with high content screening (HCS) technology. All aldehydes showed a significant genotoxicity in a dose/time-dependent effect on the induction of gamma H2AX. Benzene failed to show a significant genotoxicity based on the gamma H2AX assay. However, hydroquinone (one of metabolites of benzene) showed a significant genotoxicity in vitro. Based on the dose-response of gamma H2AX and Hill model, the ability to induce DNA double-strand break can be evaluated as acrolein > formaldehyde > acetaldehyde > benzene. The slow DNA damage/repair mechanism may be more important than the fast one for aldehydes based on time course of gamma H2AX and two-component model. Overall, all toxicants were genotoxic in a dose- or time-dependent manner based on the in vitro gamma H2AX HCS assay, and acrolein had a strong potential to induce DNA damage followed by formaldehyde, acetaldehyde and benzene in sequence. |
| 文章类型 | Article |
| 关键词 | Gamma H2ax High Content Screening Aldehyde Benzene Dose/time-response |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| DOI | 10.1016/j.etap.2017.07.005 |
| 关键词[WOS] | INDUCED DNA-DAMAGE ; DOSE-RESPONSE |
| 收录类别 | SCI |
| 语种 | 英语 |
| 项目资助者 | National Natural Science Foundation of China(21347002) ; National Natural Science Foundation of China(21347002) ; National Natural Science Foundation of China(21347002) ; National Natural Science Foundation of China(21347002) ; National Natural Science Foundation of China(21347002) ; National Natural Science Foundation of China(21347002) ; National Natural Science Foundation of China(21347002) ; National Natural Science Foundation of China(21347002) |
| WOS研究方向 | Environmental Sciences & Ecology ; Pharmacology & Pharmacy ; Toxicology |
| WOS类目 | Environmental Sciences ; Pharmacology & Pharmacy ; Toxicology |
| WOS记录号 | WOS:000413383100005 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.hfcas.ac.cn:8080/handle/334002/33831 |
| 专题 | 应用技术研究所 |
| 作者单位 | 1.Chinese Acad Sci, Hefei Inst phys Sci, Inst Appl Technol, Hefei 230088, Anhui, Peoples R China 2.Univ Sci & Technol China, Hefei 230026, Anhui, Peoples R China 3.China Natl Tobacco Qual Supervis & Test Ctr, Zhengzhou 450001, Henan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Sen,Chen, Huan,Wang, An,et al. Assessment of genotoxicity of four volatile pollutants from cigarette smoke based on the in vitro gamma H2AX assay using high content screening[J]. ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY,2017,55:30-36. |
| APA | Zhang, Sen,Chen, Huan,Wang, An,Liu, Yong,Hou, Hongwei,&Hu, Qingyuan.(2017).Assessment of genotoxicity of four volatile pollutants from cigarette smoke based on the in vitro gamma H2AX assay using high content screening.ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY,55,30-36. |
| MLA | Zhang, Sen,et al."Assessment of genotoxicity of four volatile pollutants from cigarette smoke based on the in vitro gamma H2AX assay using high content screening".ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY 55(2017):30-36. |
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