Applications of Locked Nucleic Acid Molecular Beacons in Molecular Recognition and Bioanalysis

HFCAS OpenIR

	Applications of Locked Nucleic Acid Molecular Beacons in Molecular Recognition and Bioanalysis
其他题名	Applications of Locked Nucleic Acid Molecular Beacons in Molecular Recognition and Bioanalysis
	2015
发表期刊	PROGRESS IN CHEMISTRY
ISSN	1005-281X
摘要	Molecular beacons are stem-loop hairpin-structured fluorescence probes with a fluorescent dye at 5' end and a fluorescence quencher at 3' end. When a target complementary sequence is absent, the molecular beacons do not fluoresce, because the formation of the hairpin structure brings the quencher and fluorophore into close proximity, whereby fluorescence is quenched with high efficiency. When a target molecule is present, the hybridization between the target and the loop sequence of the molecular beacon results in the spatial separation of the fluorophore and quencher, which opens the stem-loop structure of molecular beacons to emit fluorescence. Locked nucleic acid is a nucleic acid analogue containing one or more LNA nucleotide monomers with a bicyclic furanose unit locked in an RNA mimicking sugar conformation. It possesses excellent binding affinity to nucleic acid, high biostability and resists to nuclease degradation. Due to the excellent sensitivity and high specificity, the combination of the molecular beacons and locked nucleic acid has aroused wide concern. In this review, we intensively summarize the structure, function, design essentials, current research topics and some important progress. In addition, we also discuss the applications, potential problems and perspective of locked nucleic acid molecular beacons in molecular recognition and bioanalysis.
关键词	SINGLE-NUCLEOTIDE POLYMORPHISMS REAL-TIME PCR MODIFIED ANTISENSE OLIGONUCLEOTIDES IN-VIVO MESSENGER-RNA CHEMICAL-MODIFICATION NUCLEOSIDE ANALOGS NONHUMAN-PRIMATES MAMMALIAN-CELLS LNA locked nucleic acid molecular beacons nucleic acids molecular recognition bioanalysis
收录类别	CSCD
语种	英语
资助项目	[National Natural Science Foundation of China] ; [Special Project in Clinic Medicine of Jiangsu Province] ; [Medicine Leading Talents of Jiangsu Province]
CSCD记录号	CSCD:5590828
引用统计	被引频次：1[CSCD] [CSCD记录]
文献类型	期刊论文
条目标识符	http://ir.hfcas.ac.cn:8080/handle/334002/50554
专题	中国科学院合肥物质科学研究院
推荐引用方式 GB/T 7714	. Applications of Locked Nucleic Acid Molecular Beacons in Molecular Recognition and Bioanalysis[J]. PROGRESS IN CHEMISTRY,2015,27.
APA	(2015).Applications of Locked Nucleic Acid Molecular Beacons in Molecular Recognition and Bioanalysis.PROGRESS IN CHEMISTRY,27.
MLA	"Applications of Locked Nucleic Acid Molecular Beacons in Molecular Recognition and Bioanalysis".PROGRESS IN CHEMISTRY 27(2015).