Recombinant adenovirus-p53 (Gendicine) sensitizes a pancreatic carcinoma cell line to radiation

HFCAS OpenIR

	Recombinant adenovirus-p53 (Gendicine) sensitizes a pancreatic carcinoma cell line to radiation
其他题名	Recombinant adenovirus-p53 (Gendicine) sensitizes a pancreatic carcinoma cell line to radiation
	2013
发表期刊	CHINESE JOURNAL OF CANCER RESEARCH
ISSN	1000-9604
摘要	Objective: In this study, we examine the effects of recombinant adenovirus-p53 (rAd-p53) on the pancreatic carcinoma cell line SW1990. Specifically, we determine if expression of rAd-p53 sensitizes these cells to radiation.
其他摘要	Objective In this study, we examine the effects of recombinant adenovirus-p53 (rAd-p53) on the pancreatic carcinoma cell line SW1990. Specifically, we determine if expression of rAd-p53 sensitizes these cells to radiation. Methods Following transfection of SW1990 cells with rAd-p53, we measured expression of P53, P21 and Bax by immunocytochemistry. Both transfected and control cell lines were irradiated with a range of doses, and the survival fractions (SF) were calculated. Dose survival curves were constructed and modeled for comparison. Results Transfection of SW1990 cells with rAd-p53 resulted in increased expression of P53, P21 and Bax in a time-dependent manner. At 96 h after transfection, 89.92% of cells expressed P53, 56.8% expressed P21, and 76.50% expressed Bax. The SF following radiation was lower in the rAd-p53 transfected cells compared to the control cells, suggesting that rAd-p53 sensitizes SW1990 cells to radiation (D 0 for the experimental and control groups was 2.199 and 2.462, respectively). Conclusions Use of the adenoviral vector is an effective means of transfecting SW1990 cells with wild-type P53, and this sensitizes the cell line to irradiation. This work suggests that combining rAd-p53 with radiation therapy in pancreatic cancer may be therapeutically beneficial.
关键词	P53 GENE-TRANSFER ADENOCARCINOMA CELLS TUMOR-REGRESSION CANCER RADIOTHERAPY THERAPY DELIVERY THERAPEUTICS GEMCITABINE COMBINATION Pancreatic carcinoma recombinant adenovirus-p53 (rAd-p53) transfection radiosensitization
收录类别	CSCD
语种	英语
资助项目	[Fujian Province Natural Science Foundation] ; [Beijing Municipal Science & Technology Commission]
CSCD记录号	CSCD:5031826
引用统计
文献类型	期刊论文
条目标识符	http://ir.hfcas.ac.cn:8080/handle/334002/60242
专题	中国科学院合肥物质科学研究院
推荐引用方式 GB/T 7714	. Recombinant adenovirus-p53 (Gendicine) sensitizes a pancreatic carcinoma cell line to radiation[J]. CHINESE JOURNAL OF CANCER RESEARCH,2013,25.
APA	(2013).Recombinant adenovirus-p53 (Gendicine) sensitizes a pancreatic carcinoma cell line to radiation.CHINESE JOURNAL OF CANCER RESEARCH,25.
MLA	"Recombinant adenovirus-p53 (Gendicine) sensitizes a pancreatic carcinoma cell line to radiation".CHINESE JOURNAL OF CANCER RESEARCH 25(2013).