NeuN-Specific Fluorescent Probe Revealing Neuronal Nuclei Protein and Nuclear Acids Association in Living Neurons under STED Nanoscopy

doi:10.1021/acsami.8b11102

	NeuN-Specific Fluorescent Probe Revealing Neuronal Nuclei Protein and Nuclear Acids Association in Living Neurons under STED Nanoscopy
	Tian, Xiaohe 1,2,3; Liu, Tianyan 1; Fang, Bin 3; Wang, Aidong 4; Zhang, Mingzhu 3; Hussain, Sajid 1; Luo, Lei 5; Zhang, Ruilong 3; Zhang, Qiong 3; Wu, Jieying 3; Battaglia, Giuseppe 6; Li, Lin 7,8; Zhang, Zhongping3,9 ; Tian, Yupeng 3
	2018-09-26
发表期刊	ACS APPLIED MATERIALS & INTERFACES
ISSN	1944-8244
通讯作者	Zhang, Zhongping(zpzhang@iim.ac.cn) ; Tian, Yupeng(yptian@ahu.edu.cn)
摘要	Neuronal nuclei (NeuN) protein is a key RNA-associated protein to guide the transcription of RNA and regulate mRNA splicing in neurons. However, the lack of effective labeling and tracking method has hindered the elucidation of the biological mechanism of NeuN operation in living neurons to understand correlated central nervous system disorders. Here, we report a molecular probe that can be inserted into a neighboring hydrophobic-hydrophilic region in NeuN, which upon binding becomes capable of emitting light in red region. The NeuN specificity enables the probe imaging neuronal cells in primary brain regions including hippocampus, cerebellum, midbrain, and cingulate gyrus. The probes' optical properties are such to enable stimulated emission depletion imaging showing for the first time the 3D structure of RNA tangling into NeuN in a living neuron with tens of nanometer resolution.
关键词	fluorescent probe neuronal nuclei protein living neurons STED nanoscopy
DOI	10.1021/acsami.8b11102
关键词[WOS]	CELL ; MICROSCOPY
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[21602003] ; National Natural Science Foundation of China[51432001] ; National Natural Science Foundation of China[51672002] ; National Natural Science Foundation of China[51772002] ; National Natural Science Foundation of China[81503009] ; National Natural Science Foundation of China[21501001] ; Anhui University Doctor Startup Fund[J01001962] ; Anhui Provincial Natural Science Foundation of China[1708085MC68] ; Anhui Provincial Returnees Innovation and Entrepreneurship key support program ; Open fund for Discipline Construction ; Institute of Physical Science and Information Technology, Anhui University ; National Natural Science Foundation of China[21602003] ; National Natural Science Foundation of China[51432001] ; National Natural Science Foundation of China[51672002] ; National Natural Science Foundation of China[51772002] ; National Natural Science Foundation of China[81503009] ; National Natural Science Foundation of China[21501001] ; Anhui University Doctor Startup Fund[J01001962] ; Anhui Provincial Natural Science Foundation of China[1708085MC68] ; Anhui Provincial Returnees Innovation and Entrepreneurship key support program ; Open fund for Discipline Construction ; Institute of Physical Science and Information Technology, Anhui University
项目资助者	National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Anhui University Doctor Startup Fund ; Anhui University Doctor Startup Fund ; Anhui University Doctor Startup Fund ; Anhui University Doctor Startup Fund ; Anhui Provincial Natural Science Foundation of China ; Anhui Provincial Natural Science Foundation of China ; Anhui Provincial Natural Science Foundation of China ; Anhui Provincial Natural Science Foundation of China ; Anhui Provincial Returnees Innovation and Entrepreneurship key support program ; Anhui Provincial Returnees Innovation and Entrepreneurship key support program ; Anhui Provincial Returnees Innovation and Entrepreneurship key support program ; Anhui Provincial Returnees Innovation and Entrepreneurship key support program ; Open fund for Discipline Construction ; Open fund for Discipline Construction ; Open fund for Discipline Construction ; Open fund for Discipline Construction ; Institute of Physical Science and Information Technology, Anhui University ; Institute of Physical Science and Information Technology, Anhui University ; Institute of Physical Science and Information Technology, Anhui University ; Institute of Physical Science and Information Technology, Anhui University ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Anhui University Doctor Startup Fund ; Anhui University Doctor Startup Fund ; Anhui University Doctor Startup Fund ; Anhui University Doctor Startup Fund ; Anhui Provincial Natural Science Foundation of China ; Anhui Provincial Natural Science Foundation of China ; Anhui Provincial Natural Science Foundation of China ; Anhui Provincial Natural Science Foundation of China ; Anhui Provincial Returnees Innovation and Entrepreneurship key support program ; Anhui Provincial Returnees Innovation and Entrepreneurship key support program ; Anhui Provincial Returnees Innovation and Entrepreneurship key support program ; Anhui Provincial Returnees Innovation and Entrepreneurship key support program ; Open fund for Discipline Construction ; Open fund for Discipline Construction ; Open fund for Discipline Construction ; Open fund for Discipline Construction ; Institute of Physical Science and Information Technology, Anhui University ; Institute of Physical Science and Information Technology, Anhui University ; Institute of Physical Science and Information Technology, Anhui University ; Institute of Physical Science and Information Technology, Anhui University
WOS研究方向	Science & Technology - Other Topics ; Materials Science
WOS类目	Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary
WOS记录号	WOS:000446142100015
出版者	AMER CHEMICAL SOC
引用统计	被引频次：17[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.hfcas.ac.cn:8080/handle/334002/39292
专题	中科院合肥智能机械研究所
通讯作者	Zhang, Zhongping; Tian, Yupeng
作者单位	1.Anhui Univ, Sch Life Sci, Hefei 230601, Anhui, Peoples R China 2.Anhui Univ, Inst Phys Sci & Informat Technol, Hefei 230601, Anhui, Peoples R China 3.Anhui Univ, Dept Chem, Anhui Prov Key Lab Chem Inorgan Organ Hybrid Func, Hefei 230601, Anhui, Peoples R China 4.Huangshan Univ, Sch Chem & Chem Engn, Huangshan 245041, Peoples R China 5.Southwest Univ, Coll Pharmaceut Sci, Chongqing 400716, Peoples R China 6.UCL, Dept Chem, London WC1H 0AJ, England 7.Nanjing Tech Univ, Key Lab Flexible Elect, Nanjing 211816, Jiangsu, Peoples R China 8.Nanjing Tech Univ, Inst Adv Mat, Jiangsu Natl Synergist Innovat Ctr Adv Mat SICAM, Nanjing 211816, Jiangsu, Peoples R China 9.Chinese Acad Sci, Inst Intelligent Machines, CAS Ctr Excellence Nanosci, Hefei 230000, Anhui, Peoples R China
推荐引用方式 GB/T 7714	Tian, Xiaohe,Liu, Tianyan,Fang, Bin,et al. NeuN-Specific Fluorescent Probe Revealing Neuronal Nuclei Protein and Nuclear Acids Association in Living Neurons under STED Nanoscopy[J]. ACS APPLIED MATERIALS & INTERFACES,2018,10(38):31959-31964.
APA	Tian, Xiaohe.,Liu, Tianyan.,Fang, Bin.,Wang, Aidong.,Zhang, Mingzhu.,...&Tian, Yupeng.(2018).NeuN-Specific Fluorescent Probe Revealing Neuronal Nuclei Protein and Nuclear Acids Association in Living Neurons under STED Nanoscopy.ACS APPLIED MATERIALS & INTERFACES,10(38),31959-31964.
MLA	Tian, Xiaohe,et al."NeuN-Specific Fluorescent Probe Revealing Neuronal Nuclei Protein and Nuclear Acids Association in Living Neurons under STED Nanoscopy".ACS APPLIED MATERIALS & INTERFACES 10.38(2018):31959-31964.