Analytical methods for locating modifications in nucleic acids

HFCAS OpenIR

	Analytical methods for locating modifications in nucleic acids
其他题名	Analytical methods for locating modifications in nucleic acids
	2019-01-01
发表期刊	CHINESE CHEMICAL LETTERS
ISSN	1001-8417
摘要	In addition to the canonical nucleobases, a variety of chemical modifications have been identified presence in nucleic acids. These modifications have been demonstrated to involve in regulating the spatiotemporal expression of genes. Up to date, over 150 types of chemical modifications have been found existence in nucleic acids. Understanding the functional roles of modifications relies on deciphering the location information of modifications in nucleic acids. Analytical methods for studying nucleic acid modifications have greatly advanced in the last decade. To locate the modifications in nucleic acids, various mass spectrometry (MS)-based analytical strategies have been established. Recent progress in next-generation sequencing (NGS) in conjugation with immunoprecipitation, chemical reaction, enzyme-mediated mutation, or nanomaterials offer genome-wide or transcriptome-wide mapping of modifications, which greatly revolutionize the field of epigenetic modifications. Herein, we reviewed and summarized the established methods and the breakthrough of the techniques for locating modifications in nucleic acids. In addition, we discussed the principles, applications, advantages and drawbacks of these methods. We believe that with the rapid advancement of techniques and methods, the functions of nucleic acid modifications will be fully understood in the future. (C) 2019 Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences. Published by Elsevier B.V. All rights reserved.
其他摘要	In addition to the canonical nucleobases, a variety of chemical modifications have been identified presence in nucleic acids. These modifications have been demonstrated to involve in regulating the spatiotemporal expression of genes. Up to date, over 150 types of chemical modifications have been found existence in nucleic acids. Understanding the functional roles of modifications relies on deciphering the location information of modifications in nucleic acids. Analytical methods for studying nucleic acid modifications have greatly advanced in the last decade. To locate the modifications in nucleic acids, various mass spectrometry (MS)-based analytical strategies have been established. Recent progress in next-generation sequencing (NGS) in conjugation with immunoprecipitation, chemical reaction, enzyme-mediated mutation, or nanomaterials offer genome-wide or transcriptome-wide mapping of modifications, which greatly revolutionize the field of epigenetic modifications. Herein, we reviewed and summarized the established methods and the breakthrough of the techniques for locating modifications in nucleic acids. In addition, we discussed the principles, applications, advantages and drawbacks of these methods. We believe that with the rapid advancement of techniques and methods, the functions of nucleic acid modifications will be fully understood in the future.
关键词	TANDEM MASS-SPECTROMETRY BASE-RESOLUTION ANALYSIS 23S RIBOSOMAL-RNA LIQUID CHROMATOGRAPHY/MASS SPECTROMETRY SINGLE-NUCLEOTIDE-RESOLUTION ESCHERICHIA-COLI DNA METHYLATION MESSENGER-RNA OXIDATION-PRODUCTS GENOMIC DNA Nucleic acid modification Location Mapping Mass spectrometry Sequencing Nanomaterial
收录类别	CSCD
语种	英语
资助项目	[National Key R&D Program of China] ; [National Natural Science Foundation of China]
CSCD记录号	CSCD:6558242
引用统计	被引频次：10[CSCD] [CSCD记录]
文献类型	期刊论文
条目标识符	http://ir.hfcas.ac.cn:8080/handle/334002/55878
专题	中国科学院合肥物质科学研究院
推荐引用方式 GB/T 7714	. Analytical methods for locating modifications in nucleic acids[J]. CHINESE CHEMICAL LETTERS,2019,30.
APA	(2019).Analytical methods for locating modifications in nucleic acids.CHINESE CHEMICAL LETTERS,30.
MLA	"Analytical methods for locating modifications in nucleic acids".CHINESE CHEMICAL LETTERS 30(2019).