Using intra-operative GeneSearch (TM) Breast Lymph Node Assay to detect breast cancer metastases in sentinel lymph nodes: results from a single institute in China

HFCAS OpenIR

	Using intra-operative GeneSearch (TM) Breast Lymph Node Assay to detect breast cancer metastases in sentinel lymph nodes: results from a single institute in China
其他题名	Using intra-operative GeneSearch~(TM) Breast Lymph Node Assay to detect breast cancer metastases in sentinel lymph nodes: results from a single institute in China
	2011
发表期刊	CHINESE MEDICAL JOURNAL
ISSN	0366-6999
摘要	Background Sentinel lymph node (SLN) biopsy has become a common procedure for early breast cancer patients. The GeneSearch (TM) Breast Lymph Node (BLN) Assay is a real-time RT-PCR assay for the detecting nodal metastases larger than 0.2 mm. China Breast Cancer Clinical Study Group (CBCSG)-001a is a prospective multi-center clinical trial that was conducted to validate the GeneSearch (TM) BLN Assay in China.
其他摘要	Background Sentinel lymph node (SLN) biopsy has become a common procedure for early breast cancer patients. The GeneSearch~(TM) Breast Lymph Node (BLN) Assay is a real-time RT-PCR assay for the detecting nodal metastases larger than 0.2 mm. China Breast Cancer Clinical Study Group (CBCSG)-001a is a prospective multi-center clinical trial that was conducted to validate the GeneSearch~(TM) BLN Assay in China. Methods The SLNs from 90 consecutive patients were identified and dissected, and then sectioned along the short axis into multiple blocks. Intra-operatively, the odd blocks were tested by BLN assay and the even ones were used for frozen section, while all the blocks were evaluated by touch imprint cytology. Post-operatively, the remaining tissues were assessed by histological evaluation. Results A total of 189 SLNs was tested by BLN assay. The sensitivity, specificity, positive predictive value, and negative predictive value were 88.9%, 97.4%, 88.9% and 97.4%, respectively, for BLN assay, 75.0%, 100%, 100% and 94.4%, respectively, for frozen section, and 63.9%, 100%, 100% and 92.2%, respectively, for touch imprint cytology. The sensitivity of BLN assay was higher than that of touch imprint cytology (P=0.01) and frozen section (P=0.13). When assessing the nodes with micro-metastases, BLN assay had a significant higher sensitivity than frozen section (P=0.023) and touch imprint cytology (P=0.005). Conclusion The GeneSearch~(TM) BLN Assay is an accurate and rapid intra-operative assay for breast SLNs and it is suitable for application in general medical practice.
关键词	FROZEN-SECTION MAMMAGLOBIN DISSECTION BIOPSY sentinel lymph node biopsy breast neoplasms intraoperative period polymerase chain reaction histological evaluation
收录类别	CSCD
语种	英语
资助项目	[Johnson & Johnson Medical, China]
CSCD记录号	CSCD:4428495
引用统计
文献类型	期刊论文
条目标识符	http://ir.hfcas.ac.cn:8080/handle/334002/59400
专题	中国科学院合肥物质科学研究院
推荐引用方式 GB/T 7714	. Using intra-operative GeneSearch (TM) Breast Lymph Node Assay to detect breast cancer metastases in sentinel lymph nodes: results from a single institute in China[J]. CHINESE MEDICAL JOURNAL,2011,124.
APA	(2011).Using intra-operative GeneSearch (TM) Breast Lymph Node Assay to detect breast cancer metastases in sentinel lymph nodes: results from a single institute in China.CHINESE MEDICAL JOURNAL,124.
MLA	"Using intra-operative GeneSearch (TM) Breast Lymph Node Assay to detect breast cancer metastases in sentinel lymph nodes: results from a single institute in China".CHINESE MEDICAL JOURNAL 124(2011).