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Conformational change of E-coli sulfurtransferase YgaP upon SCN- in intact native membrane revealed by fluorescence lifetime and anisotropy
Wang, Simeng1; Zhang, Yanan2,3; Zhang, Longhua2,3; Zhang, Min1; Tian, Changlin2,3,4
2018-10-01
Source PublicationCHINESE CHEMICAL LETTERS
ISSN1001-8417
Volume29Issue:10Pages:1513-1516
Corresponding AuthorZhang, Min(zhmin07@ahu.edu.cn) ; Tian, Changlin(cltian@ustc.edu.cn)
AbstractFluorescence lifetime and anisotropy has become a prevalent tool to detect the structure change and motility property of proteins. YgaP is the only membrane-integrated rhodanese in E. coli. The sulfur transfer process has been characterized by various studies. However, the mechanism of the outward transportation of SCN- remains unclear. In this work, we examined the fluorescence lifetime and anisotropy of site-specific incorporated unnatural amino acid 7-HC to study the conformational change of YgaP upon SCN- binding. We also compared the fluorescence changes between detergent-wrapped environment in DPC and intact native membrane environment in SMA. Our results suggested the presence of at least two different conformations in YgaP protein. Both the residues in the middle of TMH2 and the residues near extracellular side play important roles in the binding and/or output of SCN-. SMA is a good material to reflect the in situ conformation changes of protein than micelles. (C) 2018 Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences. Published by Elsevier B.V. All rights reserved.
KeywordRhodanese YgaP Fluorescence lifetime and anisotropy SMA Conformation change
Funding OrganizationNational Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences
DOI10.1016/j.cclet.2018.08.002
WOS KeywordLIGAND-BINDING ; RHODANESE ; PROTEINS ; DYNAMICS ; ACID
Indexed BySCI
Language英语
Funding ProjectNational Key R&D Program of China[2016YFA0400900] ; National Key R&D Program of China[2017YFA0505300] ; Instrument Developing Project of the Chinese Academy of Sciences[YZ201564]
Funding OrganizationNational Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences
WOS Research AreaChemistry
WOS SubjectChemistry, Multidisciplinary
WOS IDWOS:000449448200016
PublisherELSEVIER SCIENCE INC
Citation statistics
Document Type期刊论文
Identifierhttp://ir.hfcas.ac.cn:8080/handle/334002/34155
Collection中科院强磁场科学中心
Corresponding AuthorZhang, Min; Tian, Changlin
Affiliation1.Anhui Univ, Sch Life Sci, Hefei 230039, Anhui, Peoples R China
2.Univ Sci & Technol China, Natl Lab Phys Sci Microscale, Hefei 230027, Anhui, Peoples R China
3.Univ Sci & Technol China, Sch Life Sci, Hefei 230027, Anhui, Peoples R China
4.Chinese Acad Sci, High Field Magnet Lab, Hefei 230031, Anhui, Peoples R China
Recommended Citation
GB/T 7714
Wang, Simeng,Zhang, Yanan,Zhang, Longhua,et al. Conformational change of E-coli sulfurtransferase YgaP upon SCN- in intact native membrane revealed by fluorescence lifetime and anisotropy[J]. CHINESE CHEMICAL LETTERS,2018,29(10):1513-1516.
APA Wang, Simeng,Zhang, Yanan,Zhang, Longhua,Zhang, Min,&Tian, Changlin.(2018).Conformational change of E-coli sulfurtransferase YgaP upon SCN- in intact native membrane revealed by fluorescence lifetime and anisotropy.CHINESE CHEMICAL LETTERS,29(10),1513-1516.
MLA Wang, Simeng,et al."Conformational change of E-coli sulfurtransferase YgaP upon SCN- in intact native membrane revealed by fluorescence lifetime and anisotropy".CHINESE CHEMICAL LETTERS 29.10(2018):1513-1516.
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