Institutional Repository of Chinese Acad Sci, High Field Magnet Lab,Hefei 230031, Anhui, Peoples R China
Structural insights into the recognition of phosphorylated Hop1 by Mek1 | |
Xie, Changlin1,2; He, Chao3,4; Jiang, Yiyang2; Yu, Hailong2; Cheng, Lin2; Nshogoza, Gilbert2; Ala, Moududee Sayed2; Tian, Changlin1,2; Wu, Jihui2; Shi, Yunyu1,2; Li, Fudong2 | |
2018-10-01 | |
发表期刊 | ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY |
ISSN | 2059-7983 |
通讯作者 | Shi, Yunyu(yyshi@ustc.edu.cn) ; Li, Fudong(lifudong@ustc.edu.cn) |
摘要 | The FHA domain-containing protein Mek1 is a meiosis-specific kinase that is involved in the regulation of interhomolog recombination in meiosis in Saccharomyces cerevisiae. The recruitment and activation of Mek1 require the phosphorylation of the chromosome axis protein Hop1 at Thr318 (pT318), which is necessary for recognition by the Mek1 FHA domain. Here, crystal structures of the Mek1 FHA domain in the apo state and in complex with the Hop1 pT318 peptide are presented, demonstrating that the hydrophobic residues Phe320 and Val321 at the pT+2 and pT+3 positions in the ligand contribute to the preferential recognition. It was further found that in Schizosaccharomyces pombe Mek1 FHA binds both pT15 in its N-terminal SQ/TQ cluster domain (SCD) and pT270 in the Hop1 SCD. The results revealed the structural basis for the preferential recognition of phosphorylated Hop1 by Mek1 in S. cerevisiae and facilitate the understanding of the interaction between the S. pombe Mek1 FHA domain and its binding targets. |
关键词 | meiotic recombination Mek1 Hop1 FHA domain phosphointeraction |
DOI | 10.1107/S2059798318011993 |
关键词[WOS] | FORKHEAD-ASSOCIATED DOMAIN ; MEIOTIC RECOMBINATION INITIATION ; STRAND BREAK REPAIR ; FHA DOMAIN ; SYNAPTONEMAL COMPLEX ; YEAST MEIOSIS ; SCHIZOSACCHAROMYCES-POMBE ; LINEAR ELEMENTS ; BUDDING YEAST ; INTERHOMOLOG RECOMBINATION |
收录类别 | SCI |
语种 | 英语 |
资助项目 | Chinese National Natural Science Foundation[31330018] ; Chinese National Natural Science Foundation[31500590] ; Chinese National Natural Science Foundation[31400643] ; Ministry of Science and Technology of China[2016YFA0500700] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDB08010101] ; Fundamental Research Funds for the Central Universities[WK2070000095] ; Chinese National Natural Science Foundation[31330018] ; Chinese National Natural Science Foundation[31500590] ; Chinese National Natural Science Foundation[31400643] ; Ministry of Science and Technology of China[2016YFA0500700] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDB08010101] ; Fundamental Research Funds for the Central Universities[WK2070000095] |
项目资助者 | Chinese National Natural Science Foundation ; Chinese National Natural Science Foundation ; Chinese National Natural Science Foundation ; Chinese National Natural Science Foundation ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Chinese National Natural Science Foundation ; Chinese National Natural Science Foundation ; Chinese National Natural Science Foundation ; Chinese National Natural Science Foundation ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities |
WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics ; Crystallography |
WOS类目 | Biochemical Research Methods ; Biochemistry & Molecular Biology ; Biophysics ; Crystallography |
WOS记录号 | WOS:000446420600010 |
出版者 | INT UNION CRYSTALLOGRAPHY |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.hfcas.ac.cn:8080/handle/334002/39513 |
专题 | 中科院强磁场科学中心 |
通讯作者 | Shi, Yunyu; Li, Fudong |
作者单位 | 1.Chinese Acad Sci, High Magnet Field Lab, 50 Shushanhu Rd, Hefei 230031, Anhui, Peoples R China 2.Univ Sci & Technol China, Sch Life Sci, Hefei Natl Lab Phys Sci Microscale, 96 Jinzhai Rd, Hefei 230026, Anhui, Peoples R China 3.Anhui Univ, Anhui Key Lab Modern Biomfg, Hefei 230601, Anhui, Peoples R China 4.Anhui Univ, Sch Life Sci, Hefei 230601, Anhui, Peoples R China |
推荐引用方式 GB/T 7714 | Xie, Changlin,He, Chao,Jiang, Yiyang,et al. Structural insights into the recognition of phosphorylated Hop1 by Mek1[J]. ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY,2018,74:1027-1038. |
APA | Xie, Changlin.,He, Chao.,Jiang, Yiyang.,Yu, Hailong.,Cheng, Lin.,...&Li, Fudong.(2018).Structural insights into the recognition of phosphorylated Hop1 by Mek1.ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY,74,1027-1038. |
MLA | Xie, Changlin,et al."Structural insights into the recognition of phosphorylated Hop1 by Mek1".ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY 74(2018):1027-1038. |
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