Assessment of the Escherichia coli Tat protein translocation system with fluorescent proteins

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	Assessment of the Escherichia coli Tat protein translocation system with fluorescent proteins
其他题名	Assessment of the Escherichia coli Tat Protein Translocation System with Fluorescent Proteins
	2003
发表期刊	ACTA BIOCHIMICA ET BIOPHYSICA SINICA
ISSN	0582-9879
摘要	The possibility of using fluorescent proteins as probes to study the twin-arginine translocation (Tat) system was assessed in Escherichia coli. When fused to the twin-arginine signal peptide of trimethylamine N-oxide reductase, the DsRed2 red fluorescent protein from the Discosoma sp. was successfully synthesized and folded in E. coli cells. However, RR-DsRed2 aggregated inside the cells. Therefore, although DsRed2 has been engineered from DsRed for faster maturation and lower non-specific aggregation, it is still not compatible with Tat-dependent translocation. In contrast, the jellyfish green fluorescent protein (GFP) was efficiently exported into periplasm even when the RR motif was changed to KR or RK These results show that GFP can be used as an efficient reporter protein to study Tat system, but DsRed2 is not suitable for such purpose because of its aggregation property. In addition, when the protein concentration was similar, the fluorescence intensity of KR-GFP and RK-GFP decreased compared with RR-GFP, which would suggest that the twin-arginine signal peptide is not only essential for mediating protein translocation, but also important for the folding of down-stream protein.
关键词	TWIN-ARGININE TRANSLOCASE SITE-DIRECTED MUTAGENESIS EXPORT PATHWAY SIGNAL PEPTIDE LOCALIZATION COMPONENTS BINDING COMPLEX CORAL FORM fluorescent protein report protein Tat protein translocation signal peptide protein folding
收录类别	CSCD
语种	英语
CSCD记录号	CSCD:1247664
引用统计
文献类型	期刊论文
条目标识符	http://ir.hfcas.ac.cn:8080/handle/334002/69095
专题	中国科学院合肥物质科学研究院
推荐引用方式 GB/T 7714	. Assessment of the Escherichia coli Tat protein translocation system with fluorescent proteins[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2003,35.
APA	(2003).Assessment of the Escherichia coli Tat protein translocation system with fluorescent proteins.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,35.
MLA	"Assessment of the Escherichia coli Tat protein translocation system with fluorescent proteins".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 35(2003).