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A Resource for Inactivation of MicroRNAs Using Short Tandem Target Mimic Technology in Model and Crop Plants
Peng, Ting1,2,3; Qiao, Mengmeng3; Liu, Haiping3; Teotia, Sachin1,3,16; Zhang, Zhanhui1,3; Zhao, Yafan1,2; Wang, Bobo1,2; Zhao, Dongjie3; Shi, Lina1,3; Zhang, Cui4; Le, Brandon4; Rogers, Kestrel4; Gunasekara, Chathura5; Duan, Haitang6; Gu, Yiyou3; Tian, Lei1,3; Nie, Jinfu7,8,9; Qi, Jian7,8; Meng, Fanrong1,3; Huang, Lan10; Chen, Qinghui3,11; Wang, Zhenlin6; Tang, Jinshan12; Tang, Xiaoqing3; Lan, Ting13; Chen, Xuemei4,13; Wei, Hairong5,14; Zhao, Quanzhi1,2; Tang, Guiliang1,3,13,15
2018-11-05
Source PublicationMOLECULAR PLANT
ISSN1674-2052
Volume11Issue:11Pages:1400-1417
Corresponding AuthorChen, Xuemei(xuemei.chen@ucr.edu) ; Wei, Hairong(hairong@mtu.edu) ; Zhao, Quanzhi(qzzhaoh@126.com) ; Tang, Guiliang(gtang1@mtu.edu)
AbstractmicroRNAs (miRNAs) are endogenous small non-coding RNAs that bind to mRNAs and target them for cleavage and/or translational repression, leading to gene silencing. We previously developed short tandem target mimic (STTM) technology to deactivate endogenous miRNAs in Arabidopsis. Here, we created hundreds of STTMs that target both conserved and species-specific miRNAs in Arabidopsis, tomato, rice, and maize, providing a resource for the functional interrogation of miRNAs. We not only revealed the functions of several miRNAs in plant development, but also demonstrated that tissue-specific inactivation of a few miRNAs in rice leads to an increase in grain size without adversely affecting overall plant growth and development. RNA-seq and small RNA-seq analyses of STTM156/157 and STTM165/166 transgenic plants revealed the roles of these miRNAs in plant hormone biosynthesis and activation, secondary metabolism, and ion-channel activity-associated electrophysiology, demonstrating that STTM technology is an effective approach for studying miRNA functions. To facilitate the study and application of STTM transgenic plants and to provide a useful platform for storing and sharing of information about miRNA-regulated gene networks, we have established an online Genome Browser (https://blossom.ffr.mtu.edu/designindex2.php) to display the transcriptomic and miRNAomic changes in STTM-induced miRNA knockdown plants.
Keywordshort tandem target mimic (STTM) miRNA RNA-seq Arabidopsis crop
Funding OrganizationNational Science Foundation, USA ; National Science Foundation, USA ; National Science Foundation, USA ; National Science Foundation, USA ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Major Science and Technology Project of Henan Province ; Major Science and Technology Project of Henan Province ; Major Science and Technology Project of Henan Province ; Major Science and Technology Project of Henan Province ; Support Plan of Science and Technology Innovation Team in Universities of Henan Province ; Support Plan of Science and Technology Innovation Team in Universities of Henan Province ; Support Plan of Science and Technology Innovation Team in Universities of Henan Province ; Support Plan of Science and Technology Innovation Team in Universities of Henan Province ; Key Scientific Research Project in Universities of Henan Province ; Key Scientific Research Project in Universities of Henan Province ; Key Scientific Research Project in Universities of Henan Province ; Key Scientific Research Project in Universities of Henan Province ; Guangdong Innovation Research Team Fund ; Guangdong Innovation Research Team Fund ; Guangdong Innovation Research Team Fund ; Guangdong Innovation Research Team Fund ; 111 Project ; 111 Project ; 111 Project ; 111 Project ; Henan Agricultural University ; Henan Agricultural University ; Henan Agricultural University ; Henan Agricultural University ; China Scholarship Council (CSC) ; China Scholarship Council (CSC) ; China Scholarship Council (CSC) ; China Scholarship Council (CSC) ; National Science Foundation, USA ; National Science Foundation, USA ; National Science Foundation, USA ; National Science Foundation, USA ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Major Science and Technology Project of Henan Province ; Major Science and Technology Project of Henan Province ; Major Science and Technology Project of Henan Province ; Major Science and Technology Project of Henan Province ; Support Plan of Science and Technology Innovation Team in Universities of Henan Province ; Support Plan of Science and Technology Innovation Team in Universities of Henan Province ; Support Plan of Science and Technology Innovation Team in Universities of Henan Province ; Support Plan of Science and Technology Innovation Team in Universities of Henan Province ; Key Scientific Research Project in Universities of Henan Province ; Key Scientific Research Project in Universities of Henan Province ; Key Scientific Research Project in Universities of Henan Province ; Key Scientific Research Project in Universities of Henan Province ; Guangdong Innovation Research Team Fund ; Guangdong Innovation Research Team Fund ; Guangdong Innovation Research Team Fund ; Guangdong Innovation Research Team Fund ; 111 Project ; 111 Project ; 111 Project ; 111 Project ; Henan Agricultural University ; Henan Agricultural University ; Henan Agricultural University ; Henan Agricultural University ; China Scholarship Council (CSC) ; China Scholarship Council (CSC) ; China Scholarship Council (CSC) ; China Scholarship Council (CSC) ; National Science Foundation, USA ; National Science Foundation, USA ; National Science Foundation, USA ; National Science Foundation, USA ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Major Science and Technology Project of Henan Province ; Major Science and Technology Project of Henan Province ; Major Science and Technology Project of Henan Province ; Major Science and Technology Project of Henan Province ; Support Plan of Science and Technology Innovation Team in Universities of Henan Province ; Support Plan of Science and Technology Innovation Team in Universities of Henan Province ; Support Plan of Science and Technology Innovation Team in Universities of Henan Province ; Support Plan of Science and Technology Innovation Team in Universities of Henan Province ; Key Scientific Research Project in Universities of Henan Province ; Key Scientific Research Project in Universities of Henan Province ; Key Scientific Research Project in Universities of Henan Province ; Key Scientific Research Project in Universities of Henan Province ; Guangdong Innovation Research Team Fund ; Guangdong Innovation Research Team Fund ; Guangdong Innovation Research Team Fund ; Guangdong Innovation Research Team Fund ; 111 Project ; 111 Project ; 111 Project ; 111 Project ; Henan Agricultural University ; Henan Agricultural University ; Henan Agricultural University ; Henan Agricultural University ; China Scholarship Council (CSC) ; China Scholarship Council (CSC) ; China Scholarship Council (CSC) ; China Scholarship Council (CSC) ; National Science Foundation, USA ; National Science Foundation, USA ; National Science Foundation, USA ; National Science Foundation, USA ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Major Science and Technology Project of Henan Province ; Major Science and Technology Project of Henan Province ; Major Science and Technology Project of Henan Province ; Major Science and Technology Project of Henan Province ; Support Plan of Science and Technology Innovation Team in Universities of Henan Province ; Support Plan of Science and Technology Innovation Team in Universities of Henan Province ; Support Plan of Science and Technology Innovation Team in Universities of Henan Province ; Support Plan of Science and Technology Innovation Team in Universities of Henan Province ; Key Scientific Research Project in Universities of Henan Province ; Key Scientific Research Project in Universities of Henan Province ; Key Scientific Research Project in Universities of Henan Province ; Key Scientific Research Project in Universities of Henan Province ; Guangdong Innovation Research Team Fund ; Guangdong Innovation Research Team Fund ; Guangdong Innovation Research Team Fund ; Guangdong Innovation Research Team Fund ; 111 Project ; 111 Project ; 111 Project ; 111 Project ; Henan Agricultural University ; Henan Agricultural University ; Henan Agricultural University ; Henan Agricultural University ; China Scholarship Council (CSC) ; China Scholarship Council (CSC) ; China Scholarship Council (CSC) ; China Scholarship Council (CSC)
DOI10.1016/j.molp.2018.09.003
WOS KeywordRICE ORYZA-SATIVA ; GRAIN-SIZE ; ARABIDOPSIS-THALIANA ; SMALL RNAS ; TRANSCRIPTION FACTOR ; PLASMA-MEMBRANE ; GENE-EXPRESSION ; MIRNA ; BIOSYNTHESIS ; EVOLUTION
Indexed BySCI
Language英语
Funding ProjectNational Science Foundation, USA[IOS-1048216] ; National Science Foundation, USA[IOS-1340001] ; National Natural Science Foundation of China[31571679] ; National Natural Science Foundation of China[31501292] ; National Natural Science Foundation of China[31871554] ; Major Science and Technology Project of Henan Province[141100110600] ; Support Plan of Science and Technology Innovation Team in Universities of Henan Province[17IRTSTHN015] ; Key Scientific Research Project in Universities of Henan Province[16A210009] ; Guangdong Innovation Research Team Fund[2014ZT05S078] ; 111 Project[D16014] ; Henan Agricultural University ; China Scholarship Council (CSC)
Funding OrganizationNational Science Foundation, USA ; National Science Foundation, USA ; National Science Foundation, USA ; National Science Foundation, USA ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Major Science and Technology Project of Henan Province ; Major Science and Technology Project of Henan Province ; Major Science and Technology Project of Henan Province ; Major Science and Technology Project of Henan Province ; Support Plan of Science and Technology Innovation Team in Universities of Henan Province ; Support Plan of Science and Technology Innovation Team in Universities of Henan Province ; Support Plan of Science and Technology Innovation Team in Universities of Henan Province ; Support Plan of Science and Technology Innovation Team in Universities of Henan Province ; Key Scientific Research Project in Universities of Henan Province ; Key Scientific Research Project in Universities of Henan Province ; Key Scientific Research Project in Universities of Henan Province ; Key Scientific Research Project in Universities of Henan Province ; Guangdong Innovation Research Team Fund ; Guangdong Innovation Research Team Fund ; Guangdong Innovation Research Team Fund ; Guangdong Innovation Research Team Fund ; 111 Project ; 111 Project ; 111 Project ; 111 Project ; Henan Agricultural University ; Henan Agricultural University ; Henan Agricultural University ; Henan Agricultural University ; China Scholarship Council (CSC) ; China Scholarship Council (CSC) ; China Scholarship Council (CSC) ; China Scholarship Council (CSC) ; National Science Foundation, USA ; National Science Foundation, USA ; National Science Foundation, USA ; National Science Foundation, USA ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Major Science and Technology Project of Henan Province ; Major Science and Technology Project of Henan Province ; Major Science and Technology Project of Henan Province ; Major Science and Technology Project of Henan Province ; Support Plan of Science and Technology Innovation Team in Universities of Henan Province ; Support Plan of Science and Technology Innovation Team in Universities of Henan Province ; Support Plan of Science and Technology Innovation Team in Universities of Henan Province ; Support Plan of Science and Technology Innovation Team in Universities of Henan Province ; Key Scientific Research Project in Universities of Henan Province ; Key Scientific Research Project in Universities of Henan Province ; Key Scientific Research Project in Universities of Henan Province ; Key Scientific Research Project in Universities of Henan Province ; Guangdong Innovation Research Team Fund ; Guangdong Innovation Research Team Fund ; Guangdong Innovation Research Team Fund ; Guangdong Innovation Research Team Fund ; 111 Project ; 111 Project ; 111 Project ; 111 Project ; Henan Agricultural University ; Henan Agricultural University ; Henan Agricultural University ; Henan Agricultural University ; China Scholarship Council (CSC) ; China Scholarship Council (CSC) ; China Scholarship Council (CSC) ; China Scholarship Council (CSC) ; National Science Foundation, USA ; National Science Foundation, USA ; National Science Foundation, USA ; National Science Foundation, USA ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Major Science and Technology Project of Henan Province ; Major Science and Technology Project of Henan Province ; Major Science and Technology Project of Henan Province ; Major Science and Technology Project of Henan Province ; Support Plan of Science and Technology Innovation Team in Universities of Henan Province ; Support Plan of Science and Technology Innovation Team in Universities of Henan Province ; Support Plan of Science and Technology Innovation Team in Universities of Henan Province ; Support Plan of Science and Technology Innovation Team in Universities of Henan Province ; Key Scientific Research Project in Universities of Henan Province ; Key Scientific Research Project in Universities of Henan Province ; Key Scientific Research Project in Universities of Henan Province ; Key Scientific Research Project in Universities of Henan Province ; Guangdong Innovation Research Team Fund ; Guangdong Innovation Research Team Fund ; Guangdong Innovation Research Team Fund ; Guangdong Innovation Research Team Fund ; 111 Project ; 111 Project ; 111 Project ; 111 Project ; Henan Agricultural University ; Henan Agricultural University ; Henan Agricultural University ; Henan Agricultural University ; China Scholarship Council (CSC) ; China Scholarship Council (CSC) ; China Scholarship Council (CSC) ; China Scholarship Council (CSC) ; National Science Foundation, USA ; National Science Foundation, USA ; National Science Foundation, USA ; National Science Foundation, USA ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Major Science and Technology Project of Henan Province ; Major Science and Technology Project of Henan Province ; Major Science and Technology Project of Henan Province ; Major Science and Technology Project of Henan Province ; Support Plan of Science and Technology Innovation Team in Universities of Henan Province ; Support Plan of Science and Technology Innovation Team in Universities of Henan Province ; Support Plan of Science and Technology Innovation Team in Universities of Henan Province ; Support Plan of Science and Technology Innovation Team in Universities of Henan Province ; Key Scientific Research Project in Universities of Henan Province ; Key Scientific Research Project in Universities of Henan Province ; Key Scientific Research Project in Universities of Henan Province ; Key Scientific Research Project in Universities of Henan Province ; Guangdong Innovation Research Team Fund ; Guangdong Innovation Research Team Fund ; Guangdong Innovation Research Team Fund ; Guangdong Innovation Research Team Fund ; 111 Project ; 111 Project ; 111 Project ; 111 Project ; Henan Agricultural University ; Henan Agricultural University ; Henan Agricultural University ; Henan Agricultural University ; China Scholarship Council (CSC) ; China Scholarship Council (CSC) ; China Scholarship Council (CSC) ; China Scholarship Council (CSC)
WOS Research AreaBiochemistry & Molecular Biology ; Plant Sciences
WOS SubjectBiochemistry & Molecular Biology ; Plant Sciences
WOS IDWOS:000449292700007
PublisherCELL PRESS
Citation statistics
Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.hfcas.ac.cn:8080/handle/334002/34409
Collection医学物理与技术中心
Corresponding AuthorChen, Xuemei; Wei, Hairong; Zhao, Quanzhi; Tang, Guiliang
Affiliation1.Henan Agr Univ, Collaborat Innovat Ctr Henan Grain Crops, Zhengzhou 450002, Henan, Peoples R China
2.Henan Agr Univ, Key Lab Rice Biol Henan Prov, Zhengzhou 450002, Henan, Peoples R China
3.Michigan Technol Univ, Life Sci & Technol Inst, Dept Biol Sci, Houghton, MI 49931 USA
4.Univ Calif Riverside, Inst Integrat Genome Biol, Dept Bot & Plant Sci, Riverside, CA 92521 USA
5.Michigan Technol Univ, Sch Forest Resources & Environm Sci, Life Sci & Technol Inst, Houghton, MI 49931 USA
6.Michigan Technol Univ, Dept Comp Sci, Houghton, MI 49931 USA
7.Chinese Acad Sci, Ctr Med Phys & Technol, Hefei Inst Phys Sci, Anhui Prov Key Lab Med Phys & Technol, Hefei, Anhui, Peoples R China
8.Chinese Acad Sci, Hefei Canc Hosp, Hefei, Anhui, Peoples R China
9.Chinese Acad Sci, Guangzhou Inst Biomed & Hlth, Guangzhou, Guangdong, Peoples R China
10.China Agr Univ, Coll Informat & Elect Engn, Beijing 100083, Peoples R China
11.Michigan Technol Univ, Life Sci & Technol Instituted, Dept Kinesiol & Integrat Physiol, Houghton, MI 49931 USA
12.Michigan Technol Univ, Sch Technol, Houghton, MI 49931 USA
13.Shenzhen Univ, Coll Life Sci & Oceanog, Guangdong Prov Key Lab Plant Epigenet, Shenzhen 518060, Peoples R China
14.Beijing Forestry Univ, Beijing Adv Innovat Ctr Tree Breeding Mol Design, Beijing 100083, Peoples R China
15.Henan Univ, Sch Life Sci, Key Lab Plant Stress Biol, State Key Lab Cotton Biol, Kaifeng 475004, Peoples R China
16.Sharda Univ, Dept Biotechnol, Greater Noida 201306, India
Recommended Citation
GB/T 7714
Peng, Ting,Qiao, Mengmeng,Liu, Haiping,et al. A Resource for Inactivation of MicroRNAs Using Short Tandem Target Mimic Technology in Model and Crop Plants[J]. MOLECULAR PLANT,2018,11(11):1400-1417.
APA Peng, Ting.,Qiao, Mengmeng.,Liu, Haiping.,Teotia, Sachin.,Zhang, Zhanhui.,...&Tang, Guiliang.(2018).A Resource for Inactivation of MicroRNAs Using Short Tandem Target Mimic Technology in Model and Crop Plants.MOLECULAR PLANT,11(11),1400-1417.
MLA Peng, Ting,et al."A Resource for Inactivation of MicroRNAs Using Short Tandem Target Mimic Technology in Model and Crop Plants".MOLECULAR PLANT 11.11(2018):1400-1417.
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