Dynamic Nature of CTCF Tandem 11 Zinc Fingers in Multivalent Recognition of DNA As Revealed by NMR Spectroscopy

doi:10.1021/acs.jpclett.8b01440

HFCAS OpenIR > 中科院强磁场科学中心

	Dynamic Nature of CTCF Tandem 11 Zinc Fingers in Multivalent Recognition of DNA As Revealed by NMR Spectroscopy
	Xu, Difei 1; Ma, Rongsheng 1; Zhang, Jiahai 1; Liu, Zhijun 2; Wu, Bo4 ; Peng, Junhui 1; Zhai, Yanan 3; Gong, Qingguo 1; Shi, Yunyu 1; Wu, Jihui 1; Wu, Qiang 3; Zhang, Zhiyong 1; Ruan, Ke 1
	2018-07-19
发表期刊	JOURNAL OF PHYSICAL CHEMISTRY LETTERS
ISSN	1948-7185
通讯作者	Zhang, Zhiyong(zzyzhang@ustc.edu.cn) ; Ruan, Ke(kruan@ustc.edu.cn)
摘要	The 11 zinc fingers (ZFs) of the transcription factor CTCF play a versatile role in the regulation of gene expression. CTCF binds to numerous genomic sites to form chromatin loops and topologically associated domains and thus mediates the 3D architecture of chromatin. Although CTCF inter-ZF plasticity is essential for the recognition of multiple genomic sites, the dynamic nature of its 11 ZFs remains unknown. We assigned the chemical shifts of the CTCF ZFs 1-11 and solved the solution structures of each ZF. NMR backbone dynamics, residual dipolar couplings, and small-angle X-ray scattering experiments suggest a high inter-ZF plasticity of the free-form ZFs 1-11. As exemplified by two different protocadherin DNA sequences, the titration of DNAs to N-15-labeled CTCF ZFs 1-11 enabled systematic mapping of binding of CTCF ZFs to various chromatin sites. Our work paves the way for illustrating the molecular basis of the versatile DNA recognized by CTCF and has interesting implications for its conformational transition during DNA binding.
DOI	10.1021/acs.jpclett.8b01440
关键词[WOS]	RESIDUAL DIPOLAR COUPLINGS ; BIOLOGICAL MACROMOLECULES ; FUNCTIONAL-ORGANIZATION ; CHROMATIN INTERACTIONS ; MAMMALIAN GENOMES ; BINDING ; COHESIN ; GENE ; ALIGNMENT ; PROTEINS
收录类别	SCI
语种	英语
资助项目	Strategic Priority Research Program of the Chinese Academy of Sciences[XDB08030302] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDB08010101] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDPB10] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA12020355] ; Ministry of Science and Technology of China[2016YFA0500700] ; Ministry of Science and Technology of China[2014CB910604] ; National Natural Science Foundation of China[U1632153] ; National Natural Science Foundation of China[21573205] ; National Natural Science Foundation of China[31630039] ; Fundamental Research Funds for the Central Universities[WK2070080002] ; Fundamental Research Funds for the Central Universities[WK2060190086] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDB08030302] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDB08010101] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDPB10] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA12020355] ; Ministry of Science and Technology of China[2016YFA0500700] ; Ministry of Science and Technology of China[2014CB910604] ; National Natural Science Foundation of China[U1632153] ; National Natural Science Foundation of China[21573205] ; National Natural Science Foundation of China[31630039] ; Fundamental Research Funds for the Central Universities[WK2070080002] ; Fundamental Research Funds for the Central Universities[WK2060190086]
项目资助者	Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities
WOS研究方向	Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics
WOS类目	Chemistry, Physical ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Atomic, Molecular & Chemical
WOS记录号	WOS:000448083300033
出版者	AMER CHEMICAL SOC
引用统计	被引频次：14[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.hfcas.ac.cn:8080/handle/334002/39922
专题	中科院强磁场科学中心
通讯作者	Zhang, Zhiyong; Ruan, Ke
作者单位	1.Univ Sci & Technol China, Hefei Natl Lab Phys Sci Microscale, Sch Life Sci, Hefei 230027, Anhui, Peoples R China 2.Chinese Acad Sci, Shanghai Adv Res Inst, ZhangJiang Lab, Natl Facil Prot Sci Shanghai, Shanghai 201210, Peoples R China 3.Shanghai Jiao Tong Univ, Ctr Comparat Biomed,Sch Life Sci & Biotechnol, MOE Key Lab Syst Biomed,SCSB,State Key Lab On Cog, Inst Syst Biomed,Collaborat Innovat Ctr Syst Biom, Shanghai 200240, Peoples R China 4.Chinese Acad Sci, Hefei Inst Phys Sci, High Magnet Field Lab, Hefei 230031, Anhui, Peoples R China
推荐引用方式 GB/T 7714	Xu, Difei,Ma, Rongsheng,Zhang, Jiahai,et al. Dynamic Nature of CTCF Tandem 11 Zinc Fingers in Multivalent Recognition of DNA As Revealed by NMR Spectroscopy[J]. JOURNAL OF PHYSICAL CHEMISTRY LETTERS,2018,9(14):4020-4028.
APA	Xu, Difei.,Ma, Rongsheng.,Zhang, Jiahai.,Liu, Zhijun.,Wu, Bo.,...&Ruan, Ke.(2018).Dynamic Nature of CTCF Tandem 11 Zinc Fingers in Multivalent Recognition of DNA As Revealed by NMR Spectroscopy.JOURNAL OF PHYSICAL CHEMISTRY LETTERS,9(14),4020-4028.
MLA	Xu, Difei,et al."Dynamic Nature of CTCF Tandem 11 Zinc Fingers in Multivalent Recognition of DNA As Revealed by NMR Spectroscopy".JOURNAL OF PHYSICAL CHEMISTRY LETTERS 9.14(2018):4020-4028.