Mutations in components of the Wnt signaling pathway in gastric cancer

HFCAS OpenIR

	Mutations in components of the Wnt signaling pathway in gastric cancer
其他题名	Mutations in components of the Wnt signaling pathway in gastric cancer
	2008
发表期刊	WORLD JOURNAL OF GASTROENTEROLOGY
ISSN	1007-9327
摘要	AIM: To explore the contribution of AXIN1, AXIN2 and beta-catenin, components of Writ signaling pathway, to the carcinogenesis of gastric cancer (GC), we examined AXIN1, AXIN2 exon7 and CTNNB1 (encoding beta-catenin) exon3 mutations in 70 GCs.
其他摘要	AIM:To explore the contribution of AXIN1,AXIN2 and beta-catenin,components of Wnt signaling pathway,to the carcinogenesis of gastric cancer(GC),we examined AXIN1,AXIN2 exon7 and CTNNB1(encoding betacatenin) exon3 mutations in 70 GCs.METHODS:The presence of mutations was identified by polymerase chain reaction(PCR)-based denaturing high-performance liquid chromatography and direct DNA sequencing.Beta-catenin expression was detected by immunohistochemical analysis.RESULTS:Among the 70 GCs,5(7.1%)had mutations in one or two of these three components.A frameshift mutation(1 bp deletion)in exon7 of AXIN2 was found in one case.Four cases,including the case with a mutation in AXIN2,had frameshift mutations and missense mutations in AXIN1.Five single nucleotide polymorphisms (SNPs),334 C＞T,874 C＞T,1396 G＞A,1690 C＞T and 1942 T＞G,were identified in AXTN1.A frameshift mutation(27 bp deletion) spanning exon3 of CTNNB1 was observed in one case.All four cases with mutations in AXIN1 and AXIN2 showed nuclear betacatenin expression.CONCLUSION:These data indicate that the mutations in AXIN1 and AXIN2 may contribute to gastric carcinogenesis.
关键词	PERFORMANCE LIQUID-CHROMATOGRAPHY BETA-CATENIN MICROSATELLITE INSTABILITY HEPATOCELLULAR CARCINOMAS ADENOMATOUS-POLYPOSIS GERMLINE MUTATIONS COLORECTAL-CANCER AXIN1 GENE APC AXIN1 AXIN2 beta-catenin Wnt signaling pathway gastric cancer
收录类别	CSCD
语种	英语
CSCD记录号	CSCD:3317303
引用统计
文献类型	期刊论文
条目标识符	http://ir.hfcas.ac.cn:8080/handle/334002/53778
专题	中国科学院合肥物质科学研究院
推荐引用方式 GB/T 7714	. Mutations in components of the Wnt signaling pathway in gastric cancer[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2008,14.
APA	(2008).Mutations in components of the Wnt signaling pathway in gastric cancer.WORLD JOURNAL OF GASTROENTEROLOGY,14.
MLA	"Mutations in components of the Wnt signaling pathway in gastric cancer".WORLD JOURNAL OF GASTROENTEROLOGY 14(2008).