Autophagy inhibition enhances apigenin-induced apoptosis in human breast cancer cells

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	Autophagy inhibition enhances apigenin-induced apoptosis in human breast cancer cells
其他题名	Autophagy inhibition enhances apigenin-induced apoptosis in human breast cancer cells
	2013
发表期刊	CHINESE JOURNAL OF CANCER RESEARCH
ISSN	1000-9604
摘要	Apigenin (4',5,7-trihydroxyflavone) is a member of the flavone subclass of flavonoids present in fruits and vegetables. The involvement of autophagy in the apigenin-induced apoptotic death of human breast cancer cells was investigated. Cell proliferation and viability were assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and clonogenic assays. Flow cytometry, fluorescent staining and Western blot analysis were employed to detect apoptosis and autophagy, and the role of autophagy was assessed using autophagy inhibitors. Apigenin dose- and time-dependently repressed the proliferation and clonogenic survival of the human breast cancer T47D and MDA-MB-231 cell lines. The death of T47D and MDA-MB-231 cells was due to apoptosis associated with increased levels of Caspase3, PARP cleavage and Bax/Bcl-2 ratios. The results from flow cytometry and fluorescent staining also verified the occurrence of apoptosis. In addition, the apigenin-treated cells exhibited autophagy, as characterized by the appearance of autophagosomes under fluorescence microscopy and the accumulation of acidic vesicular organelles (AVOs) by flow cytometry. Furthermore, the results of the Western blot analysis revealed that the level of LC3-II, the processed form of LC3-I, was increased. Treatment with the autophagy inhibitor, 3-methyladenine (3-MA), significantly enhanced the apoptosis induced by apigenin, which was accompanied by an increase in the level of PARP cleavage. Similar results were also confirmed by flow cytometry and fluorescence microscopy. These results indicate that apigenin has apoptosis- and autophagy-inducing effects in breast cancer cells. Autophagy plays a cyto-protective role in apigenin-induced apoptosis, and the combination of apigenin and an autophagy inhibitor may be a promising strategy for breast cancer control.
其他摘要	Apigenin (4',5,7-trihydroxyflavone) is a member of the flavone subclass of flavonoids present in fruits and vegetables. The involvement of autophagy in the apigenin-induced apoptotic death of human breast cancer cells was investigated. Cell proliferation and viability were assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and clonogenic assays. Flow cytometry, fluorescent staining and Western blot analysis were employed to detect apoptosis and autophagy, and the role of autophagy was assessed using autophagy inhibitors. Apigenin dose- and time-dependently repressed the proliferation and clonogenic survival of the human breast cancer T47D and MDA-MB-231 cell lines. The death of T47D and MDA-MB-231 cells was due to apoptosis associated with increased levels of Caspase3, PARP cleavage and Bax
关键词	CYCLE ARREST MOLECULAR-MECHANISMS ARSENIC TRIOXIDE SELF-DIGESTION NUDE MICE EXPRESSION INDUCTION DEATH CHEMOPREVENTION CHEMOTHERAPY Apoptosis autophagy apigenin breast cancer 3-methyladenine
收录类别	CSCD
语种	英语
资助项目	[National Natural Science Foundation of China] ; [Tianjin Municipal Natural Science Foundation]
CSCD记录号	CSCD:4829480
引用统计
文献类型	期刊论文
条目标识符	http://ir.hfcas.ac.cn:8080/handle/334002/65129
专题	中国科学院合肥物质科学研究院
推荐引用方式 GB/T 7714	. Autophagy inhibition enhances apigenin-induced apoptosis in human breast cancer cells[J]. CHINESE JOURNAL OF CANCER RESEARCH,2013,25.
APA	(2013).Autophagy inhibition enhances apigenin-induced apoptosis in human breast cancer cells.CHINESE JOURNAL OF CANCER RESEARCH,25.
MLA	"Autophagy inhibition enhances apigenin-induced apoptosis in human breast cancer cells".CHINESE JOURNAL OF CANCER RESEARCH 25(2013).