Institutional Repository of Key Laboratory of Ion Beam Bioengineering, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, Anhui 230031, People's Republic of China
Transcriptome analysis of pecan seeds at different developing stages and identification of key genes involved in lipid metabolism | |
Xu, Zheng1; Ni, Jun2; Shah, Faheem Afzal1; Wang, Qiaojian1; Wang, Zhaocheng1; Wu, Lifang2; Fu, Songling1 | |
2018-04-25 | |
发表期刊 | PLOS ONE |
ISSN | 1932-6203 |
摘要 | Pecan is an economically important nut crop tree due to its unique texture and flavor properties. The pecan seed is rich of unsaturated fatty acid and protein. However, little is known about the molecular mechanisms of the biosynthesis of fatty acids in the developing seeds. In this study, transcriptome sequencing of the developing seeds was performed using Illumina sequencing technology. Pecan seed embryos at different developmental stages were collected and sequenced. The transcriptomes of pecan seeds at two key developing stages (PA, the initial stage and PS, the fast oil accumulation stage) were also compared. A total of 82,155 unigenes, with an average length of 1,198 bp from seven independent libraries were generated. After functional annotations, we detected approximately 55,854 CDS, among which, 2,807 were Transcription Factor (TF) coding unigenes. Further, there were 13,325 unigenes that showed a 2-fold or greater expression difference between the two groups of libraries (two developmental stages). After transcriptome analysis, we identified abundant unigenes that could be involved in fatty acid biosynthesis, degradation and some other aspects of seed development in pecan. This study presents a comprehensive dataset of transcriptomic changes during the seed development of pecan. It provides insights in understanding the molecular mechanisms responsible for fatty acid biosynthesis in the seed development. The identification of functional genes will also be useful for the molecular breeding work of pecan. |
DOI | 10.1371/journal.pone.0195913 |
关键词[WOS] | FATTY-ACID-COMPOSITION ; CARYA-ILLINOINENSIS ; RNA-SEQ ; TARGET GENES ; IN-VITRO ; OIL ; PROTEINS ; ARABIDOPSIS ; EXPRESSION ; STABILITY |
收录类别 | SCI |
语种 | 英语 |
资助项目 | Central Finance Forestry Science and Technology Extension Demonstration Fund Project[Anhui [2016] TG02 (1708085QC70)] ; Central Finance Forestry Science and Technology Extension Demonstration Fund Project[Anhui [2016] TG02 (1708085QC70)] |
WOS研究方向 | Science & Technology - Other Topics |
WOS类目 | Multidisciplinary Sciences |
WOS记录号 | WOS:000430802400051 |
出版者 | PUBLIC LIBRARY SCIENCE |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.hfcas.ac.cn:8080/handle/334002/35356 |
专题 | 技术生物与农业工程研究所 |
通讯作者 | Wu, Lifang; Fu, Songling |
作者单位 | 1.Anhui Agr Univ, Coll Forestry & Landscape Architecture, Hefei, Anhui, Peoples R China 2.Chinese Acad Sci, Hefei Inst Phys Sci, Key Lab High Magnet Field & Ion Beam Phys Biol, Hefei, Anhui, Peoples R China |
推荐引用方式 GB/T 7714 | Xu, Zheng,Ni, Jun,Shah, Faheem Afzal,et al. Transcriptome analysis of pecan seeds at different developing stages and identification of key genes involved in lipid metabolism[J]. PLOS ONE,2018,13(4):19. |
APA | Xu, Zheng.,Ni, Jun.,Shah, Faheem Afzal.,Wang, Qiaojian.,Wang, Zhaocheng.,...&Fu, Songling.(2018).Transcriptome analysis of pecan seeds at different developing stages and identification of key genes involved in lipid metabolism.PLOS ONE,13(4),19. |
MLA | Xu, Zheng,et al."Transcriptome analysis of pecan seeds at different developing stages and identification of key genes involved in lipid metabolism".PLOS ONE 13.4(2018):19. |
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