Genotoxicity evaluation of carbon monoxide and 1,3-butadiene using a new joint technology: the in vitro gamma H2AX HCS assay combined with air-liquid interface system

doi:10.1080/15376516.2018.1477897

HFCAS OpenIR > 应用技术研究所

	Genotoxicity evaluation of carbon monoxide and 1,3-butadiene using a new joint technology: the in vitro gamma H2AX HCS assay combined with air-liquid interface system
	Zhang, Sen 1,2,3; Chen, Huan 1; Wang, An 2; Liu, Yong2 ; Hou, Hongwei 1; Hu, Qingyuan 1
	2019-01-02
发表期刊	TOXICOLOGY MECHANISMS AND METHODS
ISSN	1537-6516
通讯作者	Hou, Hongwei(qsfctc@163.com) ; Hu, Qingyuan(1965@163.com)
摘要	To investigate the genotoxicity of gaseous toxicants carbon monoxide (CO) and 1,3-butadiene in vitro, a novel combination technology-the in vitro gamma H2AX high content screening assay combined with air-liquid interface system (ALIS) was established. The results showed that this new technology was available and effective. Based on the joint technology, genotoxicity of CO and 1,3-butadiene was evaluated further in this study. The results showed that treatment concentrations (0, 20%,40%, 80% and 100%, v/v) and exposure time (15, 30, 45, 60 and 90 min) of CO both had no statistically significant effects on the induction of gamma H2AX (p > 0.05). However, 1,3-butadiene can induce significant gamma H2AX (p < 0.01) in A549 cells in a dose/time-dependent manner both in the absence and presence of rat liver S9. When the concentrations of 1,3-butadiene were more than 80%, a higher gamma H2AX level could be induced than the 1.5-fold of vehicle controls after 1 h of treatment. Overall, this new technology can be used as a complementary tool to evaluate the genotoxicity of air-borne toxicants in vitro based on the in vitro gamma H2AX high content screening assay combined with ALIS. Based on the joint technology, CO was not genotoxic in A549 cells, while 1,3-butadiene showed significant genotoxicity in the dose/time-dependency on the induction of gamma H2AX.
关键词	gamma H2AX genotoxicity high content screening carbon monoxide 1,3-butadiene air-liquid interface system
DOI	10.1080/15376516.2018.1477897
关键词[WOS]	CIGARETTE-SMOKE ; EXPOSURE ; MAINSTREAM ; TOXICITY ; IDENTIFICATION ; CYTOTOXICITY ; MUTAGENICITY ; BUTADIENE ; MODEL
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[21347002]
项目资助者	National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China
WOS研究方向	Toxicology
WOS类目	Toxicology
WOS记录号	WOS:000460459500001
出版者	TAYLOR & FRANCIS LTD
引用统计	被引频次：23[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.hfcas.ac.cn:8080/handle/334002/42257
专题	应用技术研究所
通讯作者	Hou, Hongwei; Hu, Qingyuan
作者单位	1.China Natl Tobacco Qual Supervis & Test Ctr, Zhengzhou, Henan, Peoples R China 2.Chinese Acad Sci, Hefei Inst Phys Sci, Inst Appl Technol, Hefei, Anhui, Peoples R China 3.Univ Sci & Technol China, Hefei, Anhui, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Sen,Chen, Huan,Wang, An,et al. Genotoxicity evaluation of carbon monoxide and 1,3-butadiene using a new joint technology: the in vitro gamma H2AX HCS assay combined with air-liquid interface system[J]. TOXICOLOGY MECHANISMS AND METHODS,2019,29(1):1-7.
APA	Zhang, Sen,Chen, Huan,Wang, An,Liu, Yong,Hou, Hongwei,&Hu, Qingyuan.(2019).Genotoxicity evaluation of carbon monoxide and 1,3-butadiene using a new joint technology: the in vitro gamma H2AX HCS assay combined with air-liquid interface system.TOXICOLOGY MECHANISMS AND METHODS,29(1),1-7.
MLA	Zhang, Sen,et al."Genotoxicity evaluation of carbon monoxide and 1,3-butadiene using a new joint technology: the in vitro gamma H2AX HCS assay combined with air-liquid interface system".TOXICOLOGY MECHANISMS AND METHODS 29.1(2019):1-7.