HER2 in gastric cancer: Comparative analysis of three different antibodies using whole-tissue sections and tissue microarrays

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	HER2 in gastric cancer: Comparative analysis of three different antibodies using whole-tissue sections and tissue microarrays
其他题名	HER2 in gastric cancer: Comparative analysis of three different antibodies using whole-tissue sections and tissue microarrays
	2013
发表期刊	WORLD JOURNAL OF GASTROENTEROLOGY
ISSN	1007-9327
摘要	AIM: To compare the performance of three commercially available anti-human epidermalgrowth factor receptor 2 (HER2) antibodies in whole-tissue sections and tissue microarrays (TMAs) of a series of gastric tumors.
其他摘要	AIM:To compare the performance of three commercially available anti-human epidermalgrowth factor receptor 2(HER2)antibodies in whole-tissue sections and tissue microarrays(TMAs)of a series of gastric tumors.METHODS:We present a comparative analysis of three anti-HER2 antibodies(HercepTest,4B5 and SP3)using TMA and whole-tissue sections prepared from the same paraffin blocks of 199 gastric adenocarcinomas operated upon between January 2004 and December2008 at a Brazilian cancer hospital.The data on the patients’age,sex,the anatomical location of the tumor and the Lauren’s histological classification were collected from clinical and pathological records.The immunohistochemical(IHC)results were examined by two pathologists and the cases were classified as positive(3+),equivocal(2+)and negative(0 or 1+),according to the criteria of the IHC scoring system of gastric cancer.TMAs and whole-tissue sections were evaluated separately and independently.All cases yielding discordant IHC results and/or scored as 2+were subjected to dual-color in situ hybridization in order to determine the final HER2 status.Besides determining the sensitivity and predictive value for HER2-positive status,we measured the accuracy of each antibody by calculating the area under the receiver operating characteristic(ROC)curve.The agreement between the results obtained using the TMAs and those obtained using the whole-tissue sections was assessed by means of Kappa coefficient.RESULTS:Intratumoral heterogeneity of HER2 expression was observed with all antibodies.HER2-positive expression(3+)in the whole-tissue sections was observed in 23 cases(11.6%)using the 4B5 antibody,in 18 cases(9.1%)using the SP3 antibody and in 10 cases(5.1%)using the HercepTest antibody.In the TMAs,11 positive cases(5.6%)were identified using SP3 antibody,9(4.6%)using the 4B5 antibody and 6(3%)using the HercepTest antibody.The sensitivity using whole-tissue sections and TMA,respectively,was 95.2%and 42.9%with 4B5,90.5%and 66.7%with SP3 and 47.6%and42.9%with HercepTest.The accuracy,calculated from the area under the ROC curve,using whole-tissue sections and TMA,respectively,was 0.91 and 0.79 by 4B5,0.86 and 0.80 by SP3 and 0.73 and 0.71 by HercepTest.The concordance of the results obtained using wholetissue sections and TMA was 97.4%(Kappa 0.75)using HercepTest,85.6%(Kappa 0.56)using SP3 and 84.1%(Kappa 0.38)using 4B5.CONCLUSION:The use of the 4B5 antibody on wholetissue sections was the most accurate IHC method for evaluating HER2 expression in gastric adenocarcinoma.
关键词	IN-SITU HYBRIDIZATION ROUTINE CLINICAL-PRACTICE GENE AMPLIFICATION BREAST-CANCER EXPRESSION CARCINOMA HER-2/NEU FISH SP3 IMMUNOHISTOCHEMISTRY Gastric cancer Human epidermalgrowth factor receptor 2 Immunohistochemistry Whole-tissue sections Tissue microarray Trastuzumab
收录类别	CSCD
语种	英语
CSCD记录号	CSCD:5009808
引用统计
文献类型	期刊论文
条目标识符	http://ir.hfcas.ac.cn:8080/handle/334002/62740
专题	中国科学院合肥物质科学研究院
推荐引用方式 GB/T 7714	. HER2 in gastric cancer: Comparative analysis of three different antibodies using whole-tissue sections and tissue microarrays[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2013,19.
APA	(2013).HER2 in gastric cancer: Comparative analysis of three different antibodies using whole-tissue sections and tissue microarrays.WORLD JOURNAL OF GASTROENTEROLOGY,19.
MLA	"HER2 in gastric cancer: Comparative analysis of three different antibodies using whole-tissue sections and tissue microarrays".WORLD JOURNAL OF GASTROENTEROLOGY 19(2013).