高能中子辐射对线虫的剂量效应研究

HFCAS OpenIR

	高能中子辐射对线虫的剂量效应研究
其他题名	Dose effect of high-energy neutron radiation on Caenorhabditis elegans
	2019-01-01
发表期刊	辐射防护
ISSN	1000-8187
摘要	本文研究了高能中子辐射对秀丽隐杆线虫(Caenorhabditis elegans)剂量效应并讨论了高能中子辐射的相对生物学效应。雌雄同体的线虫随机分为对照组和10个不同剂量梯度的照射组,分别为1 047、476、199、89、18.2、8.4、1.83、0.351、0.171和0.087 Gy。不同剂量梯度的线虫距离中子源的距离不同,但是照射时间相同。线虫经过单次高能中子全身照射后,分别于当天将线虫转入新皿进行产卵率、寿命的后续检测以及24小时后将线虫转入新皿进行生殖细胞凋亡的检测。结果表明,随着剂量的增加,产卵量呈现总体下降的趋势,特别是1.83 Gy对线虫子代数的影响很大,大于89 Gy照射后线虫停止产卵;寿命呈现随辐射剂量上升而下降的趋势,尤其是1.83 Gy对线虫寿命的缩短效应明显;大于8.4 Gy剂量中子照射时,生殖细胞凋亡随剂量的升高而显著上升。以上结果说明,高能中子辐射对线虫具有剂量效应,但是在低剂量辐射时可能有更强的损伤效应,为中子低剂量辐射防护提供了科学依据。本文同时讨论了以中子照射数据与前人γ射线照射实验结果相对比的结果,计算得出HINEG高能中子辐射的相对生物效应是1.25,表明在相近吸收剂量的γ射线与中子照射下两者生物学效应差异,提示了品质因数(Q值)与ICRP出版物的差异以及完善参考动物数据库的必要性。
其他摘要	This paper studies the dose effect of high-energy neutron radiation on Caenorhabditis elegans and discusses the relative biological effects of high-energy neutron radiation.Hermaphrodite C.elegans were randomly divided into control group and 10 irradiation dose groups of 1 047,476,199,89,18.2,8.4,1.83,0.351,0.171 and 0.087 Gy,respectively.C.elegans in each group are placed in different distance from neutron source accordingly with same irradiation time.After a single high-energy neutron whole-body irradiation,part of C.elegans were transferred into the new dish immediately for follow-up detection of spawning rate and lifespan.The other part were transferred 24 hours later for germ cell death detection.The results showed that the spawning rate presented a general decline trend with the increase of dose,especially the damage of 1.83 Gy irradiation on the number of C.elegans progeny.C.elegans with more than 89 Gy radiation stopped spawning.The lifespan of C.elegans showed a trend of decreasing with the increase of radiation dose,particularly the shortening effect of 1.83 Gy was obvious.When irradiated C.elegans with greater than 8.4 Gy,the germ cell death rised significantly with the increase of dose.The above results show that high-energy neutron radiation has a dose effect on C.elegans.There might be a stronger damage effect at low dose,which provides scientific basis for neutron low-dose radiation protection.In this paper,it is also discussed that the relative biological effect of HINEG high-energy neutron radiation is 1.25,which is calculated by comparing the neutron irradiated data with the previousγexperimental results.It showed the biological effect difference betweenγand neutron irradiation.The quality factor(Q)we suggested is different from that of ICRP report.It still needs more effort to improve the reference animal database.
关键词	中子线虫生物学效应低剂量超敏性
收录类别	CSCD
语种	中文
CSCD记录号	CSCD:6474676
引用统计
文献类型	期刊论文
条目标识符	http://ir.hfcas.ac.cn:8080/handle/334002/59883
专题	中国科学院合肥物质科学研究院
推荐引用方式 GB/T 7714	. 高能中子辐射对线虫的剂量效应研究[J]. 辐射防护,2019,039.
APA	(2019).高能中子辐射对线虫的剂量效应研究.辐射防护,039.
MLA	"高能中子辐射对线虫的剂量效应研究".辐射防护 039(2019).