Aptamer-based optical manipulation of protein subcellular localization in cells

doi:10.1038/s41467-020-15113-2

HFCAS OpenIR

	Aptamer-based optical manipulation of protein subcellular localization in cells
	Xie, Sitao 1; Du, Yulin 1; Zhang, Yu 1; Wang, Zhimin 1; Zhang, Dailiang 1; He, Lei 1; Qiu, Liping 1; Jiang, Jianhui 1; Tan, Weihong 1,2,3,4
	2020-03-12
发表期刊	NATURE COMMUNICATIONS
ISSN	2041-1723
通讯作者	Qiu, Liping(qiuliping@hnu.edu.cn) ; Jiang, Jianhui(jianhuijiang@hnu.edu.cn) ; Tan, Weihong(tan@hnu.edu.cn)
摘要	Protein-dominant cellular processes cannot be fully decoded without precise manipulation of their activity and localization in living cells. Advances in optogenetics have allowed spatio-temporal control over cellular proteins with molecular specificity; however, these methods require recombinant expression of fusion proteins, possibly leading to conflicting results. Instead of modifying proteins of interest, in this work, we focus on design of a tunable recognition unit and develop an aptamer-based near-infrared (NIR) light-responsive nano-platform for manipulating the subcellular localization of specific proteins in their native states. Our results demonstrate that this nanoplatform allows photocontrol over the cytoplasmicnuclear shuttling behavior of the target RelA protein (a member of the NF-kappa beta family), enabling regulation of RelA-related signaling pathways. With a modular design, this aptamer-based nanoplatform can be readily extended for the manipulation of different proteins (e.g., lyso-zyme and p53), holding great potential to develop a variety of label-free protein photoregulation strategies for studying complex biological events.
DOI	10.1038/s41467-020-15113-2
关键词[WOS]	OPTOGENETIC CONTROL ; UP-CONVERSION ; DNA ; SELECTION ; ALPHA ; SPACE
收录类别	SCI
语种	英语
资助项目	NSFC[NSFC 21827811] ; NSFC[21527810] ; NSFC[21922404] ; National Key Research Program[2019YFA0905800] ; Science and Technology Project of Hunan Province[2017XK2103] ; Hunan Provincial Innovation Foundation For Postgraduate
项目资助者	NSFC ; National Key Research Program ; Science and Technology Project of Hunan Province ; Hunan Provincial Innovation Foundation For Postgraduate
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000563527600008
出版者	NATURE PUBLISHING GROUP
引用统计	被引频次：40[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.hfcas.ac.cn:8080/handle/334002/70498
专题	中国科学院合肥物质科学研究院
通讯作者	Qiu, Liping; Jiang, Jianhui; Tan, Weihong
作者单位	1.Hunan Univ, Collaborat Innovat Ctr Chem & Mol Med, Coll Chem & Chem Engn,Mol Sci & Biomed Lab MBL, Coll Biol,State Key Lab Chemo Biosensing & Chemom, Changsha 410082, Peoples R China 2.Chinese Acad Sci, Canc Hosp, Univ Chinese Acad Sci, Inst Canc & Basic Med IBMC, Hangzhou 310022, Zhejiang, Peoples R China 3.Shanghai Jiao Tong Univ, Renji Hosp, State Key Lab Oncogenes & Related Genes, Sch Med,Inst Mol Med IMM, Shanghai 200240, Peoples R China 4.Shanghai Jiao Tong Univ, Coll Chem & Chem Engn, Shanghai 200240, Peoples R China
推荐引用方式 GB/T 7714	Xie, Sitao,Du, Yulin,Zhang, Yu,et al. Aptamer-based optical manipulation of protein subcellular localization in cells[J]. NATURE COMMUNICATIONS,2020,11.
APA	Xie, Sitao.,Du, Yulin.,Zhang, Yu.,Wang, Zhimin.,Zhang, Dailiang.,...&Tan, Weihong.(2020).Aptamer-based optical manipulation of protein subcellular localization in cells.NATURE COMMUNICATIONS,11.
MLA	Xie, Sitao,et al."Aptamer-based optical manipulation of protein subcellular localization in cells".NATURE COMMUNICATIONS 11(2020).