Institutional Repository of Chinese Acad Sci, High Field Magnet Lab,Hefei 230031, Anhui, Peoples R China
Ligand Induced Folding of the First Identified CBM69 Starch Binding Domain AmyP-SBD | |
Zhang, Wei1; Yu, Jigang1; Zhang, Xuecheng1; Peng, Hui1; Li, Xinxin1; Zhang, Jiahai2; Sun, Hongbin3; Tu, Xiaoming2 | |
2018 | |
发表期刊 | PROTEIN AND PEPTIDE LETTERS |
ISSN | 0929-8665 |
通讯作者 | Zhang, Xuecheng(turenzh@ahu.edu.cn) ; Peng, Hui(pph0259@126.com) |
摘要 | AmyP is an alpha-amylase which shows preferential degradation to soluble starch. In this substrate preference its Starch Binding Domain (SBD), which was recently assigned to a new Carbohydrate Binding Module (CBM) family 69, plays an important role. In the present study, the SBD of AmyP (AmyP-SBD) was recombinantly expressed, purified, and structurally characterized. Using Circular Dichroism (CD), intrinsic fluorescence, and nuclear magnetic resonance (NMR) spectroscopy, the structures of AmyP-SBD in the absence and presence of substrate analogue beta-cyclodextrin were measured. The results intriguingly showed that free form AmyP-SBD is partially unfolded, like a compact molten globule, and could be induced by the ligand to fold into a relatively rigid state. Further structure determination for folded AmyP-SBD revealed a topology distinctive from those of SBDs from other CBM families. Our data indicate AmyP-SBD is a structurally novel SBD, and this may be helpful for understanding the properties of AmyP-SBD and CBM69 and elucidation of functioning mechanism of AmyP. |
关键词 | Starch binding domain molten globule ligand induced folding carbohydrate binding module family 69 AmyP nuclear magnetic resonance |
DOI | 10.2174/0929866525666180122145846 |
关键词[WOS] | ASPERGILLUS-NIGER GLUCOAMYLASE ; ALPHA-AMYLASE ENZYMES ; MOLTEN GLOBULE ; NMR ; HYDROLYSIS ; EVOLUTION ; PROTEINS ; GTPASE ; SYSTEM ; FAMILY |
收录类别 | SCI |
语种 | 英语 |
资助项目 | National Natural Science Foundation of China[31470775] ; Key Research Program of the Education Department of Anhui Province[KJ2014ZD01] ; International Science and Technology Cooperation Plan of Anhui Province[1503062010] ; National Natural Science Foundation of China[31470775] ; Key Research Program of the Education Department of Anhui Province[KJ2014ZD01] ; International Science and Technology Cooperation Plan of Anhui Province[1503062010] |
项目资助者 | National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Key Research Program of the Education Department of Anhui Province ; Key Research Program of the Education Department of Anhui Province ; Key Research Program of the Education Department of Anhui Province ; Key Research Program of the Education Department of Anhui Province ; Key Research Program of the Education Department of Anhui Province ; Key Research Program of the Education Department of Anhui Province ; Key Research Program of the Education Department of Anhui Province ; Key Research Program of the Education Department of Anhui Province ; Key Research Program of the Education Department of Anhui Province ; Key Research Program of the Education Department of Anhui Province ; Key Research Program of the Education Department of Anhui Province ; Key Research Program of the Education Department of Anhui Province ; Key Research Program of the Education Department of Anhui Province ; Key Research Program of the Education Department of Anhui Province ; Key Research Program of the Education Department of Anhui Province ; Key Research Program of the Education Department of Anhui Province ; International Science and Technology Cooperation Plan of Anhui Province ; International Science and Technology Cooperation Plan of Anhui Province ; International Science and Technology Cooperation Plan of Anhui Province ; International Science and Technology Cooperation Plan of Anhui Province ; International Science and Technology Cooperation Plan of Anhui Province ; International Science and Technology Cooperation Plan of Anhui Province ; International Science and Technology Cooperation Plan of Anhui Province ; International Science and Technology Cooperation Plan of Anhui Province ; International Science and Technology Cooperation Plan of Anhui Province ; International Science and Technology Cooperation Plan of Anhui Province ; International Science and Technology Cooperation Plan of Anhui Province ; International Science and Technology Cooperation Plan of Anhui Province ; International Science and Technology Cooperation Plan of Anhui Province ; International Science and Technology Cooperation Plan of Anhui Province ; International Science and Technology Cooperation Plan of Anhui Province ; International Science and Technology Cooperation Plan of Anhui Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Key Research Program of the Education Department of Anhui Province ; Key Research Program of the Education Department of Anhui Province ; Key Research Program of the Education Department of Anhui Province ; Key Research Program of the Education Department of Anhui Province ; Key Research Program of the Education Department of Anhui Province ; Key Research Program of the Education Department of Anhui Province ; Key Research Program of the Education Department of Anhui Province ; Key Research Program of the Education Department of Anhui Province ; Key Research Program of the Education Department of Anhui Province ; Key Research Program of the Education Department of Anhui Province ; Key Research Program of the Education Department of Anhui Province ; Key Research Program of the Education Department of Anhui Province ; Key Research Program of the Education Department of Anhui Province ; Key Research Program of the Education Department of Anhui Province ; Key Research Program of the Education Department of Anhui Province ; Key Research Program of the Education Department of Anhui Province ; International Science and Technology Cooperation Plan of Anhui Province ; International Science and Technology Cooperation Plan of Anhui Province ; International Science and Technology Cooperation Plan of Anhui Province ; International Science and Technology Cooperation Plan of Anhui Province ; International Science and Technology Cooperation Plan of Anhui Province ; International Science and Technology Cooperation Plan of Anhui Province ; International Science and Technology Cooperation Plan of Anhui Province ; International Science and Technology Cooperation Plan of Anhui Province ; International Science and Technology Cooperation Plan of Anhui Province ; International Science and Technology Cooperation Plan of Anhui Province ; International Science and Technology Cooperation Plan of Anhui Province ; International Science and Technology Cooperation Plan of Anhui Province ; International Science and Technology Cooperation Plan of Anhui Province ; International Science and Technology Cooperation Plan of Anhui Province ; International Science and Technology Cooperation Plan of Anhui Province ; International Science and Technology Cooperation Plan of Anhui Province |
WOS研究方向 | Biochemistry & Molecular Biology |
WOS类目 | Biochemistry & Molecular Biology |
WOS记录号 | WOS:000435213800006 |
出版者 | BENTHAM SCIENCE PUBL LTD |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.hfcas.ac.cn:8080/handle/334002/38185 |
专题 | 中科院强磁场科学中心 |
通讯作者 | Zhang, Xuecheng; Peng, Hui |
作者单位 | 1.Anhui Univ, Sch Life Sci, 111 Jiulong Rd, Hefei 230601, Anhui, Peoples R China 2.Univ Sci & Technol China, Sch Life Sci, Hefei Natl Lab Phys Sci Microscale, Hefei 230026, Anhui, Peoples R China 3.Chinese Acad Sci, Hefei Inst Phys Sci, High Magnet Field Lab, Hefei 230031, Anhui, Peoples R China |
推荐引用方式 GB/T 7714 | Zhang, Wei,Yu, Jigang,Zhang, Xuecheng,et al. Ligand Induced Folding of the First Identified CBM69 Starch Binding Domain AmyP-SBD[J]. PROTEIN AND PEPTIDE LETTERS,2018,25(4):362-367. |
APA | Zhang, Wei.,Yu, Jigang.,Zhang, Xuecheng.,Peng, Hui.,Li, Xinxin.,...&Tu, Xiaoming.(2018).Ligand Induced Folding of the First Identified CBM69 Starch Binding Domain AmyP-SBD.PROTEIN AND PEPTIDE LETTERS,25(4),362-367. |
MLA | Zhang, Wei,et al."Ligand Induced Folding of the First Identified CBM69 Starch Binding Domain AmyP-SBD".PROTEIN AND PEPTIDE LETTERS 25.4(2018):362-367. |
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