Circular Bispecific Aptamer-Mediated Artificial Intercellular Recognition for Targeted T Cell Immunotherapy

doi:10.1021/acsnano.9b09884

HFCAS OpenIR

	Circular Bispecific Aptamer-Mediated Artificial Intercellular Recognition for Targeted T Cell Immunotherapy
	Yang, Yu1,2,3,4 ; Sun, Xiaoqi 5; Xu, Jun 6; Cui, Cheng 3,4; Yazd, Hoda Safari 3; Pan, Xiaoshu 3; Zhu, Yujie 6; Chen, Xigao 3,4; Li, Xiaowei 3,4; Li, Jin 7,8,9; Tan, Weihong 1,2,7
	2020-08-25
发表期刊	ACS NANO
ISSN	1936-0851
通讯作者	Cui, Cheng(cuichengcuicheng@gmail.com) ; Tan, Weihong(tan@hnu.edu.cn)
摘要	Adoptive T cell immunotherapy, such as chimeric antigen receptor (CAR) T cell therapy, has proven to be highly efficient in the treatment of hematologic malignancies. However, it is challenged by complicated ex vivo engineering, systemic side effects, and low expression of tumor-specific antigen, especially in solid tumors. In this paper, we present a "recognition-then-activation" strategy, which first assists naive T cells to recognize and adhere to cancer cells and then activates the accumulated T cell in situ to specifically kill cancer cells. In this way, we could unleash the antitumor power of the T cell without complicated and time-consuming cell engineering. To this end, circular bispecific aptamers (cb-aptamers), a class of chemically cyclized aptamers with improved stability and molecular recognition ability which can simultaneously bind to two different types of cells, were first constructed to form artificial intercellular recognition between naive T cells and tumor cells. After T cell accumulation in the tumor mediated by cb-aptamers, T cells in the tumor site were subsequently activated in situ via commercial CD3/CD28 T cell activator beads to induce tumor-specific killing. Furthermore, by simply choosing different anticancer aptamers, the application of this "recognition-then-activation" strategy can be expanded for targeted treatment of various types of cancer. This may represent a simple T cell immunotherapy that is useful for the treatment of multiple cancers.
关键词	recognition-then-activation circular bispecific aptamer cb-aptamer adoptive T cell immunotherapy T cell immunotherapy on shelf
DOI	10.1021/acsnano.9b09884
关键词[WOS]	CANCER-IMMUNOTHERAPY ; ADOPTIVE IMMUNOTHERAPY ; ANTIBODIES ; RECEPTOR ; THERAPY ; SELECTION ; MELANOMA ; ANTIGENS ; B7-H1
收录类别	SCI
语种	英语
资助项目	National Institutes of Health[GM079359] ; National Institutes of Health[P0077690] ; National Institutes of Health[CA133086] ; NSFC[21505032] ; NSFC[21325520] ; NSFC[1327009]
项目资助者	National Institutes of Health ; NSFC
WOS研究方向	Chemistry ; Science & Technology - Other Topics ; Materials Science
WOS类目	Chemistry, Multidisciplinary ; Chemistry, Physical ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary
WOS记录号	WOS:000566341000025
出版者	AMER CHEMICAL SOC
引用统计	被引频次：66[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.hfcas.ac.cn:8080/handle/334002/70454
专题	中国科学院合肥物质科学研究院
通讯作者	Cui, Cheng; Tan, Weihong
作者单位	1.Shanghai Jiao Tong Univ, Renji Hosp, Inst Mol Med IMM,Sch Med, State Key Lab Oncogenes & Related Genes, Shanghai 200240, Peoples R China 2.Shanghai Jiao Tong Univ, Coll Chem & Chem Engn, Shanghai 200240, Peoples R China 3.Univ Florida, Dept Chem, Ctr Res Bio Nano Interface, Hlth Canc Ctr,UF Genet Inst,Dept Physiol & Funct, Gainesville, FL 32611 USA 4.Univ Florida, McKnight Brain Inst, Gainesville, FL 32611 USA 5.Univ Michigan, Dept Pharmaceut Sci, Ann Arbor, MI 48109 USA 6.Soochow Univ, Inst Funct Nano & Soft Mat Lab FUNSOM, Suzhou 215123, Peoples R China 7.Hunan Univ, Coll Chem & Chem Engn,Coll Biol, Aptamer Engn Ctr Hunan Prov, Mol Sci & Biomed Lab MBL,State Key Lab Chemo Bios, Changsha 410082, Peoples R China 8.Univ Chinese Acad Sci, Zhejiang Canc Hosp, Canc Hosp, Hangzhou 310022, Zhejiang, Peoples R China 9.Chinese Acad Sci, Inst Canc & Basic Med IBMC, Hangzhou 310022, Zhejiang, Peoples R China
推荐引用方式 GB/T 7714	Yang, Yu,Sun, Xiaoqi,Xu, Jun,et al. Circular Bispecific Aptamer-Mediated Artificial Intercellular Recognition for Targeted T Cell Immunotherapy[J]. ACS NANO,2020,14.
APA	Yang, Yu.,Sun, Xiaoqi.,Xu, Jun.,Cui, Cheng.,Yazd, Hoda Safari.,...&Tan, Weihong.(2020).Circular Bispecific Aptamer-Mediated Artificial Intercellular Recognition for Targeted T Cell Immunotherapy.ACS NANO,14.
MLA	Yang, Yu,et al."Circular Bispecific Aptamer-Mediated Artificial Intercellular Recognition for Targeted T Cell Immunotherapy".ACS NANO 14(2020).