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Selenite Reduction and the Biogenesis of Selenium Nanoparticles by Alcaligenes faecalis Se03 Isolated from the Gut of Monochamus alternatus (Coleoptera: Cerambycidae)
Wang, Yuting1,2,3; Shu, Xian1,2; Zhou, Qing1,2; Fan, Tao3; Wang, Taichu3; Chen, Xue1,2; Li, Minghao1,2,4; Ma, Yuhan1,4; Ni, Jun1,4; Hou, Jinyan1,4; Zhao, Weiwei1; Li, Ruixue3; Huang, Shengwei1,4; Wu, Lifang1,3,4
2018-09-01
Source PublicationINTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
ISSN1422-0067
Volume19Issue:9Pages:18
Corresponding AuthorHuang, Shengwei(swhuang@ipp.ac.cn) ; Wu, Lifang(lfwu@ipp.ac.cn)
AbstractIn this study, a bacterial strain exhibiting high selenite (Na2SeO3) tolerance and reduction capacity was isolated from the gut of Monochamus alternatus larvae and identified as Alcaligenes faecalis Se03. The isolate exhibited extreme tolerance to selenite (up to 120 mM) when grown aerobically. In the liquid culture medium, it was capable of reducing nearly 100% of 1.0 and 5.0 mM Na2SeO3 within 24 and 42 h, respectively, leading to the formation of selenium nanoparticles (SeNPs). Electron microscopy and energy dispersive X-ray analysis demonstrated that A. faecalis Se03 produced spherical electron-dense SeNPs with an average hydrodynamic diameter of 273.8 +/- 16.9 nm, localized mainly in the extracellular space. In vitro selenite reduction activity and real-time PCR indicated that proteins such as sulfite reductase and thioredoxin reductase present in the cytoplasm were likely to be involved in selenite reduction and the SeNPs synthesis process in the presence of NADPH or NADH as electron donors. Finally, using Fourier-transform infrared spectrometry, protein and lipid residues were detected on the surface of the biogenic SeNPs. Based on these observations, A. faecalis Se03 has the potential to be an eco-friendly candidate for the bioremediation of selenium-contaminated soil/water and a bacterial catalyst for the biogenesis of SeNPs.
Keywordselenite reduction Alcaligenes faecalis biogenic seleniumnanoparticles electronmicroscopy analysis real-time PCR
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Science and Technology Service Program of the Chinese Academy of Sciences ; Science and Technology Service Program of the Chinese Academy of Sciences ; Science and Technology Service Program of the Chinese Academy of Sciences ; Science and Technology Service Program of the Chinese Academy of Sciences ; key program of 13th five-year plan, CASHIPS ; key program of 13th five-year plan, CASHIPS ; key program of 13th five-year plan, CASHIPS ; key program of 13th five-year plan, CASHIPS ; President Foundation of the Hefei Institutes of Physical Science of the Chinese Academy of Sciences ; President Foundation of the Hefei Institutes of Physical Science of the Chinese Academy of Sciences ; President Foundation of the Hefei Institutes of Physical Science of the Chinese Academy of Sciences ; President Foundation of the Hefei Institutes of Physical Science of the Chinese Academy of Sciences ; major special project of Anhui Province ; major special project of Anhui Province ; major special project of Anhui Province ; major special project of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Scientific and Technological Innovation Team of Anhui Academy of Agricultural Sciences ; Scientific and Technological Innovation Team of Anhui Academy of Agricultural Sciences ; Scientific and Technological Innovation Team of Anhui Academy of Agricultural Sciences ; Scientific and Technological Innovation Team of Anhui Academy of Agricultural Sciences ; Hefei Comprehensive Experimental Station of National Sericultural Industry's Technological System ; Hefei Comprehensive Experimental Station of National Sericultural Industry's Technological System ; Hefei Comprehensive Experimental Station of National Sericultural Industry's Technological System ; Hefei Comprehensive Experimental Station of National Sericultural Industry's Technological System ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Science and Technology Service Program of the Chinese Academy of Sciences ; Science and Technology Service Program of the Chinese Academy of Sciences ; Science and Technology Service Program of the Chinese Academy of Sciences ; Science and Technology Service Program of the Chinese Academy of Sciences ; key program of 13th five-year plan, CASHIPS ; key program of 13th five-year plan, CASHIPS ; key program of 13th five-year plan, CASHIPS ; key program of 13th five-year plan, CASHIPS ; President Foundation of the Hefei Institutes of Physical Science of the Chinese Academy of Sciences ; President Foundation of the Hefei Institutes of Physical Science of the Chinese Academy of Sciences ; President Foundation of the Hefei Institutes of Physical Science of the Chinese Academy of Sciences ; President Foundation of the Hefei Institutes of Physical Science of the Chinese Academy of Sciences ; major special project of Anhui Province ; major special project of Anhui Province ; major special project of Anhui Province ; major special project of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Scientific and Technological Innovation Team of Anhui Academy of Agricultural Sciences ; Scientific and Technological Innovation Team of Anhui Academy of Agricultural Sciences ; Scientific and Technological Innovation Team of Anhui Academy of Agricultural Sciences ; Scientific and Technological Innovation Team of Anhui Academy of Agricultural Sciences ; Hefei Comprehensive Experimental Station of National Sericultural Industry's Technological System ; Hefei Comprehensive Experimental Station of National Sericultural Industry's Technological System ; Hefei Comprehensive Experimental Station of National Sericultural Industry's Technological System ; Hefei Comprehensive Experimental Station of National Sericultural Industry's Technological System ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Science and Technology Service Program of the Chinese Academy of Sciences ; Science and Technology Service Program of the Chinese Academy of Sciences ; Science and Technology Service Program of the Chinese Academy of Sciences ; Science and Technology Service Program of the Chinese Academy of Sciences ; key program of 13th five-year plan, CASHIPS ; key program of 13th five-year plan, CASHIPS ; key program of 13th five-year plan, CASHIPS ; key program of 13th five-year plan, CASHIPS ; President Foundation of the Hefei Institutes of Physical Science of the Chinese Academy of Sciences ; President Foundation of the Hefei Institutes of Physical Science of the Chinese Academy of Sciences ; President Foundation of the Hefei Institutes of Physical Science of the Chinese Academy of Sciences ; President Foundation of the Hefei Institutes of Physical Science of the Chinese Academy of Sciences ; major special project of Anhui Province ; major special project of Anhui Province ; major special project of Anhui Province ; major special project of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Scientific and Technological Innovation Team of Anhui Academy of Agricultural Sciences ; Scientific and Technological Innovation Team of Anhui Academy of Agricultural Sciences ; Scientific and Technological Innovation Team of Anhui Academy of Agricultural Sciences ; Scientific and Technological Innovation Team of Anhui Academy of Agricultural Sciences ; Hefei Comprehensive Experimental Station of National Sericultural Industry's Technological System ; Hefei Comprehensive Experimental Station of National Sericultural Industry's Technological System ; Hefei Comprehensive Experimental Station of National Sericultural Industry's Technological System ; Hefei Comprehensive Experimental Station of National Sericultural Industry's Technological System ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Science and Technology Service Program of the Chinese Academy of Sciences ; Science and Technology Service Program of the Chinese Academy of Sciences ; Science and Technology Service Program of the Chinese Academy of Sciences ; Science and Technology Service Program of the Chinese Academy of Sciences ; key program of 13th five-year plan, CASHIPS ; key program of 13th five-year plan, CASHIPS ; key program of 13th five-year plan, CASHIPS ; key program of 13th five-year plan, CASHIPS ; President Foundation of the Hefei Institutes of Physical Science of the Chinese Academy of Sciences ; President Foundation of the Hefei Institutes of Physical Science of the Chinese Academy of Sciences ; President Foundation of the Hefei Institutes of Physical Science of the Chinese Academy of Sciences ; President Foundation of the Hefei Institutes of Physical Science of the Chinese Academy of Sciences ; major special project of Anhui Province ; major special project of Anhui Province ; major special project of Anhui Province ; major special project of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Scientific and Technological Innovation Team of Anhui Academy of Agricultural Sciences ; Scientific and Technological Innovation Team of Anhui Academy of Agricultural Sciences ; Scientific and Technological Innovation Team of Anhui Academy of Agricultural Sciences ; Scientific and Technological Innovation Team of Anhui Academy of Agricultural Sciences ; Hefei Comprehensive Experimental Station of National Sericultural Industry's Technological System ; Hefei Comprehensive Experimental Station of National Sericultural Industry's Technological System ; Hefei Comprehensive Experimental Station of National Sericultural Industry's Technological System ; Hefei Comprehensive Experimental Station of National Sericultural Industry's Technological System
DOI10.3390/ijms19092799
WOS KeywordELEMENTAL SELENIUM ; AEROBIC CONDITIONS ; YEAST-CELLS ; WASTE-WATER ; PROTEINS ; SELENATE ; BACTERIA ; SOIL ; BIOMINERALIZATION ; BIOSYNTHESIS
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31500531] ; Science and Technology Service Program of the Chinese Academy of Sciences[KFJ-STS-ZDTP-002] ; key program of 13th five-year plan, CASHIPS[kp-2017-21] ; President Foundation of the Hefei Institutes of Physical Science of the Chinese Academy of Sciences[YZJJ201619] ; major special project of Anhui Province[16030701103] ; Natural Science Foundation of Anhui Province[1708085QC70] ; Scientific and Technological Innovation Team of Anhui Academy of Agricultural Sciences[18C0614] ; Hefei Comprehensive Experimental Station of National Sericultural Industry's Technological System[CARS-18-SYZ07]
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Science and Technology Service Program of the Chinese Academy of Sciences ; Science and Technology Service Program of the Chinese Academy of Sciences ; Science and Technology Service Program of the Chinese Academy of Sciences ; Science and Technology Service Program of the Chinese Academy of Sciences ; key program of 13th five-year plan, CASHIPS ; key program of 13th five-year plan, CASHIPS ; key program of 13th five-year plan, CASHIPS ; key program of 13th five-year plan, CASHIPS ; President Foundation of the Hefei Institutes of Physical Science of the Chinese Academy of Sciences ; President Foundation of the Hefei Institutes of Physical Science of the Chinese Academy of Sciences ; President Foundation of the Hefei Institutes of Physical Science of the Chinese Academy of Sciences ; President Foundation of the Hefei Institutes of Physical Science of the Chinese Academy of Sciences ; major special project of Anhui Province ; major special project of Anhui Province ; major special project of Anhui Province ; major special project of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Scientific and Technological Innovation Team of Anhui Academy of Agricultural Sciences ; Scientific and Technological Innovation Team of Anhui Academy of Agricultural Sciences ; Scientific and Technological Innovation Team of Anhui Academy of Agricultural Sciences ; Scientific and Technological Innovation Team of Anhui Academy of Agricultural Sciences ; Hefei Comprehensive Experimental Station of National Sericultural Industry's Technological System ; Hefei Comprehensive Experimental Station of National Sericultural Industry's Technological System ; Hefei Comprehensive Experimental Station of National Sericultural Industry's Technological System ; Hefei Comprehensive Experimental Station of National Sericultural Industry's Technological System ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Science and Technology Service Program of the Chinese Academy of Sciences ; Science and Technology Service Program of the Chinese Academy of Sciences ; Science and Technology Service Program of the Chinese Academy of Sciences ; Science and Technology Service Program of the Chinese Academy of Sciences ; key program of 13th five-year plan, CASHIPS ; key program of 13th five-year plan, CASHIPS ; key program of 13th five-year plan, CASHIPS ; key program of 13th five-year plan, CASHIPS ; President Foundation of the Hefei Institutes of Physical Science of the Chinese Academy of Sciences ; President Foundation of the Hefei Institutes of Physical Science of the Chinese Academy of Sciences ; President Foundation of the Hefei Institutes of Physical Science of the Chinese Academy of Sciences ; President Foundation of the Hefei Institutes of Physical Science of the Chinese Academy of Sciences ; major special project of Anhui Province ; major special project of Anhui Province ; major special project of Anhui Province ; major special project of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Scientific and Technological Innovation Team of Anhui Academy of Agricultural Sciences ; Scientific and Technological Innovation Team of Anhui Academy of Agricultural Sciences ; Scientific and Technological Innovation Team of Anhui Academy of Agricultural Sciences ; Scientific and Technological Innovation Team of Anhui Academy of Agricultural Sciences ; Hefei Comprehensive Experimental Station of National Sericultural Industry's Technological System ; Hefei Comprehensive Experimental Station of National Sericultural Industry's Technological System ; Hefei Comprehensive Experimental Station of National Sericultural Industry's Technological System ; Hefei Comprehensive Experimental Station of National Sericultural Industry's Technological System ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Science and Technology Service Program of the Chinese Academy of Sciences ; Science and Technology Service Program of the Chinese Academy of Sciences ; Science and Technology Service Program of the Chinese Academy of Sciences ; Science and Technology Service Program of the Chinese Academy of Sciences ; key program of 13th five-year plan, CASHIPS ; key program of 13th five-year plan, CASHIPS ; key program of 13th five-year plan, CASHIPS ; key program of 13th five-year plan, CASHIPS ; President Foundation of the Hefei Institutes of Physical Science of the Chinese Academy of Sciences ; President Foundation of the Hefei Institutes of Physical Science of the Chinese Academy of Sciences ; President Foundation of the Hefei Institutes of Physical Science of the Chinese Academy of Sciences ; President Foundation of the Hefei Institutes of Physical Science of the Chinese Academy of Sciences ; major special project of Anhui Province ; major special project of Anhui Province ; major special project of Anhui Province ; major special project of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Scientific and Technological Innovation Team of Anhui Academy of Agricultural Sciences ; Scientific and Technological Innovation Team of Anhui Academy of Agricultural Sciences ; Scientific and Technological Innovation Team of Anhui Academy of Agricultural Sciences ; Scientific and Technological Innovation Team of Anhui Academy of Agricultural Sciences ; Hefei Comprehensive Experimental Station of National Sericultural Industry's Technological System ; Hefei Comprehensive Experimental Station of National Sericultural Industry's Technological System ; Hefei Comprehensive Experimental Station of National Sericultural Industry's Technological System ; Hefei Comprehensive Experimental Station of National Sericultural Industry's Technological System ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Science and Technology Service Program of the Chinese Academy of Sciences ; Science and Technology Service Program of the Chinese Academy of Sciences ; Science and Technology Service Program of the Chinese Academy of Sciences ; Science and Technology Service Program of the Chinese Academy of Sciences ; key program of 13th five-year plan, CASHIPS ; key program of 13th five-year plan, CASHIPS ; key program of 13th five-year plan, CASHIPS ; key program of 13th five-year plan, CASHIPS ; President Foundation of the Hefei Institutes of Physical Science of the Chinese Academy of Sciences ; President Foundation of the Hefei Institutes of Physical Science of the Chinese Academy of Sciences ; President Foundation of the Hefei Institutes of Physical Science of the Chinese Academy of Sciences ; President Foundation of the Hefei Institutes of Physical Science of the Chinese Academy of Sciences ; major special project of Anhui Province ; major special project of Anhui Province ; major special project of Anhui Province ; major special project of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Scientific and Technological Innovation Team of Anhui Academy of Agricultural Sciences ; Scientific and Technological Innovation Team of Anhui Academy of Agricultural Sciences ; Scientific and Technological Innovation Team of Anhui Academy of Agricultural Sciences ; Scientific and Technological Innovation Team of Anhui Academy of Agricultural Sciences ; Hefei Comprehensive Experimental Station of National Sericultural Industry's Technological System ; Hefei Comprehensive Experimental Station of National Sericultural Industry's Technological System ; Hefei Comprehensive Experimental Station of National Sericultural Industry's Technological System ; Hefei Comprehensive Experimental Station of National Sericultural Industry's Technological System
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS IDWOS:000449988100335
PublisherMDPI
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.hfcas.ac.cn:8080/handle/334002/34412
Collection中科院强磁场科学中心
技术生物与农业工程研究所
Corresponding AuthorHuang, Shengwei; Wu, Lifang
Affiliation1.Chinese Acad Sci, Hefei Inst Phys Sci, Key Lab High Magnet Field & Ion Beam Phys Biol, Hefei 230031, Anhui, Peoples R China
2.Univ Sci & Technol China, Sch Life Sci, Hefei 230026, Anhui, Peoples R China
3.Anhui Acad Agr Sci, Sericultural Res Inst, Hefei 230061, Anhui, Peoples R China
4.Chinese Acad Sci, Hefei Inst Phys Sci, Key Lab Environm Toxicol & Pollut Control Technol, Hefei 230031, Anhui, Peoples R China
Recommended Citation
GB/T 7714
Wang, Yuting,Shu, Xian,Zhou, Qing,et al. Selenite Reduction and the Biogenesis of Selenium Nanoparticles by Alcaligenes faecalis Se03 Isolated from the Gut of Monochamus alternatus (Coleoptera: Cerambycidae)[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2018,19(9):18.
APA Wang, Yuting.,Shu, Xian.,Zhou, Qing.,Fan, Tao.,Wang, Taichu.,...&Wu, Lifang.(2018).Selenite Reduction and the Biogenesis of Selenium Nanoparticles by Alcaligenes faecalis Se03 Isolated from the Gut of Monochamus alternatus (Coleoptera: Cerambycidae).INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,19(9),18.
MLA Wang, Yuting,et al."Selenite Reduction and the Biogenesis of Selenium Nanoparticles by Alcaligenes faecalis Se03 Isolated from the Gut of Monochamus alternatus (Coleoptera: Cerambycidae)".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 19.9(2018):18.
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