HFCAS OpenIR  > 中科院安徽光学精密机械研究所
A Novel Microfluidic Device Integrated with Chitosan-Modified Capillaries for Rapid ZIKV Detection
朱灵( zhu ling)
2020-02-11
Source PublicationMicromachines
Corresponding AuthorYang, Ke(keyang@rntek.cas.cn) ; Zhu, Ling(zhul@aiofm.ac.cn)
AbstractThe outbreak of Zika virus (ZIKV) has posed a great challenge to public health in recent years. To address the urgent need of ZIKV RNA assays, we integrate the microfluidic chip embedded with chitosan-modified silicon dioxide capillaries, smartphone-based detection unit to be a C-3-system for the rapid extraction and detection of ZIKV RNA. The C-3-system is characterized by: (1) four chitosan-modified silicon dioxide capillaries integrated in the microfluidic chip for target ZIKV RNA enrichment and "in situ PCR" (polymerase chain reaction) amplification; (2) smartphone-based point of care (POC) device consisting of a pneumatic subsystem for controlling the nucleic acid extraction processes in the microfluidic chip, a heating subsystem for sample lysis and PCR amplification, and an optical subsystem for signal acquisition. The entire detection processes including sample lysis, ZIKV RNA enrichment, and reverse-transcription polymerase chain reaction (RT-PCR) is achieved in the microfluidic chip. Moreover, PCR buffers can be directly loaded into the chitosan-modified silicon dioxide capillaries for "in situ PCR", in which the captured ZIKV RNA is directly used for downstream PCR without any loss. ZIKV RNA extracted by the C-3-system can be successfully recovered at very low concentrations of 50 transducing units (TU)/mL from crude human saliva. This means that our method of detecting viremia in patients infected with ZIKV is reliable.
Keywordin situ PCR molecular diagnosis microfluidic chip POC device ZIKV
DOI10.3390/mi11020186
WOS KeywordNUCLEIC-ACID EXTRACTION ; WHOLE-BLOOD ; AMPLIFICATION ; DNA ; CAPTURE ; IDENTIFICATION ; ADSORPTION ; EFFICIENT ; VIRUS
Indexed BySCI
Language英语
Funding ProjectClass C talent fund project of Chinese Academy of Sciences[CXJJ-19-C03] ; Dean's Fund of Hefei Institute of Physical Science, China[YZJJ2018QN22] ; Open Subject of Key Laboratory of Optoelectronic Materials Science and Technology of Anhui[OMST201701] ; Key Research and Development Program of Anhui[1804a09020051] ; Key Research and Development Program of Anhui[1804c04020335] ; Key Research and Development Program of Anhui[201904c03020001] ; Youth Innovation Promotion Association of the Chinese Academy of Sciences[2018489]
Funding OrganizationClass C talent fund project of Chinese Academy of Sciences ; Dean's Fund of Hefei Institute of Physical Science, China ; Open Subject of Key Laboratory of Optoelectronic Materials Science and Technology of Anhui ; Key Research and Development Program of Anhui ; Youth Innovation Promotion Association of the Chinese Academy of Sciences
WOS Research AreaScience & Technology - Other Topics ; Instruments & Instrumentation
WOS SubjectNanoscience & Nanotechnology ; Instruments & Instrumentation
WOS IDWOS:000520181500075
PublisherMDPI
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.hfcas.ac.cn:8080/handle/334002/92924
Collection中科院安徽光学精密机械研究所
Corresponding Author朱灵( zhu ling)
Recommended Citation
GB/T 7714
Zhu L. A Novel Microfluidic Device Integrated with Chitosan-Modified Capillaries for Rapid ZIKV Detection[J]. Micromachines,2020,11.
APA 朱灵.(2020).A Novel Microfluidic Device Integrated with Chitosan-Modified Capillaries for Rapid ZIKV Detection.Micromachines,11.
MLA 朱灵."A Novel Microfluidic Device Integrated with Chitosan-Modified Capillaries for Rapid ZIKV Detection".Micromachines 11(2020).
Files in This Item: Download All
File Name/Size DocType Version Access License
4 A_Novel_Microfluid(3906KB)期刊论文作者接受稿开放获取CC BY-NC-SAView Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[朱灵( zhu ling)]'s Articles
Baidu academic
Similar articles in Baidu academic
[朱灵( zhu ling)]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[朱灵( zhu ling)]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: 4 A_Novel_Microfluidic_Device_Integrated_with_Chitos.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.