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Conformational change of E-coli sulfurtransferase YgaP upon SCN- in intact native membrane revealed by fluorescence lifetime and anisotropy
Wang, Simeng1; Zhang, Yanan2,3; Zhang, Longhua2,3; Zhang, Min1; Tian, Changlin2,3,4
2018-10-01
发表期刊CHINESE CHEMICAL LETTERS
ISSN1001-8417
卷号29期号:10页码:1513-1516
通讯作者Zhang, Min(zhmin07@ahu.edu.cn) ; Tian, Changlin(cltian@ustc.edu.cn)
摘要Fluorescence lifetime and anisotropy has become a prevalent tool to detect the structure change and motility property of proteins. YgaP is the only membrane-integrated rhodanese in E. coli. The sulfur transfer process has been characterized by various studies. However, the mechanism of the outward transportation of SCN- remains unclear. In this work, we examined the fluorescence lifetime and anisotropy of site-specific incorporated unnatural amino acid 7-HC to study the conformational change of YgaP upon SCN- binding. We also compared the fluorescence changes between detergent-wrapped environment in DPC and intact native membrane environment in SMA. Our results suggested the presence of at least two different conformations in YgaP protein. Both the residues in the middle of TMH2 and the residues near extracellular side play important roles in the binding and/or output of SCN-. SMA is a good material to reflect the in situ conformation changes of protein than micelles. (C) 2018 Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences. Published by Elsevier B.V. All rights reserved.
关键词Rhodanese YgaP Fluorescence lifetime and anisotropy SMA Conformation change
资助者National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences
DOI10.1016/j.cclet.2018.08.002
关键词[WOS]LIGAND-BINDING ; RHODANESE ; PROTEINS ; DYNAMICS ; ACID
收录类别SCI
语种英语
资助项目National Key R&D Program of China[2016YFA0400900] ; National Key R&D Program of China[2017YFA0505300] ; Instrument Developing Project of the Chinese Academy of Sciences[YZ201564]
资助者National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences
WOS研究方向Chemistry
WOS类目Chemistry, Multidisciplinary
WOS记录号WOS:000449448200016
出版者ELSEVIER SCIENCE INC
引用统计
文献类型期刊论文
条目标识符http://ir.hfcas.ac.cn:8080/handle/334002/34155
专题中科院强磁场科学中心
通讯作者Zhang, Min; Tian, Changlin
作者单位1.Anhui Univ, Sch Life Sci, Hefei 230039, Anhui, Peoples R China
2.Univ Sci & Technol China, Natl Lab Phys Sci Microscale, Hefei 230027, Anhui, Peoples R China
3.Univ Sci & Technol China, Sch Life Sci, Hefei 230027, Anhui, Peoples R China
4.Chinese Acad Sci, High Field Magnet Lab, Hefei 230031, Anhui, Peoples R China
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GB/T 7714
Wang, Simeng,Zhang, Yanan,Zhang, Longhua,et al. Conformational change of E-coli sulfurtransferase YgaP upon SCN- in intact native membrane revealed by fluorescence lifetime and anisotropy[J]. CHINESE CHEMICAL LETTERS,2018,29(10):1513-1516.
APA Wang, Simeng,Zhang, Yanan,Zhang, Longhua,Zhang, Min,&Tian, Changlin.(2018).Conformational change of E-coli sulfurtransferase YgaP upon SCN- in intact native membrane revealed by fluorescence lifetime and anisotropy.CHINESE CHEMICAL LETTERS,29(10),1513-1516.
MLA Wang, Simeng,et al."Conformational change of E-coli sulfurtransferase YgaP upon SCN- in intact native membrane revealed by fluorescence lifetime and anisotropy".CHINESE CHEMICAL LETTERS 29.10(2018):1513-1516.
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