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In cell measurement of fluorescence lifetime imaging microscopy revealed C-terminal conformation changes of Ferroportin upon addition of Mn2+
Zhang, Mengge1,2; Wen, Ming1,2; Xiong, Ying1,2; Zhang, Longhua1,2; Tian, Changlin1,2,3
2018-10-01
Source PublicationCHINESE CHEMICAL LETTERS
ISSN1001-8417
Volume29Issue:10Pages:1509-1512
Corresponding AuthorZhang, Longhua(zlhustc@ustc.edu.cn) ; Tian, Changlin(cltian@ustc.edu.cn)
AbstractFluorescence microscopy, as a sensitive method to detect microenvironment of molecules, is widely used in protein conformation and dynamic studies in live cells. Fluorescence lifetime imaging microscopy (FLIM), which is independent of fluorophore concentrations, scattering and bleaching, is a suitable tool to analyze membrane proteins in a single cell. Ferroportin (FPN), a multi-ion exporter in vertebrates, was modulated by metal ions with unknown mechanism. Herein, we fused green fluorescence protein on C-terminal of FPN (FPN-eGFP) and applied fluorescence lifetime to monitor conformation changes of FPN in a live cell. The fluorescence lifetime distribution showed a shift to shorter lifetime upon Mn2+ treatment, suggesting a preference conformation of FPN in Mn2+ exposure. It is also observed that the lifetime (rather than intensity) measurement was not strongly influenced by laser power. The observed fluorescence lifetime changes of FPN-eGFP upon Mn2+ treatments indicated that extracellular metal ions can modulate FPN through conformation exchanges between several different states. (C) 2018 Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences. Published by Elsevier B.V. All rights reserved.
KeywordFluorescence lifetime imaging microscopy In cell analysis of conformation change Ferroprotin
Funding OrganizationNational Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences
DOI10.1016/j.cclet.2018.04.026
WOS KeywordIRON EXPORTER ; SPATIAL-RESOLUTION ; TRANSPORTER ; HEPCIDIN ; HOMEOSTASIS ; EXPRESSION ; PROTEINS ; CLONING ; FLIM
Indexed BySCI
Language英语
Funding ProjectNational Key R&D Program of China[2016YFA0400900] ; National Key R&D Program of China[2017YFA0505300] ; Instrument Developing Project of the Chinese Academy of Sciences[YZ201564]
Funding OrganizationNational Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences
WOS Research AreaChemistry
WOS SubjectChemistry, Multidisciplinary
WOS IDWOS:000449448200015
PublisherELSEVIER SCIENCE INC
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.hfcas.ac.cn:8080/handle/334002/34397
Collection中科院强磁场科学中心
Corresponding AuthorZhang, Longhua; Tian, Changlin
Affiliation1.Univ Sci & Technol China, Natl Lab Phys Sci Microscale, Hefei 230027, Anhui, Peoples R China
2.Univ Sci & Technol China, Sch Life Sci, Hefei 230027, Anhui, Peoples R China
3.Chinese Acad Sci, High Field Magnet Lab, Hefei 230031, Anhui, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Mengge,Wen, Ming,Xiong, Ying,et al. In cell measurement of fluorescence lifetime imaging microscopy revealed C-terminal conformation changes of Ferroportin upon addition of Mn2+[J]. CHINESE CHEMICAL LETTERS,2018,29(10):1509-1512.
APA Zhang, Mengge,Wen, Ming,Xiong, Ying,Zhang, Longhua,&Tian, Changlin.(2018).In cell measurement of fluorescence lifetime imaging microscopy revealed C-terminal conformation changes of Ferroportin upon addition of Mn2+.CHINESE CHEMICAL LETTERS,29(10),1509-1512.
MLA Zhang, Mengge,et al."In cell measurement of fluorescence lifetime imaging microscopy revealed C-terminal conformation changes of Ferroportin upon addition of Mn2+".CHINESE CHEMICAL LETTERS 29.10(2018):1509-1512.
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