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Non-thermal plasma induces mitochondria-mediated apoptotic signaling pathway via ROS generation in HeLa cells
Li, Wei1,8; Yu, K. N.3; Ma, Jie2,4; Shen, Jie5; Cheng, Cheng5; Zhou, Fangjian1; Cai, Zhiming8; Han, Wei2,6,7
2017-11-01
Source PublicationARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
Volume633Issue:Pages:68-77
AbstractNon-thermal plasma (NTP) has been proposed as a novel therapeutic method for anticancer treatment. Although increasing evidence suggests that NTP selectively induces apoptosis in some types of tumor cells, the molecular mechanisms underlying this phenomenon remain unclear. In this study, we further investigated possible molecular mechanisms for NTP-induced apoptosis of HeLa cells. The results showed that NW exposure significantly inhibited the growth and viability of HeLa cells. Morphological observation and flow cytometry analysis demonstrated that NW exposure induced HeLa cell apoptosis. NTP exposure also activated caspase-9 and caspase-3, which subsequently cleaved poly (ADP-ribose) polymerase. Furthermore, NTP exposure suppressed Bcl-2 expression, enhanced Bax expression and trans location to mitochondria, activated mitochondria-mediated apoptotic pathway, followed by the release of cytochrome c. Further studies showed that NTP treatment led to ROS generation, whereas blockade of ROS generation by N-acetyl-L-cysteine (NAC, ROS scavengers) significantly prevented NTP-induced mitochondrial alteration and subsequent apoptosis of HeLa cells via suppressing Bax translocation, cytochrome c and caspase-3 activation. Taken together, our results indicated that NW exposure induced mitochondria-mediated intrinsic apoptosis of HeLa cells was activated by ROS generation. These findings provide insights to the therapeutic potential and clinical research of NW as a novel tool in cervical cancer treatment. (C) 2017 Published by Elsevier Inc.
SubtypeArticle
KeywordNon-thermal Plasma Reactive Oxygen Species Hela Cell Apoptosis Mitochondria
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNatural Science Foundation of China(81227902 ; China Postdoctoral Science Foundation(2016M592584) ; City University of Hong Kong(7004641) ; Priority Academic Program Development of Jiangsu Higher Education Institutions ; Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection ; U1632145) ; Natural Science Foundation of China(81227902 ; China Postdoctoral Science Foundation(2016M592584) ; City University of Hong Kong(7004641) ; Priority Academic Program Development of Jiangsu Higher Education Institutions ; Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection ; U1632145)
DOI10.1016/j.abb.2017.09.005
WOS KeywordCANCER-TREATMENT ; BAX/BCL-2 RATIO ; COLD-PLASMA ; INDUCTION ; MECHANISM ; SUICIDE
Indexed BySCI
Language英语
Funding OrganizationNatural Science Foundation of China(81227902 ; China Postdoctoral Science Foundation(2016M592584) ; City University of Hong Kong(7004641) ; Priority Academic Program Development of Jiangsu Higher Education Institutions ; Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection ; U1632145) ; Natural Science Foundation of China(81227902 ; China Postdoctoral Science Foundation(2016M592584) ; City University of Hong Kong(7004641) ; Priority Academic Program Development of Jiangsu Higher Education Institutions ; Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection ; U1632145)
WOS Research AreaBiochemistry & Molecular Biology ; Biophysics
WOS SubjectBiochemistry & Molecular Biology ; Biophysics
WOS IDWOS:000414381600008
Citation statistics
Document Type期刊论文
Identifierhttp://ir.hfcas.ac.cn:8080/handle/334002/33838
Collection医学物理与技术中心
Affiliation1.Sun Yat Sen Univ, Canc Ctr, Dept Urol, Guangzhou 510060, Guangdong, Peoples R China
2.Chinese Acad Sci, Hefei Inst Phys Sci, Ctr Med Phys & Technol, Hefei, Anhui, Peoples R China
3.City Univ Hong Kong, Dept Phys, Tat Chee Ave, Kowloon Tong, Hong Kong, Peoples R China
4.Univ Sci & Technol China, Hefei, Anhui, Peoples R China
5.Chinese Acad Sci, Inst Plasma Phys, Hefei Inst Phys Sci, Hefei, Anhui, Peoples R China
6.Soochow Univ, Jiangsu Higher Educ Inst, Collaborat Innovat Ctr Radiat Med, Suzhou, Jiangsu, Peoples R China
7.Soochow Univ, Sch Radiol & Interdisciplinary Sci RAD X, Suzhou, Jiangsu, Peoples R China
8.Shenzhen Univ, Affiliated Hosp 1, Shenzhen Peoples Hosp 2, Shenzhen 518036, Peoples R China
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Li, Wei,Yu, K. N.,Ma, Jie,et al. Non-thermal plasma induces mitochondria-mediated apoptotic signaling pathway via ROS generation in HeLa cells[J]. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS,2017,633(无):68-77.
APA Li, Wei.,Yu, K. N..,Ma, Jie.,Shen, Jie.,Cheng, Cheng.,...&Han, Wei.(2017).Non-thermal plasma induces mitochondria-mediated apoptotic signaling pathway via ROS generation in HeLa cells.ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS,633(无),68-77.
MLA Li, Wei,et al."Non-thermal plasma induces mitochondria-mediated apoptotic signaling pathway via ROS generation in HeLa cells".ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS 633.无(2017):68-77.
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