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Targeting Nrf2-mediated heme oxygenase-1 enhances non-thermal plasma-induced cell death in non-small-cell lung cancer A549 cells
Ma, Jie1,2; Yu, K. N.3; Cheng, Cheng1,4; Ni, Guohua1,4; Shen, Jie1,4; Han, Wei1,5,6
2018-11-15
Source PublicationARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
ISSN0003-9861
Volume658Pages:54-65
Corresponding AuthorHan, Wei(hanw@hfcas.ac.cn)
AbstractNon-thermal plasma (NTP) treatment has been proposed as a potential approach for cancer therapy for killing cancer cells via generation of reactive oxygen species (ROS). As an antioxidant protein, Heme oxygenase-1 (HO 1) has been known to protect cells against oxidative stress. In this paper, we investigated the role of HO-1 activation in NTP-induced apoptosis in A549 cells. Distinctly increased ROS production and apoptosis were observed after NTP exposure. NTP exposure induced HO-1 expression in a dose- and time-dependent manner via activating the translocation of Nuclear factor (erythroid-derived 2)-like 2 (Nrf2) from cytoplasm to nucleus. Furthermore, inhibiting HO-1 activation with its specific inhibitor, ZnPP, increased "killing" effect of NTP. Knocking down HO-1 or Nrf2 with the special siRNA also led to elevated ROS level and enhanced NTP-induced cell death. In addition, the c-JUN N-terminal kinase (JNK) signaling pathway was shown to be involved in NTPinduced HO-1 expression. Interestingly, a higher resistance to NTP exposure of A549 cell compared to H1299 and H322 cells was found to be linked to its higher basal level of HO-1 expression. These findings revealed that HO-1 could be considered as a potential target to improve the effect of NTP in cancer therapy.
KeywordNon-thermal plasma Reactive oxygen species Heme oxygenase-1 Apoptotic death c-JUN N-terminal kinase
Funding OrganizationChinese National Natural Science Foundation ; Chinese National Natural Science Foundation ; Chinese National Natural Science Foundation ; Chinese National Natural Science Foundation ; Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) ; Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) ; Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) ; Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) ; Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection ; Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection ; Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection ; Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection ; City University of Hong Kong ; City University of Hong Kong ; City University of Hong Kong ; City University of Hong Kong ; Chinese National Natural Science Foundation ; Chinese National Natural Science Foundation ; Chinese National Natural Science Foundation ; Chinese National Natural Science Foundation ; Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) ; Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) ; Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) ; Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) ; Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection ; Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection ; Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection ; Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection ; City University of Hong Kong ; City University of Hong Kong ; City University of Hong Kong ; City University of Hong Kong ; Chinese National Natural Science Foundation ; Chinese National Natural Science Foundation ; Chinese National Natural Science Foundation ; Chinese National Natural Science Foundation ; Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) ; Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) ; Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) ; Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) ; Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection ; Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection ; Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection ; Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection ; City University of Hong Kong ; City University of Hong Kong ; City University of Hong Kong ; City University of Hong Kong ; Chinese National Natural Science Foundation ; Chinese National Natural Science Foundation ; Chinese National Natural Science Foundation ; Chinese National Natural Science Foundation ; Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) ; Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) ; Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) ; Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) ; Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection ; Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection ; Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection ; Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection ; City University of Hong Kong ; City University of Hong Kong ; City University of Hong Kong ; City University of Hong Kong
DOI10.1016/j.abb.2018.09.015
WOS KeywordDNA-DAMAGE ; ANTICANCER TREATMENT ; SIGNALING PATHWAY ; HO-1 EXPRESSION ; TUMOR-GROWTH ; HEPG2 CELLS ; ANTIOXIDANT ; INDUCTION ; APOPTOSIS ; NRF2
Indexed BySCI
Language英语
Funding ProjectChinese National Natural Science Foundation[U1632145] ; Chinese National Natural Science Foundation[81227902] ; Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) ; Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection ; City University of Hong Kong[7004641]
Funding OrganizationChinese National Natural Science Foundation ; Chinese National Natural Science Foundation ; Chinese National Natural Science Foundation ; Chinese National Natural Science Foundation ; Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) ; Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) ; Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) ; Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) ; Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection ; Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection ; Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection ; Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection ; City University of Hong Kong ; City University of Hong Kong ; City University of Hong Kong ; City University of Hong Kong ; Chinese National Natural Science Foundation ; Chinese National Natural Science Foundation ; Chinese National Natural Science Foundation ; Chinese National Natural Science Foundation ; Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) ; Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) ; Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) ; Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) ; Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection ; Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection ; Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection ; Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection ; City University of Hong Kong ; City University of Hong Kong ; City University of Hong Kong ; City University of Hong Kong ; Chinese National Natural Science Foundation ; Chinese National Natural Science Foundation ; Chinese National Natural Science Foundation ; Chinese National Natural Science Foundation ; Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) ; Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) ; Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) ; Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) ; Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection ; Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection ; Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection ; Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection ; City University of Hong Kong ; City University of Hong Kong ; City University of Hong Kong ; City University of Hong Kong ; Chinese National Natural Science Foundation ; Chinese National Natural Science Foundation ; Chinese National Natural Science Foundation ; Chinese National Natural Science Foundation ; Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) ; Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) ; Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) ; Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) ; Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection ; Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection ; Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection ; Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection ; City University of Hong Kong ; City University of Hong Kong ; City University of Hong Kong ; City University of Hong Kong
WOS Research AreaBiochemistry & Molecular Biology ; Biophysics
WOS SubjectBiochemistry & Molecular Biology ; Biophysics
WOS IDWOS:000449245400006
PublisherELSEVIER SCIENCE INC
Citation statistics
Document Type期刊论文
Identifierhttp://ir.hfcas.ac.cn:8080/handle/334002/34468
Collection中科院等离子体物理研究所
医学物理与技术中心
Corresponding AuthorHan, Wei
Affiliation1.Chinese Acad Sci, Ctr Med Phys & Technol, Hefei Inst Phys Sci, Anhui Prov Key Lab Med Phys & Technol, Hefei, Anhui, Peoples R China
2.Univ Sci & Technol China, Hefei, Anhui, Peoples R China
3.City Univ Hong Kong, Dept Phys, Tat Chee Ave, Kowloon Tong, Hong Kong, Peoples R China
4.Chinese Acad Sci, Inst Plasma Phys, Hefei Inst Phys Sci, Hefei, Anhui, Peoples R China
5.Soochow Univ, Collaborat Innovat Ctr Radiat Med Jiangsu Higher, Suzhou, Jiangsu, Peoples R China
6.Soochow Univ, Sch Radiol & Interdisciplinary Sci RAD X, Suzhou, Jiangsu, Peoples R China
Recommended Citation
GB/T 7714
Ma, Jie,Yu, K. N.,Cheng, Cheng,et al. Targeting Nrf2-mediated heme oxygenase-1 enhances non-thermal plasma-induced cell death in non-small-cell lung cancer A549 cells[J]. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS,2018,658:54-65.
APA Ma, Jie,Yu, K. N.,Cheng, Cheng,Ni, Guohua,Shen, Jie,&Han, Wei.(2018).Targeting Nrf2-mediated heme oxygenase-1 enhances non-thermal plasma-induced cell death in non-small-cell lung cancer A549 cells.ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS,658,54-65.
MLA Ma, Jie,et al."Targeting Nrf2-mediated heme oxygenase-1 enhances non-thermal plasma-induced cell death in non-small-cell lung cancer A549 cells".ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS 658(2018):54-65.
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